Luminescent Biosensors for Real-Time Monitoring of Intracellular cAMP

Methods in Molecular Biology

Volumn 756, Issue , 2011, Pages 263-271

  Binkowski, Brock F ^a   Fan, Frank ^a   Wood, Keith V ^a  

^a PROMEGA CORPORATION   (United States)

Author keywords

Biosensor; cAMP; G protein coupled receptor; GloSensor; Inverse agonist; Live cell assay

Indexed keywords

CYCLIC AMP; G PROTEIN COUPLED RECEPTOR; HYBRID PROTEIN; LUCIFERASE; LUMINESCENT AGENT;

ANIMAL; ARTICLE; CELL LINE; ENZYMOLOGY; FIREFLY; GENETIC PROCEDURES; GENETIC TRANSFECTION; GENETICS; HUMAN; LUMINESCENCE; METABOLISM; METHODOLOGY; SIGNAL TRANSDUCTION;

ANIMALS; BIOSENSING TECHNIQUES; CELL LINE; CYCLIC AMP; FIREFLIES; HUMANS; LUCIFERASES; LUMINESCENT AGENTS; LUMINESCENT MEASUREMENTS; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION; TRANSFECTION;

EID: 80054723816     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-160-4_14     Document Type: Chapter

References (4)

1. Fan, F., Binkowski, B. F., Butler, B. L., Stecha, P. F., Lewis, M. K. and Wood, K. V. (2008) Novel genetically encoded biosensors using firefly luciferase. ACS Chem Biol 3, 346–51.
2. Binkowski, B. F., Fan, F. and Wood, K. V. (2009) Live-cell luminescent assays for GPCR studies. Gen Eng Biotech 29, 30–31.
3. Binkowski, B. F., Fan, F. and Wood, K.V. (2009) Engineered luciferases for molecular sensing in living cells. Curr Opin Biotech 20, 14–18.
4. Willoughby, D. and Cooper, D. M. F. (2008) Live-cell imaging of cAMP dynamics. Nat Methods 5, 29–36.