Systems engineering of tyrosine 195, tyrosine 260, and glutamine 265 in cyclodextrin glycosyltransferase from Paenibacillus macerans to enhance maltodextrin specificity for 2-O-d-glucopyranosyl-lascorbic acid synthesis

Applied and Environmental Microbiology

Volumn 79, Issue 2, 2013, Pages 672-677

  Han, Ruizhi ^a   Liu, Long ^a   Shin, Hyun Dong ^b   Chen, Rachel R ^b   Li, Jianghua ^a   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Georgia Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIZATION ACTIVITY; CYCLODEXTRIN GLYCOSYLTRANSFERASE; DISPROPORTIONATIONS; GLYCOSYL DONORS; HYDROGEN BONDING INTERACTIONS; KINETICS ANALYSIS; MALTODEXTRINS; PAENIBACILLUS MACERANS; PROTEIN ENGINEERING; SATURATION MUTAGENESIS; SIDE-CHAINS; STRUCTURE MODELING; SUBSTRATE SPECIFICITY; TRIPLE MUTANTS; WILD TYPES;

CYCLIZATION; GENETIC ENGINEERING; HYDROGEN BONDS; MUTAGENESIS; POLYSACCHARIDES;

AMINO ACIDS;

ASCORBIC ACID; ASCORBIC ACID 2 O GLUCOSIDE; ASCORBIC ACID 2-O-GLUCOSIDE; CYCLOMALTODEXTRIN GLUCANOTRANSFERASE; DRUG DERIVATIVE; GLUCOSYLTRANSFERASE; MALTODEXTRIN; MUTANT PROTEIN; POLYSACCHARIDE;

ASCORBIC ACID; BACTERIUM; BIOENGINEERING; ENZYME ACTIVITY; HYDROGEN; MUTAGENICITY; MUTATION; NUMERICAL MODEL; PROTEIN; SATURATION; SUGAR;

AMINO ACID SUBSTITUTION; ARTICLE; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; KINETICS; METABOLISM; PAENIBACILLUS; PROTEIN ENGINEERING; SITE DIRECTED MUTAGENESIS;

AMINO ACID SUBSTITUTION; ASCORBIC ACID; GLUCOSYLTRANSFERASES; KINETICS; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PAENIBACILLUS; POLYSACCHARIDES; PROTEIN ENGINEERING; SUBSTRATE SPECIFICITY;

PAENIBACILLUS MACERANS;

EID: 84871859885     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.02883-12     Document Type: Article

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