In silico rational design and systems engineering of disulfide bridges in the catalytic domain of an alkaline α-amylase from Alkalimonas amylolytica to improve thermostability

Applied and Environmental Microbiology

Volumn 80, Issue 3, 2014, Pages 798-807

  Liu, Long ^a,b   Deng, Zhuangmei ^a,b   Yang, Haiquan ^a   Li, Jianghua ^a,b   Shin, Hyun Dong ^c   Chen, Rachel R ^c   Du, Guocheng ^a,b   Chen, Jian ^a,b  

^a JIANGNAN UNIVERSITY   (China)

^b Synergetic Innovation Center of Food Safety and Nutrition   (China)

^c Georgia Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOCHEMICAL PROPERTIES; CATALYTIC EFFICIENCIES; COMPARATIVE ANALYSIS; INDUSTRIAL ENZYMES; OPTIMUM TEMPERATURE; POSSIBLE MECHANISMS; TEXTILE PRODUCTION; WILD-TYPE ENZYMES;

AMINO ACIDS; AMYLASES; BRIDGES; COVALENT BONDS; MODEL STRUCTURES; STABILITY; SYSTEMS ENGINEERING;

ALKALINITY;

ALKALINITY; BACTERIUM; CATALYSIS; CHEMICAL BONDING; ENZYME ACTIVITY; MUTATION; REACTION KINETICS; TEXTILE INDUSTRY; THERMODYNAMIC PROPERTY;

AMYLASE; CYSTEINE; DISULFIDE;

ARTICLE; CHEMISTRY; ENZYME ACTIVE SITE; ENZYME STABILITY; ENZYMOLOGY; GAMMAPROTEOBACTERIA; GENETICS; METABOLISM; METHODOLOGY; PROTEIN ENGINEERING; TEMPERATURE;

ALPHA-AMYLASES; CATALYTIC DOMAIN; CYSTEINE; DISULFIDES; ENZYME STABILITY; GAMMAPROTEOBACTERIA; PROTEIN ENGINEERING; TEMPERATURE;

EID: 84892997262     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.03045-13     Document Type: Article

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