Comparative analysis of heterologous expression, biochemical characterization optimal production of an alkaline α-amylase from alkaliphilic Alkalimonas amylolytica in Escherichia coli and Pichia pastoris

Biotechnology Progress

Volumn 29, Issue 1, 2013, Pages 39-47

  Yang, Haiquan ^a   Liu, Long ^a   Shin, Hyun Dong ^b   Chen, Rachel R ^b   Li, Jianghua ^a   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Georgia Institute of Technology   (United States)

Author keywords

Alkalimonas amylolytica; Alkaline amylase; Escherichia coli; Pichia pastoris

Indexed keywords

ALKALIMONAS AMYLOLYTICA; ALKALIPHILIC; AMYLASE GENES; BIOCHEMICAL CHARACTERIZATION; CATALYTIC EFFICIENCIES; CODON USAGE; COMPARATIVE ANALYSIS; E. COLI; EXTRACELLULAR; HETEROLOGOUS EXPRESSION; LIQUID DETERGENTS; OPTIMAL CONDITIONS; OPTIMAL PRODUCTION; OPTIMUM TEMPERATURE; P. PASTORIS; PH STABILITY; PICHIA PASTORIS; SODIUM DODECYL SULFATE; SPECIFIC ACTIVITY; TWEEN 80; WASHING POWDER;

AMYLASES; COPPER; ESCHERICHIA COLI; MANGANESE; MANGANESE COMPOUNDS; METAL IONS; OPTIMIZATION; PH EFFECTS; SOAPS (DETERGENTS); SODIUM; SODIUM SULFATE; YEAST;

ALKALINITY;

AMYLASE;

ARTICLE; BIOSYNTHESIS; COMPARATIVE STUDY; ENZYMOLOGY; ESCHERICHIA COLI; GAMMAPROTEOBACTERIA; GENETICS; KINETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PH; PICHIA; TEMPERATURE;

ALPHA-AMYLASES; CLONING, MOLECULAR; ESCHERICHIA COLI; GAMMAPROTEOBACTERIA; HYDROGEN-ION CONCENTRATION; KINETICS; MOLECULAR SEQUENCE DATA; PICHIA; TEMPERATURE;

ALKALIMONAS AMYLOLYTICA; ESCHERICHIA COLI; PICHIA PASTORIS;

EID: 84873289324     PISSN: 87567938     EISSN: 15206033     Source Type: Journal    
DOI: 10.1002/btpr.1657     Document Type: Article

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