Structural insights into the specificity of Xyn10B from paenibacillus barcinonensis and its improved stability by forced protein evolution

Journal of Biological Chemistry

Volumn 285, Issue 4, 2010, Pages 2721-2733

  Gallardo, Óscar ^a   Javier Pastor, F I ^a   Polaina, Julio ^b   Diaz, Pilar ^a   Łysek, Robert ^c   Vogel, Pierre ^c   Isorna, Pablo ^d   González, Beatriz ^d   Sanz Aparicio, Julia ^d  

^a UNIVERSITY OF BARCELONA   (Spain)

^b INSTITUTO DE AGROQUÍMICA Y TECNOLOGÍA DE ALIMENTOS CSIC   (Spain)

^c EPFL   (Switzerland)

^d INSTITUTO DE QUÍMICA FÍSICA ROCASOLANO   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

C TERMINUS; CLOSE PROXIMITY; COCRYSTALLIZATION; COMPACT STRUCTURES; CRYSTALLIZATION MEDIUM; DOUBLE MUTANTS; ENZYME FOLD; GLUCURONIC ACIDS; GLYCOSYL HYDROLASES; HYDROPHOBIC PACKING; INACTIVE ENZYMES; OPEN CAVITY; PROTEIN EVOLUTION; SECONDARY STRUCTURE ELEMENTS; SOIL BACTERIUM; STRUCTURAL INSIGHTS; THERMAL STABILITY; WILD TYPES; XYLANASE ACTIVITY; XYLANASES; XYLO- OLIGOSACCHARIDES;

CATALYSTS; CRYSTALLIZATION; ENZYMES; GLUCOSE; HEAT RESISTANCE; PHOTODEGRADATION; THERMOGRAVIMETRIC ANALYSIS;

CRYSTAL STRUCTURE;

BACTERIAL ENZYME; GLUCURONIC ACID; GLUTAMIC ACID; HISTIDINE; LEUCINE; MUTANT PROTEIN; OLIGOSACCHARIDE; PROTEIN GH10; PROTEIN XYN10B; SERINE; UNCLASSIFIED DRUG; XYLAN ENDO 1,3 BETA XYLOSIDASE; ENDO 1,4 BETA XYLANASE; TEPROTIDE; XYLAN;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; CRYSTALLIZATION; ENZYME ACTIVITY; ENZYME INACTIVATION; MOLECULAR DOCKING; MOLECULAR EVOLUTION; NONHUMAN; PAENIBACILLUS; PAENIBACILLUS BARCINONENSIS; PRIORITY JOURNAL; PROTEIN MOTIF; PROTEIN SECONDARY STRUCTURE; PROTEIN STABILITY; THERMOSTABILITY; WILD TYPE; CATALYSIS; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; MICROBIOLOGY; PROTEIN FOLDING; PROTEIN TERTIARY STRUCTURE; SITE DIRECTED MUTAGENESIS; X RAY CRYSTALLOGRAPHY;

BACTERIA (MICROORGANISMS); PAENIBACILLUS BARCINONENSIS;

CATALYSIS; CATALYTIC DOMAIN; CRYSTALLOGRAPHY, X-RAY; ENDO-1,4-BETA XYLANASES; EVOLUTION, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PAENIBACILLUS; PROTEIN FOLDING; PROTEIN STRUCTURE, SECONDARY; PROTEIN STRUCTURE, TERTIARY; SOIL MICROBIOLOGY; SUBSTRATE SPECIFICITY; TEPROTIDE; XYLANS;

EID: 77449110968     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M109.064394     Document Type: Article

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