A new Gateway® vector and expression protocol for fast and efficient recombinant protein expression in Mycobacterium smegmatis

Protein Expression and Purification

Volumn 57, Issue 1, 2008, Pages 81-87

  Goldstone, Rachael M ^a   Moreland, Nicole J ^a   Bashiri, Ghader ^a   Baker, Edward N ^a   Shaun Lott, J ^a  

^a UNIVERSITY OF AUCKLAND   (New Zealand)

Author keywords

Autoinduction media; Gateway; Inclusion bodies; Mycobacterium smegmatis; Mycobacterium tuberculosis; Recombinant protein expression

Indexed keywords

ESCHERICHIA COLI; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS;

BACTERIAL PROTEIN; HISTIDINE; RECOMBINANT PROTEIN;

ARTICLE; BACTERIUM TRANSFORMATION; CELL INCLUSION; CHEMISTRY; CULTURE MEDIUM; DNA SEQUENCE; ESCHERICHIA COLI; GENE EXPRESSION; GENE VECTOR; GENETICS; METABOLISM; MICROBIOLOGICAL EXAMINATION; MOLECULAR CLONING; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; OPEN READING FRAME; PROMOTER REGION; REPRODUCIBILITY; RESTRICTION MAPPING; SOLUBILITY; TIME;

BACTERIAL PROTEINS; CLONING, MOLECULAR; CULTURE MEDIA; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HISTIDINE; INCLUSION BODIES; MICROBIOLOGICAL TECHNIQUES; MYCOBACTERIUM SMEGMATIS; MYCOBACTERIUM TUBERCULOSIS; OPEN READING FRAMES; PROMOTER REGIONS (GENETICS); RECOMBINANT PROTEINS; REPRODUCIBILITY OF RESULTS; RESTRICTION MAPPING; SEQUENCE ANALYSIS, DNA; SOLUBILITY; TIME FACTORS; TRANSFORMATION, BACTERIAL;

EID: 36249015337     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2007.08.015     Document Type: Article

References (12)

1. Cowieson N.P., Wensley B., Listwan P., Hume D.A., Kobe B., and Martin J.L. An automatable screen for the rapid identification of proteins amenable to refolding. Proteomics 6 (2006) 1750-1757
2. Moreland N., Ashton R., Baker H.M., Ivanovic I., Patterson S., Arcus V.L., Baker E.N., and Lott J.S. A flexible and economical medium-throughput strategy for protein production and crystallization. Acta Crystallographica Section D: Biological Crystallography 61 (2005) 1378-1385
3. Vincentelli R., Canaan S., Campanacci V., Valencia C., Maurin D., Frassinetti F., Scappucini-Calvo L., Bourne Y., Cambillau C., and Bignon C. High-throughput automated refolding screening of inclusion bodies. Protein Science 13 (2004) 2782-2792
4. Snapper S.B., Melton R.E., Mustafa S., Kieser T., and Jacobs Jr W.R. Isolation and characterization of efficient plasmid transformation mutants of Mycobacterium smegmatis. Molecular Microbiology 4 (1990) 1911-1919
5. Bashiri G., Squire C.J., Baker E.N., and Moreland N.J. Expression, purification and crystallization of native and selenomethionine labeled Mycobacterium tuberculosis FGD1 (Rv0407) using a Mycobacterium smegmatis expression system. Protein Expression and Purification 54 (2007) 38-44
6. Delogu G., Bua A., Pusceddu C., Parra M., Fadda G., Brennan M.J., and Zanetti S. Expression and purification of recombinant methylated HBHA in Mycobacterium smegmatis. FEMS Microbiology Letters 239 (2004) 33-39
7. Gutierrez-Lugo M.T., Newton G.L., Fahey R.C., and Bewley C.A. Cloning, expression and rapid purification of active recombinant mycothiol ligase as B1 immunoglobulin binding domain of streptococcal protein G, glutathione-S-transferase and maltose binding protein fusion proteins in Mycobacterium smegmatis. Protein Expression and Purification 50 (2006) 128-136
8. Studier F.W. Protein production by auto-induction in high-density shaking cultures. Protein Expression and Purification 41 (2005) 207-234
9. Gasteiger E., Hoogland C., Gattiker A., Duvaud S., Wilkins M.R., Appel R.D., and Bairoch A. Protein identification and analysis tools on the ExPASy server. In: Walker J.M. (Ed). The Proteomics Protocols Handbook (2005), Humana Press 571-607
10. Listwan P., Cowieson N., Kurz M., Hume D.A., Martin J.L., and Kobe B. Modification of recombinatorial cloning for small affinity tag fusion protein construct generation. Analytical Biochemistry 346 (2005) 327-329
11. Qamra R., Srinivas V., and Mande S.C. Mycobacterium tuberculosis GroEL homologues unusually exist as lower oligomers and retain the ability to suppress aggregation of substrate proteins. Journal of Molecular Biology 342 (2004) 605-617
12. Saibil H.R., and Ranson N.A. The chaperonin folding machine. Trends in Biochemical Sciences 27 (2002) 627-632