Antibody domain exchange is an immunological solution to carbohydrate cluster recognition

Science

Volumn 300, Issue 5628, 2003, Pages 2065-2071

  Calarese, Daniel A ^a   Scanlan, Christopher N ^a,b   Zwick, Michael B ^a   Deechongkit, Songpon ^a   Mimura, Yusuke ^b   Kunert, Renate ^c   Zhu, Ping ^d   Wormald, Mark R ^b   Stanfield, Robyn L ^a   Roux, Kenneth H ^d   Kelly, Jeffery W ^a   Rudd, Pauline M ^b   Dwek, Raymond A ^b   Katinger, Hermann ^c   Burton, Dennis R ^a   Wilson, Ian A ^a  

^a SCRIPPS RESEARCH INSTITUTE   (United States)

^b UNIVERSITY OF OXFORD   (United Kingdom)

^c UNIVERSITY OF AGRICULTURAL SCIENCES   (Austria)

^d FLORIDA STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMISTRY; CARBOHYDRATES; CRYSTAL STRUCTURE; DIMERS; IMMUNOLOGY; MUTAGENESIS; VIRUSES;

IMMUNOLOGICAL SOLUTION;

ANTIBODIES;

ANTIBODY 2G12; CARBOHYDRATE; DIMER; DISACCHARIDE; EPITOPE; GLYCOPROTEIN GP 120; IMMUNOGLOBULIN F(AB) FRAGMENT; MANNOSE; NEUTRALIZING ANTIBODY; OLIGOSACCHARIDE; UNCLASSIFIED DRUG; VIRUS ENVELOPE PROTEIN;

ANTIBODY; HUMAN IMMUNODEFICIENCY VIRUS;

ANTIBODY COMBINING SITE; ANTIGEN BINDING; ARTICLE; BIOCHEMISTRY; BIOPHYSICS; CELL SURFACE; CRYSTAL STRUCTURE; HUMAN; HUMAN IMMUNODEFICIENCY VIRUS 1; IMMUNITY; MUTAGENESIS; NONHUMAN; PRIORITY JOURNAL; PROTEIN DOMAIN;

AMINO ACID SEQUENCE; ANTIBODIES, MONOCLONAL; ANTIBODY AFFINITY; ANTIBODY SPECIFICITY; BINDING SITES, ANTIBODY; CELL ADHESION MOLECULES; CENTRIFUGATION, DENSITY GRADIENT; CRYSTALLIZATION; CRYSTALLOGRAPHY, X-RAY; DIMERIZATION; DISACCHARIDES; EPITOPES; HIV ANTIBODIES; HIV ENVELOPE PROTEIN GP120; HIV-1; HUMANS; HYDROGEN BONDING; IMMUNOGLOBULIN FAB FRAGMENTS; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN LIGHT CHAINS; IMMUNOGLOBULIN VARIABLE REGION; LECTINS; LECTINS, C-TYPE; LIGANDS; MANNANS; MANNOSIDES; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MUTAGENESIS; OLIGOSACCHARIDES; PROTEIN CONFORMATION; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, CELL SURFACE;

HUMAN IMMUNODEFICIENCY VIRUS; HUMAN IMMUNODEFICIENCY VIRUS 1; RNA VIRUSES;

EID: 12444291017     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.1083182     Document Type: Article

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70. Supported by NIH Grants GM46192 (I.A.W.), AI33292 (D.R.B.), and a grant from IAVI (International Aids Vaccine Initiative) through the Neutralizing Antibody Consortium. We are grateful to the staff of the Stanford Synchrotron Radiation Laboratory (SSRL) Beamline 11-1 for assistance at the beamline; E. O. Saphire (The Scripps Research Institute) for the initial model of the carbohydrate on gp120; A. Heine (The Scripps Research Institute), R. Aguilar (The Scripps Research Institute), S. Church (The Scripps Research Institute) and B. Matthews (Oxford Glycobiology Institute, UK) for expert technical assistance; R. Lerner (The Scripps Research Institute), J. Binley (The Scripps Research Institute), and M. Crispin (Oxford Glycobiology Institute, UK) for helpful discussion and comments. This is publication 15508-MB from The Scripps Research Institute. Coordinates have been deposited in the Protein Data Bank (codes 1OM3, 1OP3, and 1OP5) and will be released immediately upon publication.