Purification and characterization of five alkaline, thermotolerant, and maltotetraose-producing α-amylases from Bacillus halodurans MS-2-5, and production of recombinant enzymes in Escherichia coli

Enzyme and Microbial Technology

Volumn 43, Issue 4-5, 2008, Pages 321-328

  Murakami, Shuichiro ^a   Nagasaki, Kenji ^a   Nishimoto, Haruka ^a   Shigematu, Ryo ^a   Umesaki, Jun ^a   Takenaka, Shinji ^a   Kaulpiboon, Jarunee ^b   Prousoontorn, Manchumas ^c   Limpaseni, Tipaporn ^c   Pongsawasdi, Piamsook ^c   Aoki, Kenji ^a  

^a KOBE UNIVERSITY   (Japan)

^b THAMMASAT UNIVERSITY   (Thailand)

^c CHULALONGKORN UNIVERSITY   (Thailand)

Author keywords

Alkaline amylase; Bacillus halodurans; Maltotetraose; Thermotolerance

Indexed keywords

ALCOHOLS; AMINES; AMINO ACIDS; BACTERIOLOGY; CATALYSTS; CULTIVATION; ESCHERICHIA COLI; FOOD ADDITIVES; GLUCOSE; ORGANIC ACIDS; PROTEINS; PURIFICATION; SULFATE MINERALS;

AMINO-ACID SEQUENCES; ANOMERIC; BACILLUS HALODURANS; CULTIVATION CONDITIONS; D SORBITOLS; D-GLUCOSE; E. COLI; ENZYME REACTIONS; ISOLATED STRAINS; LURIA-BERTANI MEDIUM; MALTOTETRAOSE; MOLECULAR MASSES; PROTEOLYTIC DEGRADATION; PURIFIED ENZYMES; REACTION PRODUCTS; RECOMBINANT ENZYMES; THERMOTOLERANCE;

ENZYMES;

AMYLASE; AMYLASE B; AMYLASE C; AMYLASE D; AMYLASE E; CARBOHYDRATE DERIVATIVE; GLUCOSE; MAGNESIUM SULFATE; MALTOTETROSE; RECOMBINANT ENZYME; SORBITOL;

ALKALINITY; ARTICLE; BACILLUS; BACILLUS HALODURANS; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM TRANSFORMATION; CHEMICAL REACTION; CULTURE MEDIUM; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME STABILITY; ENZYME SYNTHESIS; ESCHERICHIA COLI; HEAT TOLERANCE; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PROTEIN DEGRADATION; SEQUENCE ANALYSIS; THERMOSTABILITY;

BACILLUS HALODURANS; ESCHERICHIA COLI; LURIA;

EID: 49749105587     PISSN: 01410229     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.enzmictec.2008.05.006     Document Type: Article

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