Heterologous expression of mycobacterial Esx complexes in Escherichia coli for structural studies is facilitated by the use of maltose binding protein fusions

PLoS ONE

Volumn 8, Issue 11, 2013, Pages

  Arbing, Mark A ^a   Chan, Sum ^a   Harris, Liam ^a   Kuo, Emmeline ^a   Zhou, Tina T ^a   Ahn, Christine J ^a   Nguyen, Lin ^a   He, Qixin ^a   Lu, Jamie ^a   Menchavez, Phuong T ^a   Shin, Annie ^a   Holton, Thomas ^a   Sawaya, Michael R ^a   Cascio, Duilio ^a   Eisenberg, David ^a,b,c  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

^c UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CULTURE FILTRATE PROTEIN 10; HEXAHISTIDINE; MALTOSE BINDING PROTEIN; BACTERIAL PROTEIN; HYBRID PROTEIN;

AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL STRAIN; BACTERIAL VIRULENCE; BIOCHEMISTRY; COMPARATIVE STUDY; CRYSTAL STRUCTURE; ESCHERICHIA COLI; HETEROLOGOUS EXPRESSION; MYCOBACTERIUM ABSCESSUS; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; NONHUMAN; PROTEIN CLEAVAGE; PROTEIN EXPRESSION; PROTEIN PURIFICATION; SOLUBILITY; AMINO ACID SEQUENCE; CHEMICAL STRUCTURE; CHEMISTRY; GENE EXPRESSION; GENE VECTOR; GENETIC ENGINEERING; GENETICS; MOLECULAR GENETICS; MYCOBACTERIUM; PROCEDURES; PROTEIN SECONDARY STRUCTURE; X RAY CRYSTALLOGRAPHY;

AMINO ACID SEQUENCE; BACTERIAL PROTEINS; CRYSTALLOGRAPHY, X-RAY; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC VECTORS; MALTOSE-BINDING PROTEINS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; MYCOBACTERIUM; PROTEIN STRUCTURE, SECONDARY; RECOMBINANT FUSION PROTEINS;

EID: 84896698543     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0081753     Document Type: Article

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