Efficient site-specific processing of fusion proteins by tobacco vein mottling virus protease in vivo and in vitro

Protein Expression and Purification

Volumn 38, Issue 1, 2004, Pages 108-115

  Nallamsetty, Sreedevi ^a   Kapust, Rachel B ^a   Tözsér, József ^b   Cherry, Scott ^a   Tropea, Joseph E ^a   Copeland, Terry D ^a   Waugh, David S ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

^b UNIVERSITY OF DEBRECEN   (Hungary)

Author keywords

Affinity tag; Protease; TEV protease; Tobacco etch virus; Tobacco vein mottling virus; TVMV protease

Indexed keywords

HYBRID PROTEIN; MATRIX PROTEIN; PROTEINASE; SODIUM CHLORIDE; TEV PROTEASE; TVMV 34K PROTEIN, TOBACCO VEIN MOTTLING VIRUS;

AMINO ACID SEQUENCE; ARTICLE; ENZYME SPECIFICITY; GENE VECTOR; ISOLATION AND PURIFICATION; KINETICS; METABOLISM; MOLECULAR GENETICS; PLASMID; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; TEMPERATURE;

AMINO ACID SEQUENCE; ENDOPEPTIDASES; GENETIC VECTORS; KINETICS; MOLECULAR SEQUENCE DATA; PLASMIDS; RECOMBINANT FUSION PROTEINS; RESTRICTION MAPPING; SEQUENCE ALIGNMENT; SODIUM CHLORIDE; SUBSTRATE SPECIFICITY; TEMPERATURE; VIRAL MATRIX PROTEINS;

NICOTIANA TABACUM; TOBACCO ETCH VIRUS; TOBACCO VEIN MOTTLING VIRUS; TRIXIS;

EID: 5344232818     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2004.08.016     Document Type: Article

References (25)

1. C.V. Maina, P.D. Riggs, A.G. Grandea, E.E. Slatko, L.S. Moran, J.A. Tagliamonte, L.A. McReynolds, and C. Guan A vector to express and purify foreign proteins in Escherichia coli by fusion to and separation from, maltose binding protein Gene 74 1988 365 373
2. D.B. Smith, and K.S. Johnson Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase Gene 67 1988 31 40
3. F. Baneyx Recombinant protein expression in Escherichia coli Curr. Opin. Biotechnol. 10 1999 411 421
4. J. Nilsson, S. Stahl, J. Lundeberg, M. Uhlen, and P.A. Nygren Affinity fusion strategies for detection, purification and immobilization of recombinant proteins Protein Expr. Purif. 11 1997 1 16
5. D.P. Humphreys, L.M. King, S.M. West, A.P. Chapman, M. Sehdev, M.W. Redden, D.J. Glover, B.J. Smith, and P.E. Stephens Improved efficiency of site-specific copper(II) ion-catalysed protein cleavage effected by mutagenesis of cleavage site Protein Eng. 13 1999 201 216
6. T.R. Butt, S. Jonnalagadda, B.P. Monia, E.J. Sternberg, J.A. Marsh, J.M. Stadel, D.J. Ecker, and S.T. Crooke Ubiquitin fusion augments the yield of cloned gene products in Escherichia coli Proc. Natl. Acad. Sci. USA 86 1989 2540 2544
7. A. Jacquet, V. Daminet, M. Haumont, L. Garcia, S. Chaudoir, A. Bollen, and R. Biemans Expression of a recombinant Toxoplasma gondii ROP2 fragment as a fusion protein in bacteria circumvents insolubility and proteolytic degradation Protein Expr. Purif. 17 1999 392 400
8. R.B. Kapust, and D.S. Waugh Escherichia coli maltose-binding protein is uncommonly effective at promoting the solubility of polypeptides to which it is fused Protein Sci. 8 1999 1668 1674
9. L. Rolf, and B. Hermann Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements Nucleic Acids Res. 25 1997 1203 1210
10. L.M. Babe, and C.S. Craik Viral proteases: evolution of diverse structural motifs to optimize function Cell 91 1997 421 427
11. R.C. Stevens Design of high-throughput methods of protein production for structural biology Structure Fold Des. 8 2000 R177 R185
12. R.B. Kapust, J. Tozser, T.D. Copeland, and D.S. Waugh The P1' specificity of tobacco etch virus protease Biochem. Biophys. Res. Commun. 294 2002 949 955
13. D.T. Parks, D.E. Howard, T.J. Wolpert, J.D. Arp, and G.W. Dougherty Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occurring truncated proteinase form Virology 210 1995 194 201
14. R.B. Kapust, J. Tozser, J.D. Fox, D.E. Anderson, S. Cherry, T.D. Copeland, and D.S. Waugh Tobacco etch virus protease: mechanism of autolysis and rational design of stable mutants with wild-type catalytic proficiency Protein Eng. 12 2001 993 1000
15. L.J. Lucast, R.T. Batey, and J.A. Doudna Large-scale purification of a stable form of recombinant tobacco etch virus protease Biotechniques 30 2001 544 546
16. D.C. Hwang, D.H. Kim, J.S. Lee, B.H. Kang, J. Han, W. Kim, B.D. Song, and K.Y. Choi Characterization of active-site residues of the NIa protease from tobacco vein mottling virus Mol. Cells 31 2000 505 511
17. D.M. Hoover, and J. Lubkowski DNA Works: an automated method for designing oligonucleotides for PCR-based gene synthesis Nucleic Acids Res. 30 2002 e43
18. J.D. Fox, and D.S. Waugh Maltose-binding protein as a solubility enhancer Methods Mol. Biol. 205 2003 99 117
19. R.B. Kapust, and D.S. Waugh Controlled intracellular processing of fusion proteins by TEV protease Protein Expr. Purif. 19 2000 312 318
20. J.H. Miller Experimental in Molecular Genetics 1972 Cold Spring Harbor Laboratory Cold Spring Harbor, NY
21. J. Sambrook, E.F. Fritsch, and T. Maniatis Molecular cloning: A Laboratory Manual Second ed. 1989 Cold Spring Harbor Laboratory Press Cold Spring Harbor, NY
22. P. Boross, P. Bagossi, T.D. Copeland, S. Oroszlan, L.M. Louis, and J. Tözsér Effect of substrate residues on the P2' preference of retroviral proteinases Eur. J. Biochem. 264 1999 921 929
23. H.Y. Yoon, D.C. Hwang, K.Y. Choi, and B.D. Song Proteolytic processing of oligopeptides containing the target sequences by the recombinant tobacco vein mottling virus NIa proteinase Mol. Cells 10 2000 213 219
24. G. Forsberg, B. Baastrup, H. Rondahl, E. Holmgren, G. Pohl, M. Hartmanis, and M. Lake An evaluation of different enzymatic cleavage methods for recombinant fusion proteins, applied on des(1-3)insulin-like growth factor I J. Protein Chem. 11 1992 201 211
25. T.D. Parks, K.K. Leuther, E.D. Howard, S.A. Johnston, and W.G. Dougherty Release of proteins and peptides from fusion proteins using a recombinant plant virus proteinase Anal. Biochem. 216 1994 413 417