Destabilization of the homotetrameric assembly of 3-deoxy-d-arabino- heptulosonate-7-phosphate synthase from the hyperthermophile pyrococcus furiosus enhances enzymatic activity

Journal of Molecular Biology

Volumn 426, Issue 3, 2014, Pages 656-673

  Nazmi, Ali Reza ^a   Schofield, Linley R ^b   Dobson, Renwick C J ^a,c   Jameson, Geoffrey B ^b   Parker, Emily J ^a  

^a UNIVERSITY OF CANTERBURY   (New Zealand)

^b MASSEY UNIVERSITY   (New Zealand)

^c UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

3 deoxy d arabino heptulosonate 7 phosphate synthase; analytical ultracentrifugation; mutagenesis; quaternary structure; shikimate pathway

Indexed keywords

3 DEOXY 7 PHOSPHOHEPTULONATE SYNTHASE; TETRAMER; ASPARAGINE; ISOLEUCINE; MONOMER;

ALLOSTERISM; ANISOTROPY; ARTICLE; BACTERIAL STRAIN; CONTROLLED STUDY; CRYSTAL STRUCTURE; ENZYME ACTIVITY; ENZYME KINETICS; ENZYME STABILITY; GEL PERMEATION CHROMATOGRAPHY; HYDROGEN BOND; NATIVE POLYACRYLAMIDE GEL ELECTROPHORESIS; NONHUMAN; PH; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN EXPRESSION; PROTEIN QUATERNARY STRUCTURE; PYROCOCCUS FURIOSUS; SEDIMENTATION RATE; SITE DIRECTED MUTAGENESIS; SURFACE PROPERTY; THERMOSTABILITY; ULTRACENTRIFUGATION; WILD TYPE; X RAY CRYSTALLOGRAPHY; AMINO ACID SUBSTITUTION; CATALYST; DIMERIZATION; DISSOCIATION CONSTANT; ENZYME ANALYSIS; ENZYME KINETIC ASSAY; ENZYME MECHANISM; GENE DISRUPTION; HYDROPHOBICITY; HYPERTHERMOPHILIC ARCHAEON; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN HOMEOSTASIS; PROTEIN PROTEIN INTERACTION;

ARCHAEA; PYROCOCCUS FURIOSUS;

3-DEOXY-D-ARABINO-HEPTULOSONATE-7-PHOSPHATE SYNTHASE; ANALYTICAL ULTRACENTRIFUGATION; DIFFERENTIAL SCANNING CALORIMETRY; DIFFERENTIAL SCANNING FLUORIMETRY; DSC; DSF; MUTAGENESIS; PEG; POLYETHYLENE GLYCOL; QUATERNARY STRUCTURE; SAXS; SHIKIMATE PATHWAY; SMALL-ANGLE X-RAY SCATTERING;

3-DEOXY-7-PHOSPHOHEPTULONATE SYNTHASE; BINDING SITES; CHROMATOGRAPHY, GEL; CRYSTALLOGRAPHY, X-RAY; ENZYME STABILITY; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; PYROCOCCUS FURIOSUS; SCATTERING, SMALL ANGLE;

EID: 84892522972     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2013.11.008     Document Type: Article

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