A real-time, fluorescence-based assay for measuring μ-opioid receptor modulation of adenylyl cyclase activity in chinese hamster ovary cells

Journal of Biomolecular Screening

Volumn 19, Issue 2, 2014, Pages 223-231

  Knapman, Alisa ^a   Abogadie, Fe ^b,c   McIntrye, Peter ^b,c   Connor, Mark ^a  

^a MACQUARIE UNIVERSITY   (Australia)

^b UNIVERSITY OF MELBOURNE   (Australia)

^c RMIT UNIVERSITY   (Australia)

Author keywords

calcitonin; cAMP; high throughput; membrane potential; opioid

Indexed keywords

ADENYLATE CYCLASE; BUPRENORPHINE; CALCITONIN; CYCLIC AMP; ENKEPHALIN[2 DEXTRO ALANINE 4 METHYLPHENYLALANINE 5 GLYCINE]; FLUORESCENT DYE; FORSKOLIN; MORPHINE; MU OPIATE RECEPTOR; MU OPIATE RECEPTOR AGONIST; PERTUSSIS TOXIN;

ANIMAL CELL; ARTICLE; CELL MEMBRANE POTENTIAL; CHO CELL; CONCENTRATION RESPONSE; CONTROLLED STUDY; DESENSITIZATION; ENZYME ACTIVITY; ENZYME INHIBITION; FLUORESCENCE ANALYSIS; HUMAN; HYPERPOLARIZATION; NONHUMAN; PRIORITY JOURNAL; SIGNAL TRANSDUCTION;

CALCITONIN; CAMP; HIGH THROUGHPUT; MEMBRANE POTENTIAL; OPIOID;

ADENYLATE CYCLASE; ANIMALS; CHO CELLS; COLFORSIN; CRICETINAE; CRICETULUS; CYCLIC AMP; ENKEPHALIN, ALA(2)-MEPHE(4)-GLY(5)-; FLUORESCENCE; HUMANS; PERTUSSIS TOXIN; RECEPTORS, OPIOID, MU; SIGNAL TRANSDUCTION;

EID: 84892775419     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057113501391     Document Type: Article

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