Development of thermostable lipase B from Candida antarctica (CalB) through in silico design employing B-factor and RosettaDesign

Enzyme and Microbial Technology

Volumn 47, Issue 1-2, 2010, Pages 1-5

  Kim, Hyun Sook ^a   Le, Quang Anh Tuan ^a   Kim, Yong Hwan ^a  

^a Kwangwoon University   (South Korea)

Author keywords

B factor; Candida antarctica lipase B; Rational design; RosettaDesign; Thermostability

Indexed keywords

ANALYSIS TOOLS; ATOMIC DISPLACEMENT PARAMETERS; CANDIDA ANTARCTICA LIPASE B; COMPUTATIONAL MODELING; ELEVATED REACTIONS; FLUORIMETRY; HIGHER TEMPERATURES; IN-SILICO; LIPASE B FROM CANDIDA ANTARCTICA; MELTING TEMPERATURES; ORGANIC SYNTHESIS; RATIONAL DESIGN; RESIDUAL ACTIVITY; THERMAL STABILITY; WILD TYPES; X RAY DATA;

AMINO ACIDS; ENANTIOSELECTIVITY; ENZYMES; ISOMERS; ORGANIC ACIDS; REACTION RATES; SYNTHESIS (CHEMICAL); THERMODYNAMIC STABILITY; YEAST;

DESIGN;

ENZYME VARIANT; LIPASE B;

ARTICLE; CANDIDA ANTARCTICA; COMPUTER MODEL; CONTROLLED STUDY; DIFFERENTIAL SCANNING CALORIMETRY; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STABILITY; FUNGUS MUTANT; GENE EXPRESSION; MELTING POINT; MUTAGENESIS; NONHUMAN; THERMODYNAMICS; WILD TYPE; X RAY;

CANDIDA ANTARCTICA;

EID: 77953621626     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2010.04.003     Document Type: Article

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