Structure-Guided Systems-Level Engineering of Oxidation-Prone Methionine Residues in Catalytic Domain of an Alkaline α-Amylase from Alkalimonas amylolytica for Significant Improvement of Both Oxidative Stability and Catalytic Efficiency

PLoS ONE

Volumn 8, Issue 3, 2013, Pages

  Yang, Haiquan ^a   Liu, Long ^a   Shin, Hyun Dong ^b   Li, Jianghua ^a   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

^b Georgia Institute of Technology   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALANINE; AMYLASE; ISOLEUCINE; METHIONINE; THREONINE;

ALKALIMONAS AMYLOLYTICA; ALKALINIZATION; AMINO ACID SUBSTITUTION; ARTICLE; CONTROLLED STUDY; ENZYME ACTIVE SITE; ENZYME ACTIVITY; ENZYME ENGINEERING; ENZYME STABILITY; HYDROGEN BOND; MUTATIONAL ANALYSIS; NONHUMAN; NUCLEOTIDE SEQUENCE; OXIDATION KINETICS; PH MEASUREMENT; POINT MUTATION; PROTEOBACTERIA; RESIDUE ANALYSIS; STRUCTURE ACTIVITY RELATION; STRUCTURE ANALYSIS; THERMOSTABILITY;

ALPHA-AMYLASES; AMINO ACID SUBSTITUTION; CATALYSIS; ENZYME STABILITY; GAMMAPROTEOBACTERIA; METHIONINE; MUTATION, MISSENSE; OXIDATION-REDUCTION; PROTEIN STRUCTURE, TERTIARY;

EID: 84875019963     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0057403     Document Type: Article

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