High-throughput screening of B factor saturation mutated Rhizomucor miehei lipase thermostability based on synthetic reaction

Enzyme and Microbial Technology

Volumn 50, Issue 6-7, 2012, Pages 325-330

  Zhang, Jun Hui ^a   Lin, Ying ^a   Sun, Yu Fei ^a,b   Ye, Yan Rui ^a   Zheng, Sui Ping ^a   Han, Shuang Yan ^a  

^a SOUTH CHINA UNIVERSITY OF TECHNOLOGY   (China)

^b GUANGXI UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

Author keywords

High throughput screening; PH indicator; Rhizomucor miehei lipase; Synthetic activity; Thermostability; Yeast surface display

Indexed keywords

HIGH-THROUGHPUT SCREENING; PH INDICATOR; RHIZOMUCOR MIEHEI LIPASE; SURFACE DISPLAYS; THERMOSTABILITY;

AMINO ACIDS; ETHANOL; ISOMERS; PH; YEAST;

LIPASES;

ALCOHOL; FUNGAL ENZYME; METHYL RED; OCTANOIC ACID; TRIACYLGLYCEROL LIPASE;

AMINO ACID SUBSTITUTION; ANALYTIC METHOD; ARTICLE; CLONE; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; ENZYME MECHANISM; ENZYME STABILITY; ENZYME SUBSTRATE; ESTERIFICATION; HIGH THROUGHPUT SCREENING; MUTANT; NONHUMAN; PH; REACTION ANALYSIS; RHIZOMUCOR; RHIZOMUCOR MIEHEI; THERMOSTABILITY; WILD TYPE;

BIOTECHNOLOGY; CULTURE MEDIA; ENZYME STABILITY; ENZYMES, IMMOBILIZED; ESTERIFICATION; ETHANOL; HIGH-THROUGHPUT SCREENING ASSAYS; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; LIPASE; MUTATION; OCTANOIC ACIDS; RHIZOMUCOR;

RHIZOMUCOR MIEHEI;

EID: 84859764493     PISSN: 01410229     EISSN: 18790909     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2012.03.002     Document Type: Article

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