High-content, high-throughput screening for the identification of cytotoxic compounds based on cell morphology and cell proliferation markers

PLoS ONE

Volumn 9, Issue 2, 2014, Pages

  Martin, Heather L ^a   Adams, Matthew ^a   Higgins, Julie ^a   Bond, Jacquelyn ^a   Morrison, Ewan E ^a   Bell, Sandra M ^a   Warriner, Stuart ^a   Nelson, Adam ^a   Tomlinson, Darren C ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

4',6 DIAMIDINO 2 PHENYLINDOLE; CELL MARKER; DOXORUBICIN; HISTONE H3; NOCODAZOLE; PACLITAXEL;

ALGORITHM; APOPTOSIS; ARTICLE; CELL ASSAY; CELL PROLIFERATION; CELL SHAPE; CELL STRUCTURE; CONCENTRATION RESPONSE; CONTROLLED STUDY; CYTOSOL; CYTOTOXICITY TEST; DRUG DETERMINATION; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; LONG TERM EXPOSURE; MITOSIS; MOLECULAR LIBRARY; PHENOTYPE;

ALGORITHMS; BIOLOGICAL PRODUCTS; CELL LINE; CELL PROLIFERATION; CELL SHAPE; DRUG EVALUATION, PRECLINICAL; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; SMALL MOLECULE LIBRARIES; SOFTWARE;

EID: 84895535497     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0088338     Document Type: Article

References (28)

1. Paul SM, Mytelka DS, Dunwiddie CT, Persinger CC, Munos BH, et al. (2010) How to improve R&D productivity: the pharmaceutical industry's grand challenge. Nat Rev Drug Discov 9: 203-214.
2. Slater K (2001) Cytotoxicity tests for high-throughput drug discovery. Curr Opin Biotechnol 12: 70-74.
3. Haney SA, LaPan P, Pan J, Zhang J (2006) High-content screening moves to the front of the line. Drug Discov Today 11: 889-894. (Pubitemid 44403188)
4. Korn K, Krausz E (2007) Cell-based high-content screening of small-molecule libraries. Current Opinion in Chemical Biology 11: 503-510. (Pubitemid 47589005)
5. O'Brien PJ, Irwin W, Diaz D, Howard-Cofield E, Krejsa CM, et al. (2006) High concordance of drug-induced human hepatotoxicity with in vitro cytotoxicity measured in a novel cell-based model using high content screening. Arch Toxicol 80: 580-604. (Pubitemid 44214526)
6. Zanella F, Lorens JB, Link W (2010) High content screening: seeing is believing. Trends in Biotechnology 28: 237-245.
7. Persson M, Løye AF, Mow T, Hornberg JJ (2013) A high content screening assay to predict human drug-induced liver injury during drug discovery. Journal of Pharmacological and Toxicological Methods 68: 302-313.
8. Moffat J, Grueneberg DA, Yang X, Kim SY, Kloepfer AM, et al. (2006) A Lentiviral RNAi Library for Human and Mouse Genes Applied to an Arrayed Viral High-Content Screen. Cell 124: 1283-1298.
9. Murrison S, Maurya SK, Einzinger C, McKeever-Abbas B, Warriner S, et al. (2011) Synthesis of Skeletally Diverse Alkaloid-Like Small Molecules. European Journal of Organic Chemistry 2011: 2354-2359.
10. O'Leary-Steele C, Pedersen PJ, James T, Lanyon-Hogg T, Leach S, et al. (2010) Synthesis of Small Molecules with High Scaffold Diversity: Exploitation of Metathesis Cascades in Combination with Inter- and Intramolecular Diels-Alder Reactions. Chemistry - A European Journal 16: 9563-9571.
11. Morton D, Leach S, Cordier C, Warriner S, Nelson A (2009) Synthesis of Natural-Product-Like Molecules with Over Eighty Distinct Scaffolds. Ange-wandte Chemie International Edition 48: 104-109.
12. Bray MA CA (2012) Advanced Assay Development Guidelines for Image-Based High Content Screening and Analysis. In: Sittampalam GS G-EN, Arkin M, et al, editor. http://www.ncbi.nlm.nih.gov/books/NBK126174/.
13. Zhang J-H, Chung TDY, Oldenburg KR (1999) A Simple Statistical Parameter for Use in Evaluation and Validation of High Throughput Screening Assays. Journal of Biomolecular Screening 4: 67-73. (Pubitemid 29278954)
14. Ghosh RN, DeBiasio R, Hudson CC, Ramer ER, Cowan CL, et al. (2005) Quantitative Cell-Based High-Content Screening for Vasopressin Receptor Agonists Using TransfluorHTechnology. Journal of Biomolecular Screening 10: 476-484. (Pubitemid 41233226)
15. Mukherji M, Bell R, Supekova L, Wang Y, Orth AP, et al. (2006) Genome-wide functional analysis of human cell-cycle regulators. Proceedings of the National Academy of Sciences 103: 14819-14824. (Pubitemid 44527812)
16. Torres-Guzmán R, Chu S, Velasco JA, Lallena MJ (2013) Multiparametric Cell-Based Assay for the Evaluation of Transcription Inhibition by High-Content Imaging. Journal of Biomolecular Screening 18: 556-566.
17. Adams M, Cookson VJ, Higgins J, Martin HL, Tomlinson DC, et al. (2013) A High-Throughput Assay to Identify Modifiers of Premature Chromosome Condensation. Journal of Biomolecular Screening.
18. Lin Z, Will Y (2012) Evaluation of Drugs With Specific Organ Toxicities in Organ-Specific Cell Lines. Toxicological Sciences 126: 114-127.
19. Kim JH, Chin BR, Kim SY, Kim JR, H.; BS (2003) Differential sensitivity of Taxol-induced apoptosis in U2OS and SaOS2 osteogenic sarcoma cells. Cancer Res Treat 35: 148-153.
20. Eom Y-W, Kim MA, Park SS, Goo MJ, Kwon HJ, et al. (2005) Two distinct modes of cell death induced by doxorubicin: apoptosis and cell death through mitotic catastrophe accompanied by senescence-like phenotype. Oncogene 24: 4765-4777. (Pubitemid 41076294)
21. Pang B, Qiao X, Janssen L, Velds A, Groothuis T, et al. (2013) Drug-induced histone eviction from open chromatin contributes to the chemotherapeutic effects of doxorubicin. Nat Commun 4: 1908.
22. Jordan MA, Toso RJ, Thrower D, Wilson L (1993) Mechanism of mitotic block and inhibition of cell proliferation by taxol at low concentrations. Proceedings of the National Academy of Sciences 90: 9552-9556. (Pubitemid 23315823)
23. Suzuki T, Fujikura K, Higashiyama T, Takata K (1997) DNA Staining for Fluorescence and Laser Confocal Microscopy. Journal of Histochemistry & Cytochemistry 45: 49-53. (Pubitemid 27045364)
24. Elmehdawi F, Wheway G, Szymanska K, Adams M, High AS, et al. (2013) Human Homolog of Drosophila Ariadne (HHARI) is a marker of cellular proliferation associated with nuclear bodies. Experimental Cell Research 319: 161-172.
25. Barabasz A, Foley B, Otto JC, Scott A, Rice J (2006) The use of high-content screening for the discovery and characterization of compounds that modulate mitotic index and cell cycle progression by differing mechanisms of action. Assay Drug Dev Technol 4: 153-163. (Pubitemid 43810919)
26. Martinez-Diez M, Santamaria G, Ortega AD, Cuezva JM (2006) Biogenesis and dynamics of mitochondria during the cell cycle: significance of 39UTRs. PLoS One 1: e107.
27. Birmingham A, Selfors LM, Forster T, Wrobel D, Kennedy CJ, et al. (2009) Statistical methods for analysis of high-throughput RNA interference screens. Nat Methods 6: 569-575.
28. Kaplowitz N (2005) Idiosyncratic drug hepatotoxicity. Nat Rev Drug Discov 4: 489-499.