Multi-dimensional fractionation and characterization of crude protein mixtures: Toward establishment of a database of protein purification process development parameters

Biotechnology and Bioengineering

Volumn 109, Issue 12, 2012, Pages 3070-3083

  Nfor, Beckley K ^a   Ahamed, Tangir ^a   Pinkse, Martijn W H ^a   van der Wielen, Luuk A M ^a   Verhaert, Peter D E M ^a   van Dedem, Gijs W K ^a   Eppink, Michel H M ^b   van de Sandt, Emile J A X ^c   Ottens, Marcel ^a  

^a DELFT UNIVERSITY OF TECHNOLOGY   (Netherlands)

^b MSD   (Netherlands)

^c DSM RESOLVE   (Netherlands)

Author keywords

Chromatography; Crude mixture of proteins; Hybridoma cell culture supernatant; Monoclonal antibody; Process development parameters

Indexed keywords

CHROMATOGRAPHIC RESINS; CRUDE MIXTURES; CRUDE PROTEINS; CULTURE SUPERNATANT; DOWNSTREAM PROCESS; FAST ACQUISITION; GEL ELECTROPHORESIS; HIGH THROUGHPUT SCREENING; HYBRIDOMA CELLS; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; INVERSE METHODS; ION-EXCHANGE CHROMATOGRAPHY; ISOTHERM PARAMETERS; KNOWLEDGE-BASED APPROACH; MOBILE PHASE CONDITIONS; MOLECULAR PROPERTIES; MONOCLONAL ANTIBODY PURIFICATION; OPERATING CONDITION; PROCESS DEVELOPMENT; PROTEIN IDENTIFICATION; PROTEIN PURIFICATION PROCESS; PROTEIN SEPARATIONS; STATIONARY PHASIS;

CELL CULTURE; CHROMATOGRAPHY; DRUG PRODUCTS; ELECTROPHORESIS; INVERSE PROBLEMS; ISOTHERMS; KNOWLEDGE BASED SYSTEMS; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MONOCLONAL ANTIBODIES; OPTIMIZATION; PROTEINS; PURIFICATION; SIZE EXCLUSION CHROMATOGRAPHY;

PARAMETER ESTIMATION;

MONOCLONAL ANTIBODY;

ARTICLE; CHROMATOGRAPHY; GEL PERMEATION CHROMATOGRAPHY; HIGH THROUGHPUT SCREENING; HYBRIDOMA CELL CULTURE; HYDROPHOBIC INTERACTION CHROMATOGRAPHY; ION EXCHANGE CHROMATOGRAPHY; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; PH; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROCESS DEVELOPMENT; PROCESS OPTIMIZATION; PROTEIN PURIFICATION;

ANTIBODIES, MONOCLONAL; BIOTECHNOLOGY; CHROMATOGRAPHY; COMPLEX MIXTURES; DATABASES, FACTUAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HYBRIDOMAS; HYDROPHOBIC AND HYDROPHILIC INTERACTIONS; MODELS, BIOLOGICAL; PROTEINS; TANDEM MASS SPECTROMETRY;

EID: 84868157621     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24576     Document Type: Article

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