Asymmetric overlap extension PCR method bypassing intermediate purification and the amplification of wild-type template in site-directed mutagenesis

Biotechnology Letters

Volumn 29, Issue 6, 2007, Pages 925-930

  Xiao, Yue Hua ^a   Yin, Meng Hui ^a   Hou, Lei ^a   Luo, Ming ^a   Pei, Yan ^a  

^a SOUTHWEST UNIVERSITY   (China)

Author keywords

Asymmetric overlap extension PCR; Asymmetric PCR; Overlap extension PCR; Site directed mutagenesis

Indexed keywords

BINDING SITES; GENES; GENETIC ENGINEERING; MUTAGENESIS; PURIFICATION;

ASYMMETRIC OVERLAP EXTENSION (AOE); ASYMMETRIC OVERLAP EXTENSION PCR; OVERLAP EXTENSION PCR; SITE-DIRECTED MUTAGENESIS;

ENZYME KINETICS;

MONOMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; VEGETABLE PROTEIN;

ARTICLE; BIOLOGICAL MODEL; COTTON; GENETICS; METHODOLOGY; POINT MUTATION; POLYMERASE CHAIN REACTION; SITE DIRECTED MUTAGENESIS;

GOSSYPIUM; MODELS, GENETIC; MONOMERIC GTP-BINDING PROTEINS; MUTAGENESIS, SITE-DIRECTED; PLANT PROTEINS; POINT MUTATION; POLYMERASE CHAIN REACTION;

GOSSYPIUM HIRSUTUM;

EID: 34247354935     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-007-9327-4     Document Type: Article

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