Glycosylation analysis of an aggregated antibody produced by Chinese hamster ovary cells in bioreactor culture

Journal of Bioscience and Bioengineering

Volumn 117, Issue 5, 2014, Pages 639-644

  Onitsuka, Masayoshi ^a   Kawaguchi, Akira ^b   Asano, Ryutaro ^c   Kumagai, Izumi ^c   Honda, Kohsuke ^b   Ohtake, Hisao ^b   Omasa, Takeshi ^a,b  

^a UNIVERSITY OF TOKUSHIMA   (Japan)

^b OSAKA UNIVERSITY   (Japan)

^c TOHOKU UNIVERSITY   (Japan)

Author keywords

Antibody aggregation; Antibody production; Bispecific diabody; Cell culture; Chinese hamster ovary cell; Glycosylation heterogeneity

Indexed keywords

ANTIBODIES; BIOREACTORS; CELL CULTURE; CELLS; DIMERS; ESTERIFICATION; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

ANTIBODY AGGREGATION; ANTIBODY PRODUCTION; BIOLOGICAL FUNCTIONS; BIOREACTOR CULTURES; BISPECIFIC DIABODY; CHINESE HAMSTER OVARY CELLS; LARGE AGGREGATES; THERAPEUTIC ANTIBODIES;

AGGREGATES;

ANTIBODY; ASPARAGINE LINKED OLIGOSACCHARIDE; DIMER; FUCOSE; GALACTOSE;

ANIMAL CELL; ANIMAL CELL CULTURE; ARTICLE; BINDING ASSAY; BIOREACTOR; CARBOHYDRATE ANALYSIS; CELL VIABILITY; CHO CELL; CIRCULAR DICHROISM; DEGLYCOSYLATION; GEL PERMEATION CHROMATOGRAPHY; GLYCOSYLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; LECTIN BINDING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN SECONDARY STRUCTURE; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY;

CRICETULUS GRISEUS;

ANTIBODY AGGREGATION; ANTIBODY PRODUCTION; BISPECIFIC DIABODY; CELL CULTURE; CHINESE HAMSTER OVARY CELL; GLYCOSYLATION HETEROGENEITY;

ANIMALS; ANTIBODIES, BISPECIFIC; BIOREACTORS; CELL CULTURE TECHNIQUES; CHO CELLS; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; CRICETINAE; CRICETULUS; FUCOSE; GALACTOSE; GLYCOSYLATION; LECTINS; OLIGOSACCHARIDES; PROTEIN BINDING; PROTEIN MULTIMERIZATION; PROTEIN STABILITY; PROTEIN STRUCTURE, QUATERNARY;

EID: 84897065464     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2013.11.001     Document Type: Article

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