Optimisation of the purification process of a tumour-targeting antibody produced in N. benthamiana using vacuum-agroinfiltration

Transgenic Research

Volumn 19, Issue 6, 2010, Pages 1083-1097

  Lombardi, Raffaele ^a   Villani, Maria Elena ^a   Di Carli, Mariasole ^a   Brunetti, Patrizia ^a   Benvenuto, Eugenio ^a   Donini, Marcello ^a  

^a ENEA   (Italy)

Author keywords

Agroinfiltration; Antibody purification; Molecular farming; Plantibody; Proteolysis

Indexed keywords

CANCER ANTIBODY; ENDOTOXIN; IMMUNOGLOBULIN G; PLANTIBODY;

ARTICLE; BIOSYNTHESIS; COMPARATIVE STUDY; ENZYME LINKED IMMUNOSORBENT ASSAY; EVALUATION; GEL CHROMATOGRAPHY; GENE EXPRESSION; GENETICS; HUMAN; IMMUNOLOGY; ISOLATION AND PURIFICATION; METABOLISM; PILOT STUDY; PLANT LEAF; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ENGINEERING; RHIZOBIUM RADIOBACTER; SURFACE PLASMON RESONANCE; TOBACCO; TRANSGENIC PLANT; VACUUM; WESTERN BLOTTING;

ANTIBODIES, NEOPLASM; BLOTTING, WESTERN; CHROMATOGRAPHY, GEL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ENDOTOXINS; ENZYME-LINKED IMMUNOSORBENT ASSAY; GENE EXPRESSION; HUMANS; IMMUNOGLOBULIN G; PILOT PROJECTS; PLANT LEAVES; PLANTIBODIES; PLANTS, GENETICALLY MODIFIED; PROTEIN ENGINEERING; RHIZOBIUM RADIOBACTER; SURFACE PLASMON RESONANCE; TOBACCO; VACUUM;

NICOTIANA BENTHAMIANA;

EID: 78149470963     PISSN: 09628819     EISSN: None     Source Type: Journal    
DOI: 10.1007/s11248-010-9382-9     Document Type: Article

References (31)

1. Birch JR, Racher AJ (2006) Antibody production. Adv Drug Deliv Rev 58: 671-685.
2. Borsi L, Carnemolla B, Nicolò G, Spina B, Tanara G, Zardi L (1992) Expression of different tenascin isoforms in normal, hyperplastic and neoplastic human breast tissues. Int J Cancer 52: 688-692.
3. Daniell H, Sheatfield SJ, Wycoff K (2001) Medical molecular farming: production of antibodies, biopharmaceuticals and edible vaccines in plants. Trends Plant Sci 6: 219-226.
4. De Muynck B, Navarre C, Nizet Y, Stadlmann J, Boutry M (2009) Different subcellular localization and glycosylation for a functional antibody expressed in Nicotiana tabacum plants and suspension cells. Transgenic Res 18: 467-482.
5. Delannoy M, Alves G, Vertommen D, Ma J, Boutry M, Navarre C (2008) Identification of peptidases in Nicotiana tabacum leaf intercellular fluid. Proteomics 8: 2285-2298.
6. Drake PM, Barbi T, Sexton A, McGowan E, Stadlmann J, Navarre C, Paul MJ, Ma JK (2009) Development of rhizosecretion as a production system for recombinant proteins from hydroponic cultivated tobacco. FASEB J 23: 3581-3589.
7. Fischer R, Liao YC, Drossard J (1999) Affinity-purification of a TMV-specific recombinant full-size antibody from a transgenic tobacco suspension culture. J Immunol Methods 226: 1-10.
8. Gill SC, von Hippel PH (1989) Calculation of protein extinction coefficients from amino acid sequence data. Anal Biochem 182: 319-326.
9. Giritch A, Marillonnet S, Engler C, van Eldik G, Botterman J, Klimyuk V, Gleba Y (2006) Rapid high-yield expression of full-size IgG antibodies in plants coinfected with noncompeting viral vectors. Proc Natl Acad Sci 103: 14701-14706.
10. Hassan S, van Dolleweerd CJ, Ioakeimidis F, Keshavarz-Moore E, Ma JK (2008) Considerations for extraction of monoclonal antibodies targeted to different subcellular compartments in transgenic tobacco plants. Plant Biotechnol J 6: 733-748.
11. Hull AK, Criscuolo CJ, Mett V, Groen H, Steeman W, Westra H, Chapman G, Legutki B, Baillie L, Yusibov V (2005) Human-derived, plant-produced monoclonal antibody for the treatment of anthrax. Vaccine 23: 2082-2086.
12. Kathuria SR, Nath R, Pal R, Singh O, Fischer R, Lohiya NK, Talwar GP (2002) Functional recombinant antibodies against human chorionic gonadotropin expressed in plants. Curr Sci 82: 1452-1457.
13. Lohaus G, Pennewiss K, Sattelmacher B, Hussmann M, Hermann Muehling K (2001) Is the infiltration-centrifugation technique appropriate for the isolation of apoplastic fluid? A critical evaluation with different plant species. Physiol Plant 111: 457-465.
14. Marusic C, Nuttall J, Buriani G, Lico C, Lombardi R, Baschieri S, Benvenuto E, Frigerio L (2007) Expression, intracellular targeting and purification of HIV Nef variants in tobacco cells. BMC Biotechnol 7: 12.
15. Merril CR, Dunau ML, Goldman D (1981) A rapid sensitive silver stain for polypeptides in polyacrylamide gels. Anal Biochem 110: 201-207.
16. Negrouk V, Eisner G, Hi Lee, Han K, Taylor D, Wong HC (2005) Highly efficient transient expression of functional recombinant antibodies in lettuce. Plant Sci 169: 433-438.
17. Orzaez D, Mirabel S, Wieland WH, Granell A (2006) Agroinjection of tomato fruits. A tool for rapid functional analysis of transgenes directly in fruit. Plant Physiol 140: 3-11.
18. Platis D, Labrou NE (2006) Development of an aqueous two-phase partitioning system for fractionating therapeutic proteins from tobacco extract. J Chromatogr A 1128: 114-124.
19. Platis D, Labrou NE (2009) Application of a PEG/salt aqueous two-phase partition system for the recovery of monoclonal antibodies from unclarified transgenic tobacco extract. Biotechnol J 4: 1320-1327.
20. Platis D, Drossard J, Fischer R, Ma JK, Labrou NE (2008) New downstream processing strategy for the purification of monoclonal antibodies from transgenic tobacco plants. J Chromatogr A 1211: 80-89.
21. Rodríguez M, Ramírez NI, Ayala M, Freyre F, Pérez L, Triguero A, Mateo C, Selman-Housein G, Gavilondo JV, Pujol M (2005) Transient expression in tobacco leaves of an aglycosylated recombinant antibody against the epidermal growth factor receptor. Biotechnol Bioeng 89: 188-194.
22. Sainsbury F, Lavoie PO, D'Aoust MA, Vézina LP, Lomonossoff GP (2008) Expression of multiple proteins using full-length and deleted versions of cowpea mosaic virus RNA-2. Plant Biotechnol J 6: 82-92.
23. Sainsbury F, Thuenemann EC, Lomonossoff GP (2009) pEAQ: versatile expression vectors for easy and quick transient expression of heterologous proteins in plants. Plant Biotechnol J 7: 682-693.
24. Shukla AA, Hubbard B, Tressel T, Guhan S, Low D (2007) Downstream processing of monoclonal antibodies-application of platform approaches. J Chromatogr B 848: 28-39.
25. Strasser R, Stadlmann J, Schähs M, Stiegler G, Quendler H, Mach L, Glössl J, Weterings K, Pabst M, Steinkellner H (2008) Generation of glyco-engineered Nicotiana benthamiana for the production of monoclonal antibodies with a homogeneous human-like N-glycan structure. Plant Biotechnol J 6: 392-402.
26. Strasser R, Castilho A, Stadlmann J, Kunert R, Quendler H, Gattinger P, Jez J, Rademacher T, Altmann F, Mach L, Steinkellner H (2009) Improved virus neutralization by plant-produced anti-HIV antibodies with a homogeneous β1, 4-galactosylated N-glycan profile. J Biol Chem 284: 20479-20485.
27. Tavladoraki P, Girotti A, Donini M, Arias FJ, Mancini C, Morea V, Chiaraluce R, Consalvi V, Benvenuto E (1999) A single-chain antibody fragment is functionally expressed in the cytoplasm of both Escherichia coli and transgenic plants. Eur J Biochem 262: 617-624.
28. Valdés R, Gómez L, Padilla S, Brito J, Reyes B, Alvarez T, Mendoza O, Herrera O, Ferro W, Pujol M, Leal V, Linares M, Hevia Y, García C, Milá L, García O, Sánchez R, Acosta A, Geada D, Paez R, Luis Vega J, Borroto C (2003) Large-scale purification of an antibody directed against hepatitis B surface antigen from transgenic tobacco plants. Biochem Biophys Res Commun 308: 94-100.
29. Vaquero C, Sack M, Chandler J, Drossard J, Schuster F, Monecke M, Schillberg S, Fischer R (1999) Transient expression of a tumor-specific single-chain fragment and a chimeric antibody in tobacco leaves. Proc Natl Acad Sci 96: 11128-11133.
30. Vézina LP, Faye L, Lerouge P, D'Aoust MA, Marquet-Blouin E, Burel C, Lavoie PO, Bardor M, Gomord V (2009) Transient co-expression for fast and high-yield production of antibodies with human-like N-glycans in plants. Plant Biotechnol J 7: 442-455.
31. Villani ME, Morgun B, Brunetti P, Marusic C, Lombardi R, Pisoni I, Bacci C, Desiderio A, Benvenuto E, Donini M (2009) Plant pharming of a full-sized, tumour-targeting antibody using different expression strategies. Plant Biotechnol J 7: 59-72.