Enhancing the Solubility of Recombinant Proteins in Escherichia coli by Using Hexahistidine-Tagged Maltose-Binding Protein as a Fusion Partner

Methods in Molecular Biology

Volumn 705, Issue , 2011, Pages 259-274

  Sun, Ping ^a   Tropea, Joseph E ^a   Waugh, David S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

Gateway cloning; His tag; His6 MBP; Maltose binding protein; MBP; solubility enhancer; TEV protease; tobacco etch virus protease

Indexed keywords

ESCHERICHIA COLI PROTEIN; HISTIDINE; HYBRID PROTEIN; MALTOSE BINDING PROTEIN; POLYHISTIDINE;

AFFINITY CHROMATOGRAPHY; BIOSYNTHESIS; ESCHERICHIA COLI; GENETICS; METABOLISM; METHODOLOGY; REVIEW; SOLUBILITY;

CHROMATOGRAPHY, AFFINITY; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; HISTIDINE; MALTOSE-BINDING PROTEINS; RECOMBINANT FUSION PROTEINS; SOLUBILITY;

ESCHERICHIA COLI; NICOTIANA TABACUM; TOBACCO ETCH VIRUS;

EID: 79952476311     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61737-967-3_16     Document Type: Chapter

References (18)

1. Waugh, D. S. (2005) Making the most of affinity tags. Trends Biotechnol 23, 316–320.
2. Esposito, D., Chatterjee, D. K. (2006) Enhancement of soluble protein expression through the use of fusion tags. Curr Opin Biotechnol 17, 353–358.
3. Kapust, R. B., Waugh, D. S. (1999) Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused. Protein Sci 8, 1668–1674.
4. Fox, J. D., Routzahn, K. M., Bucher, M. H., Waugh, D. S. (2003) Maltodextrin-binding proteins from diverse bacteria and archaea are potent solubility enhancers. FEBS Lett 537, 53–57.
5. Lichty, J. J., Malecki, J. L., Agnew, H. D., Michelson-Horowitz, D. J., Tan, S. (2005) Comparison of affinity tags for protein purification. Protein Expr Purif 41, 98–105.
6. Pryor, K. D., Leiting, B. (1997) High-level expression of soluble protein in Escherichia coli using a His6-tag and maltose-binding-protein double-affinity fusion system. Protein Expr Purif 10, 309–319.
7. Routzahn, K. M., Waugh, D. S. (2002) Differential effects of supplementary affinity tags on the solubility of MBP fusion proteins. J Struct Funct Genomics 2, 83–92.
8. Nallamsetty, S., Austin, B. P., Penrose, K. J., Waugh, D. S. (2005) Gateway vectors for the production of combinatorially-tagged His6-MBP fusion proteins in the cytoplasm and periplasm of Escherichia coli. Protein Sci 14, 2964–2971.
9. Alexandrov, A., Dutta, K., Pascal, S. M. (2001) MBP fusion protein with a viral protease cleavage site: one-step cleav-age/purification of insoluble proteins. Biotechniques 30, 1194–1198.
10. Riggs, P. (2001) Expression and purification of maltose-binding protein fusions. Curr Protoc Mol Biol Chapter 16, Unit16 6.
11. Fox, J. D., Waugh, D. S. (2003) Maltose-binding protein as a solubility enhancer. Methods Mol Biol 205, 99–117.
12. Cabrita, L. D., Dai, W., Bottomley, S. P. (2006) A family of E. coli expression vectors for laboratory scale and high throughput soluble protein production. BMC Biotechnol 6, 12.
13. Donnelly, M. I., Zhou, M., Millard, C. S., Clancy, S., Stols, L., Eschenfeldt, W. H., Collart, F. R., Joachimiak, A. (2006) An expression vector tailored for large-scale, high-throughput purification of recombinant proteins. Protein Expr Purif 47, 446–454.
14. Jeon, W. B., Aceti, D. J., Bingman, C. A., Vojtik, F. C., Olson, A. C., Ellefson, J. M., McCombs, J. E., Sreenath, H. K., Blom-mel, P. G., Seder, K. D., Burns, B. T., Geetha, H. V., Harms, A. C., Sabat, G., Suss-man, M. R., Fox, B. G., Phillips, G. N., Jr. (2005) High-throughput purification and quality assurance of Arabidopsis thaliana proteins for eukaryotic structural genomics. J Struct Funct Genomics 6, 143–147.
15. Nallamsetty, S., Waugh, D. S. (2007) A generic protocol for the expression and purification of recombinant proteins in Escherichia coli using a combinatorial His6-maltose binding protein fusion tag. Nat Protoc 2, 383–391.
16. Tropea, J. E., Cherry, S., Nallamsetty, S., Bignon, C., Waugh, D. S. (2007) A generic method for the production of recombinant proteins in Escherichia coli using a dual hexahistidine-maltose-binding protein affinity tag. Methods Mol Biol 363, 1–19.
17. Kapust, R. B., Waugh, D. S. (2000) Controlled intracellular processing of fusion proteins by TEV protease. Protein Expr Purif 19, 312–318.
18. Sambrook, J., Russell, D. W. (2001) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.