Expression, purification, and characterization of 3-deoxy-D-arabino- heptulosonate 7-phosphate synthase from Pyrococcus furiosus

Protein Expression and Purification

Volumn 34, Issue 1, 2004, Pages 17-27

  Schofield, Linley R ^a   Patchett, Mark L ^a   Parker, Emily J ^a  

^a MASSEY UNIVERSITY   (New Zealand)

Author keywords

3 Deoxy D arabino heptulosonate 7 phosphate synthase; Metalloenzyme; Pyrococcus furiosus; Shikimate pathway

Indexed keywords

ESCHERICHIA COLI; PYROCOCCUS; PYROCOCCUS FURIOSUS;

EID: 1442264878     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2003.11.008     Document Type: Article

References (44)

1. R. Bentley, The shikimate pathway - a metabolic tree with many branches, Crit. Rev. Biochem. Mol. Biol. 25 (1990) 307-384.
2. K.M. Herrmann, L.M. Weaver, The shikimate pathway, Annu. Rev. Plant Physiol. Plant Mol. Biol. 50 (1999) 473-503.
3. G.E. Walker, B. Dunbar, I.S. Hunter, H.G. Nimmo, J.R. Coggins, Evidence for a novel class of microbial 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase in Streptomyces coelicolor A3(2), Streptomyces rimosus and Neurospora crassa, Microbiology 142 (1996) 1973-1982.
4. G. Gosset, C.A. Bonner, R.A. Jensen, Microbial origin of plant-type 2-keto-3-deoxy-D-arabino-heptulosonate 7-phosphate synthases, exemplified by the chorismate-and tryptophan-regulated enzyme from Xanthomonas campestris, J. Bacteriol. 183 (2001) 4061-4070.
5. P.S. Subramaniam, G. Xie, T. Xia, R.A. Jensen, Substrate ambiguity of 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Neisseria gonorrhoeae in the context of its membership in a protein family containing a subset of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthases, J. Bacteriol. 180 (1998) 119-127.
6. R.A. Jensen, G. Xie, D.H. Calhoun, C.A. Bonner, The correct phylogenetic relationship of KdsA (3-deoxy-D-manno-octulosonate 8-phosphate synthase) with one of two independently evolved classes of AroA (3-deoxy-D-arabino- heptulosonate 7-phosphate synthase), J. Mol. Evol. 54 (2002) 416-423.
7. M.R. Birck, R.W. Woodard, Aquifex aeolicus 3-deoxy-D-manno-2-octulosonic acid 8-phosphate synthase: A new class of KDO 8-P synthase?, J. Mol. Evol. 52 (2001) 205-214.
8. R.A. Jensen, E.W. Nester, Regulatory enzymes of aromatic amino acid biosynthesis in Bacillus subtilis. I. Purification and properties of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase, J. Biol. Chem. 241 (1966) 3365-3372.
9. H.S. Duewel, G.Y. Sheflyan, R.W. Woodard, Functional and biochemical characterization of a recombinant 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase from the hyperthermophilic bacterium Aquifex aeolicus, Biochem. Biophys. Res. Commun. 263 (1999) 346-351.
10. H.S. Duewel, R.W. Woodard, A metal bridge between two enzyme families. 3-Deoxy-D-manno-octulosonate 8-phosphate synthase from Aquifex aeolicus requires a divalent metal for activity, J. Biol. Chem. 275 (2000) 22824-22831.
11. P.H. Ray, Purification and characterization of 3-deoxy-D-D-manno- octulosonate 8-phosphate synthetase from Escherichia coli, J. Bacteriol. 141 (1980) 635-644.
12. I.A. Shumilin, R.H. Kretsinger, R.H. Bauerle, Crystal structure of phenylalanine-regulated 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Escherichia coli, Structure 7 (1999) 865-875.
13. M. Hartmann, T.R. Schneider, A. Pfeil, G. Heinrich, W.N. Lipscomb, G.H. Braus, Evolution of feedback-inhibited β/α barrel isoenzymes by gene duplication and a single mutation, Proc. Natl. Acad. Sci. USA 100 (2003) 862-867.
14. H.S. Duewel, S. Radaev, J. Wang, R.W. Woodard, D.L. Gatti, Substrate and metal complexes of 3-deoxy-D-manno-octulosonate 8-phosphate synthase from Aquifex aeolicus at 1.9-Å resolution. Implications for the condensation mechanism, J. Biol. Chem. 276 (2001) 8393-8402.
15. S. Radaev, P. Dastidar, M. Patel, R.W. Woodard, D.L. Gatti, Structure and mechanism of 3-deoxy-D-manno-octulosonate 8-phosphate synthase, J. Biol. Chem. 275 (2000) 9476-9484.
16. G. Fiala, K.O. Stetter, Pyrococcus furiosus sp. nov. represents a novel genus of marine heterotrophic archaebacteria growing optimally at 100°C, Arch. Microbiol. 145 (1986) 56-61.
17. M.L. Patchett, T.L. Neal, L.R. Schofield, R.C. Strange, R.M. Daniel, H.W. Morgan, Heat treatment purification of thermostable cellulase and hemicellulase enzymes expressed in E. coli, Enzyme Microb. Technol. 11 (1989) 113-115.
18. M.C. Bewley, J.S. Lott, E.N. Baker, M.L. Patchett, The cloning, expression and crystallization of a thermostable arginase, FEBS Lett. 386 (1996) 215-218.
19. A.A. McCarthy, H.M. Baker, S.C. Shewry, T.F. Kagawa, E. Saafi, M.L. Patchett, E.N. Baker, Expression, crystallization and preliminary characterization of methylmalonyl coenzyme A epimerase from Propionibacterium shermanii, Acta Crystallogr. D 57 (2001) 706-708.
20. R. Schoner, K.M. Herrmann, 3-Deoxy-D-arabino-heptulosonate 7-phosphate synthase. Purification, properties, and kinetics of the tyrosine-sensitive isoenzyme from Escherichia coli, J. Biol. Chem. 251 (1976) 5440-5447.
21. P.H. Ray, 3-Deoxy-D-manno-octulosonate 8-phosphate (KDO-8-P) synthase, Methods Enzymol. 83 (1982) 525-530.
22. P.A. Lanzetta, L.J. Alvarez, P.S. Reinach, O.A. Candia, An improved assay for nanomole amounts of inorganic phosphate, Anal. Biochem. 100 (1979) 95-97.
23. S. Tabor, C.C. Richardson, A bacteriophage T7 RNA polymerase/promoter system for controlled exclusive expression of specific genes, Proc. Natl. Acad. Sci. USA 82 (1985) 1074-1078.
24. C.C. Duke, J.K. MacLeod, J.F. Williams, Nuclear magnetic resonance studies of D-erythrose 4-phosphate in aqueous solution. Structures of the major contributing monomeric and dimeric forms, Carbohydr. Res. 95 (1981) 1-26.
25. E.J. Parker, E.M.M. Bulloch, G.B. Jameson, C. Abell, Substrate deactivation of phenylalanine-sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase by erythrose 4-phosphate, Biochemistry 40 (2001) 14821-14828.
26. U.K. Laemmli, Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature (London) 227 (1970) 680-685.
27. A. Shevchenko, M. Wilm, O. Vorm, M. Mann, Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels, Anal. Chem. 68 (1996) 850-858.
28. M.M. Bradford, A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding, Anal. Biochem. 72 (1976) 248-254.
29. D.E. Gueffroy (Ed.), A Guide for the Preparation and Use of Buffers in Biological Systems, Calbiochem, La Jolla, CA, 1975.
30. R.M. Daniel, M.J. Danson, Assaying activity and assessing thermostability of hyperthermophilic enzymes, Methods Enzymol. 334 (2001) 283-293.
31. F. Wold, C.E. Ballou, Studies on the enzyme enolase. I. Equilibrium studies, J. Biol. Chem. 227 (1957) 301-312.
32. P.F. Blackmore, J.F. Williams, J.K. MacLeod, Dimerization of erythrose 4-phosphate, FEBS Lett. 64 (1976) 222-226.
33. J.F. Williams, P.F. Blackmore, C.C. Duke, J.K. MacLeod, Fact, uncertainty and speculation concerning the biochemistry of D-erythrose 4-phosphate and its metabolic roles, Int. J. Biochem. 12 (1980) 339-344.
34. A.M. Grunden, M. Ghosh, M.W.W. Adams, Proline dipeptidase from Pyrococcus furiosus, Methods Enzymol. 330 (2001) 433-445.
35. L.S. Chang, P.M. Hicks, R.M. Kelly, Protease I from Pyrococcus furiosus, Methods Enzymol. 330 (2001) 403-413.
36. F. Gentile, P. Amodeo, F. Febbraio, F. Picaro, A. Motta, S. Formisano, R. Nucci, SDS-resistant active and thermostable dimers are obtained from the dissociation of homotetrameric β-glycosidase from hyperthermophilic Sulfolobus solfataricus in SDS. Stabilizing role of the A-C intermonomeric interface, J. Biol. Chem. 277 (2002) 44050-44060.
37. G.D. Dotson, R.K. Dua, J.C. Clemens, E.W. Wooten, R.W. Woodard, Overproduction and one-step purification of Escherichia coli 3-deoxy-D-manno-octulosonic acid 8-phosphate synthase and oxygen transfer studies during catalysis using isotopic-shifted heteronuclear NMR, J. Biol. Chem. 270 (1995) 13698-13705.
38. R.J. McCandliss, M.D. Poling, K.M. Hermann, 3-Deoxy-D-arabino- heptulosonate 7-phosphate synthase. Purification and molecular characterization of the phenylalanine-sensitive isoenzyme from Escherichia coli, J. Biol. Chem. 253 (1978) 4259-4265.
39. J.M. Ray, R. Bauerle, Purification and properties of tryptophan- sensitive 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Escherichia coli, J. Bacteriol. 173 (1991) 1894-1901.
40. W.P. Taylor, G.Y. Sheflyan, R.W. Woodard, A single point mutation in 3-deoxy-D-manno-octulosonate 8-phosphate synthase is responsible for temperature sensitivity in a mutant strain of Salmonella typhimurium, J. Biol. Chem. 275 (2000) 32141-32146.
41. M.L. Patchett, R.M. Daniel, H.W. Morgan, Characterization of arginase from the extreme thermophile 'Bacillus caldovelox', Biochim. Biophys. Acta 1077 (1991) 291-298.
42. R.M. Daniel, M. Dines, H.H. Petach, The denaturation and degradation of stable enzymes at high temperatures, Biochem. J. 317 (1996) 1-11.
43. L. Huang, W.M. Nakatsukasa, E. Nester, Regulation of aromatic amino acid biosynthesis in Bacillus subtilis 168. Purification, characterization, and subunit structure of the bifunctional enzyme 3-deoxy-D-arabino-heptulosonate 7-phosphate synthetase-chorismate mutase, J. Biol. Chem. 249 (1974) 4467-4472.
44. C.M. Stephens, R. Bauerle, Analysis of the metal requirement of 3-deoxy-D-arabino-heptulosonate 7-phosphate synthase from Escherichia coli, J. Biol. Chem. 266 (1991) 20810-20817.