Engineering enzyme stability and resistance to an organic cosolvent by modification of residues in the access tunnel

Angewandte Chemie - International Edition

Volumn 52, Issue 7, 2013, Pages 1959-1963

  Koudelakova, Tana ^a   Chaloupkova, Radka ^a   Brezovsky, Jan ^a   Prokop, Zbynek ^a   Sebestova, Eva ^a   Hesseler, Martin ^b   Khabiri, Morteza ^c   Plevaka, Maryia ^d   Kulik, Daryna ^d   Kuta Smatanova, Ivana ^c,d   Rezacova, Pavlina ^e   Ettrich, Rudiger ^c   Bornscheuer, Uwe T ^b   Damborsky, Jiri ^a  

^a MASARYK UNIVERSITY   (Czech Republic)

^b UNIVERSITY OF GREIFSWALD   (Germany)

^c INSTITUTE OF MATHEMATICS   (Czech Republic)

^d UNIVERSITY OF SOUTH BOHEMIA   (Czech Republic)

^e INSTITUTE OF MOLECULAR GENETICS   (Czech Republic)

Author keywords

directed evolution; enzyme catalysis; enzymes; protein engineering; protein stability

Indexed keywords

COSOLVENT MOLECULES; COSOLVENTS; DIRECTED EVOLUTION; ENZYME CATALYSIS; ENZYME STABILITY; KEY DETERMINANTS; PROTEIN CRYSTALLOGRAPHY; PROTEIN ENGINEERING; PROTEIN STABILITY;

CRYSTALLOGRAPHY; DIMETHYL SULFOXIDE; GENETIC ENGINEERING; MOLECULAR DYNAMICS; PROTEINS;

ENZYMES;

DIMETHYL SULFOXIDE; ENZYME; SOLVENT;

ARTICLE; BIOCATALYSIS; CHEMISTRY; ENZYME STABILITY; KINETICS; METABOLISM; MOLECULAR DYNAMICS; MOLECULAR EVOLUTION; PROTEIN ENGINEERING;

BIOCATALYSIS; DIMETHYL SULFOXIDE; ENZYME STABILITY; ENZYMES; EVOLUTION, MOLECULAR; KINETICS; MOLECULAR DYNAMICS SIMULATION; PROTEIN ENGINEERING; SOLVENTS;

EID: 84873532872     PISSN: 14337851     EISSN: 15213773     Source Type: Journal    
DOI: 10.1002/anie.201206708     Document Type: Article

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