Advances in silkworm studies accelerated by the genome sequencing of bombyx mori

Annual Review of Entomology

Volumn 59, Issue , 2014, Pages 513-536

  Xia, Qingyou ^a   Li, Sheng ^b   Feng, Qili ^c  

^a SOUTHWEST UNIVERSITY   (China)

^b INSTITUTE OF PLANT PHYSIOLOGY AND ECOLOGY   (China)

^c SOUTH CHINA NORMAL UNIVERSITY   (China)

Author keywords

Genetic manipulation; Genomics; Innate immunity; Metamorphosis; Silk gland; Silk protein

Indexed keywords

INSECT PROTEIN; PROTEOME; SILK;

ANIMAL; BOMBYX; GENETICS; GROWTH, DEVELOPMENT AND AGING; IMMUNOLOGY; INSECT GENOME; LARVA; METABOLISM; PHYSIOLOGY;

ANIMALS; BOMBYX; GENOME, INSECT; INSECT PROTEINS; LARVA; PROTEOME; SILK;

EID: 84891911443     PISSN: 00664170     EISSN: None     Source Type: Book Series    
DOI: 10.1146/annurev-ento-011613-161940     Document Type: Article

References (148)

1. Ando T, Fujiwara H. 2013. Electroporation-mediated somatic transgenesis for rapid functional analysis in insects. Development 140:454-58
2. Arunkumar KP, Metta M, Nagaraju J. 2006. Molecular phylogeny of silkmoths reveals the origin of domesticated silkmoth, Bombyx mori from Chinese Bombyx mandarina and paternal inheritance of Antheraea proylei mitochondrial DNA. Mol. Phylogenet. Evol. 40:419-27
3. Atsumi S, Miyamoto K, Yamamoto K, Narukawa J, Kawai S, et al. 2012. Single amino acid mutation in an ATP-binding cassette transporter gene causes resistance to Bt toxin Cry1Ab in the silkworm, Bombyx mori. Proc. Natl. Acad. Sci. USA 109:E1591-98
4. Beldade P, Saenko SV, PulN, Long AD. 2009. A gene-based linkage map for Bicyclus anynana butterflies allows for a comprehensive analysis of synteny with the lepidopteran reference genome. PLoS Genet. 5:e1000366
5. Cai Y, Zhou Q, Yu C, Wang X, Hu S, et al. 2012. Transposable-element associated small RNAs in Bombyx mori genome. PLoS One 7:e36599
6. Casati B, Terova G, Cattaneo AG, Rimoldi S, Franzetti E, et al. 2012. Molecular cloning, characterization and expression analysis of ATG1 in the silkworm, Bombyx mori. Gene 511:326-37
7. Chen X, Cai H, Ling S, Shao Z, Huang Y. 2012. Conformation transition of Bombyx mori silk protein monitored by time-dependent Fourier transform infrared (FT-IR) spectroscopy: Effect of organic solvent. Appl. Spectrosc. 66:696-99
8. Chen X, Shao Z, Knight DP, Vollrath F. 2007. Conformation transition kinetics of Bombyx mori silk protein. Proteins 68:223-31
9. Cheng D, Xia Q, Duan J, Wei L, Huang C, et al. 2008. Nuclear receptors in Bombyx mori: Insights into genomic structure and developmental expression. Insect Biochem. Mol. Biol. 38:1130-37
10. Cheng T, Zhao P, Liu C, Xu P, Gao Z, et al. 2006. Structures, regulatory regions, and inductive expression patterns of antimicrobial peptide genes in the silkworm Bombyx mori. Genomics 87:356-65
11. Cheng TC, Zhang YL, Liu C, Xu PZ, Gao ZH, et al. 2008. Identification and analysis of Toll-related genes in the domesticated silkworm, Bombyx mori. Dev. Comp. Immunol. 32:464-75
12. Dai H, Ma L, Wang J, Jiang R, Wang Z, Fei J. 2008. Knockdown of ecdysis-triggering hormone gene with a binary UAS/GAL4 RNA interference system leads to lethal ecdysis deficiency in silkworm. Acta Biochim. Biophys. Sin. (Shanghai) 40:790-95
13. Daimon T, Kozaki T, Niwa R, Kobayashi I, Furuta K, et al. 2012. Precocious metamorphosis in the juvenile hormone-deficient mutant of the silkworm, Bombyx mori. PLoS Genet. 8:e1002486
14. Dash BC, Mandal BB, Kundu SC. 2009. Silk gland sericin protein membranes: Fabrication and characterization for potential biotechnological applications. J. Biotechnol. 144:321-29
15. Deng H, Zhang J, Li Y, Zheng S, Liu L, et al. 2012. Homeodomain POU and Abd-A proteins regulate the transcription of pupal genes during metamorphosis of the silkworm, Bombyx mori. Proc. Natl. Acad. Sci. USA 109:12598-603
16. Deng H, Zheng S, Yang X, Liu L, Feng Q. 2011. Transcription factors BmPOUM2and BmbetaFTZ-F1 are involved in regulation of the expression of the wing cuticle protein gene BmWCP4 in the silkworm, Bombyx mori. Insect Mol. Biol. 20:45-60
17. Duan J, Li R, Cheng D, Fan W, Zha X, et al. 2010. SilkDB v2.0: A platform for silkworm (Bombyx mori) genome biology. Nucleic Acids Res. 38:D453-56
18. Duan J, Xu H, Ma S, Guo H,Wang F, et al. 2013. Cre-mediated targeted gene activation in the middle silk glands of transgenic silkworms (Bombyx mori) . Transgenic Res. 22:607-19
19. Furukawa S, Tanaka H, Ishibashi J, Imanishi S, Yamakawa M. 2009. Functional characterization of a cactus homolog from the silkworm Bombyx mori. Biosci. Biotechnol. Biochem. 73:2665-70
20. Futahashi R, Sato J, Meng Y, Okamoto S, Daimon T, et al. 2008. yellow and ebony are the responsible genes for the larval color mutants of the silkworm Bombyx mori. Genetics 180:1995-2005
21. Gan L, Liu X, Xiang Z, He N. 2011. Microarray-based gene expression profiles of silkworm brains. BMC Neurosci. 12:8
22. Goldsmith MR, Shimada T, Abe H. 2005. The genetics and genomics of the silkworm, Bombyx mori. Annu. Rev. Entomol. 50:71-100
23. Goncu E, Parlak O. 2008. Some autophagic and apoptotic features of programmed cell death in the anterior silk glands of the silkworm, Bombyx mori. Autophagy 4:1069-72
24. Goncu E, Parlak O. 2009. Morphological changes and patterns of ecdysone receptor B1 immunolocalization in the anterior silk gland undergoing programmed cell death in the silkworm, Bombyx mori. Acta Histochem. 111:25-34
25. Greving I, Cai M, Vollrath F, Schniepp HC. 2012. Shear-induced self-assembly of native silk proteins into fibrils studied by atomic force microscopy. Biomacromolecules 13:676-82
26. Gu SH, Lin JL, Lin PL. 2010. PTTH-stimulated ERK phosphorylation in prothoracic glands of the silkworm, Bombyx mori: Role of Ca2+/calmodulin and receptor tyrosine kinase. J. Insect Physiol. 56:93-101
27. Gu SH, Lin JL, Lin PL, Chen CH. 2009. Insulin stimulates ecdysteroidogenesis by prothoracic glands in the silkworm, Bombyx mori. Insect Biochem. Mol. Biol. 39:171-79
28. Gu SH, Yeh WL, Young SC, Lin PL, Li S. 2012. TOR signaling is involved in PTTH-stimulated ecdysteroidogenesis by prothoracic glands in the silkworm, Bombyx mori. Insect Biochem. Mol. Biol. 42:296- 303
29. Gu SH, Young SC, Lin JL, Lin PL. 2011. Involvement of PI3K/Akt signaling in PTTH-stimulated ecdysteroidogenesis by prothoracic glands of the silkworm, Bombyx mori. Insect Biochem. Mol. Biol. 41:197- 202
30. Gui ZZ, Lee KS, Kim BY, Choi YS,Wei YD, et al. 2006. Functional role of aspartic proteinase cathepsin D in insect metamorphosis. BMC Dev. Biol. 6:49
31. Guo E, He Q, Liu S, Tian L, Sheng Z, et al. 2012. MET is required for the maximal action of 20- hydroxyecdysone during Bombyx metamorphosis. PLoS One 7:e53256
32. Han MJ, Shen YH, Gao YH, Chen LY, Xiang ZH, Zhang Z. 2010. Burst expansion, distribution and diversification of MITEs in the silkworm genome. BMC Genomics 11:520
33. He YX, Zhang NN, Li WF, Jia N, Chen BY, et al. 2012. N-terminal domain of Bombyx mori fibroin mediates the assembly of silk in response to pH decrease. J. Mol. Biol. 418:197-207
34. Hou Y, Xia Q, Zhao P, Zou Y, LiuH, et al. 2007. Studies on middle and posterior silk glands of silkworm (Bombyx mori) using two-dimensional electrophoresis and mass spectrometry. Insect Biochem. Mol. Biol. 37:486-96
35. Hou Y, Zou Y, Wang F, Gong J, Zhong X, et al. 2010. Comparative analysis of proteome maps of silkworm hemolymph during different developmental stages. Proteome Sci. 8:45
36. Huang L, Cheng T, Xu P, Cheng D, Fang T, Xia Q. 2009. A genome-wide survey for host response of silkworm, Bombyx mori during pathogen Bacillus bombyseptieus infection. PLoS One 4:e8098
37. Huang Y, Zou Q, Shen XJ, Tang SM,Wang SP, Zhao QL. 2011. Differential expression of microRNA- 2b with potential target coding P25 in the fifth instar larvae posterior silk gland of the silkworm. Mol. Biol. (Mosk) 45:627-32
38. Iizuka M, Ogawa S, Takeuchi A, Nakakita S, Kubo Y, et al. 2009. Production of a recombinant mouse monoclonal antibody in transgenic silkworm cocoons. FEBS J. 276:5806-20
39. International Silkworm Genome Consortium. 2008. The genome of a lepidopteran model insect, the silkworm Bombyx mori. Insect Biochem. Mol. Biol. 38:1036-45
40. Ito K, Kidokoro K, Sezutsu H, Nohata J, Yamamoto K, et al. 2008. Deletion of a gene encoding an amino acid transporter in the midgut membrane causes resistance to a Bombyx parvo-like virus. Proc. Natl. Acad. Sci. USA 105:7523-27
41. Jagadeeswaran G, Zheng Y, Sumathipala N, Jiang H, Arrese EL, et al. 2010. Deep sequencing of small RNA libraries reveals dynamic regulation of conserved and novel microRNAs and microRNA-stars during silkworm development. BMC Genomics 11:52
42. Jia SH, Li MW, Zhou B, Liu WB, Zhang Y, et al. 2007. Proteomic analysis of silk gland programmed cell death during metamorphosis of the silkworm Bombyx mori. J. Proteome Res. 6:3003-10
43. Jiang L, Cheng T, Zhao P, Yang Q, Wang G, et al. 2012. Resistance to BmNPV via overexpression of an exogenous gene controlled by an inducible promoter and enhancer in transgenic silkworm, Bombyx mori. PLoS One 7:e41838
44. Jiang L, Zhao P, Cheng T, Sun Q, Peng Z, et al. 2013. A transgenic animal with antiviral properties that might inhibit multiple stages of infection. Antiviral Res. 98:171-73
45. Jiang L, Zhao P, Wang G, Cheng T, Yang Q, et al. 2013. Comparison of factors that may affect the inhibitory efficacy of transgenic RNAi targeting of baculoviral genes in silkworm, Bombyx mori. Antiviral Res. 97:255-63
46. Jindra M, Palli SR, Riddiford LM. 2012. The juvenile hormone signaling pathway in insect development. Annu. Rev. Entomol. 58:181-204
47. Kajiwara H, Imamaki A, Nakamura M, Mita K, Xia Q, IshizakaM. 2009. Proteome analysis of silkworm 1. Fat body. J. Electrophor. 53:19-26
48. Kajiwara H, Imamaki A, Nakamura M, Mita K, Xia Q, IshizakaM. 2009. Proteome analysis of silkworm 2. Hemolymph. J. Electrophor. 53:27-31
49. Kaneko Y, Kinjoh T, KiuchiM, Hiruma K. 2011. Stage-specific regulation of juvenile hormone biosynthesis by ecdysteroid in Bombyx mori. Mol. Cell. Endocrinol. 335:204-10
50. Kaneko Y, Yasanga T, Suzuki M, Sakurai S. 2011. Larval fat body cells die during the early pupal stage in the frame of metamorphosis remodelation in Bombyx mori. J. Insect Physiol. 57:1715-22
51. Kanginakudru S, Royer C, Edupalli SV, Jalabert A, Mauchamp B, et al. 2007. Targeting ie-1 gene by RNAi induces baculoviral resistance in lepidopteran cell lines and in transgenic silkworms. Insect Mol. Biol. 16:635-44
52. Kawaoka S, Katsuma S, Daimon T, Isono R, Omuro N, et al. 2008. Functional analysis of four Gloverinlike genes in the silkworm, Bombyx mori. Arch. Insect Biochem. Physiol. 67:87-96
53. Kayukawa T, Minakuchi C,Namiki T, Togawa T, YoshiyamaM, et al. 2012. Transcriptional regulation of juvenile hormone-mediated induction of Krüppel homolog 1, a repressor of insect metamorphosis. Proc. Natl. Acad. Sci. USA 109:11729-34
54. Kidokoro K, Ito K, Ogoyi DO, Abe H, Mita K, Kadono-Okuda K. 2010. Non-susceptibility genes to Bombyx densovirus type 1, Nid-1 and nsd-1, affect distinct steps of the viral infection pathway. J. Invertebr. Pathol. 103:79-81
55. Kimoto M, Kitagawa T, Kobayashi I, Nakata T, Kuroiwa A, Takiya S. 2012. Inhibition of the binding of MSG-intermolt-specific complex, MIC, to the sericin-1 gene promoter and sericin-1 gene expression by POU-M1/SGF-3. Dev. Genes Evol. 222:351-59
56. Kinjoh T, Kaneko Y, Itoyama K, Mita K, Hiruma K, Shinoda T. 2007. Control of juvenile hormone biosynthesis in Bombyx mori: Cloning of the enzymes in the mevalonate pathway and assessment of their developmental expression in the corpora allata. Insect Biochem. Mol. Biol. 37:808-18
57. Komoto N, Quan GX, Sezutsu H, Tamura T. 2009. A single-base deletion in an ABC transporter gene causes white eyes, white eggs, and translucent larval skin in the silkworm w-3oe mutant. Insect Biochem. Mol. Biol. 39:152-56
58. Li A, Zhao Q, Tang S, ZhangZ,Pan S, ShenG. 2005. Molecular phylogeny of the domesticated silkworm, Bombyx mori, based on the sequences of mitochondrial cytochrome b genes. J. Genet. 84:137-42
59. Li D, Guo Y, Shao H, Tellier LC, Wang J, et al. 2010. Genetic diversity, molecular phylogeny and selection evidence of the silkworm mitochondria implicated by complete resequencing of 41 genomes. BMC Evol. Biol. 10:81
60. Li G, Zhou P, Shao Z, Xie X, Chen X, et al. 2001. The natural silk spinning process. A nucleationdependent aggregation mechanism? Eur. J. Biochem. 268:6600-6
61. Li JY, Yang HJ, Lan TY, Wei H, Zhang HR, et al. 2011. Expression profiling and regulation of genes related to silkworm posterior silk gland development and fibroin synthesis. J. Proteome Res. 10:3551-64
62. Li M, Guo Q, Hou C, Miao X, Xu A, et al. 2006. Linkage and mapping analyses of the densonucleosis non-susceptible gene nsd-Z in the silkworm Bombyx mori using SSR markers. Genome 49:397-402
63. Li QR, Deng XJ, Huang ZJ, Zheng SC, Tettamanti G, et al. 2011. Expression of autophagy-related genes in the anterior silk gland of the silkworm (Bombyx mori) during metamorphosis. Can. J. Zool. 89:1019-26
64. Li QR, Deng XJ, Yang WY, Huang ZJ, Tettamanti G, et al. 2010. Autophagy, apoptosis, and ecdysisrelated gene expression in the silk gland of the silkworm (Bombyx mori) during metamorphosis. Can. J. Zool. 88:1169-78
65. Li S, Zhang QR, Xu WH, Schooley DA. 2005. Juvenile hormone diol kinase, a calcium-binding protein with kinase activity, from the silkworm, Bombyx mori. Insect Biochem. Mol. Biol. 35:1235-48
66. Li XH,Wu XF, YueWF, Liu JM, Li GL, Miao YG. 2006. Proteomic analysis of the silkworm (Bombyx mori L.) hemolymph during developmental stage. J. Proteome Res. 5:2809-14
67. Li Y, Wang G, Tian J, Liu H, Yang H, et al. 2012. Transcriptome analysis of the silkworm (Bombyx mori) by high-throughput RNA sequencing. PLoS One 7:e43713
68. Li Y, Zhao P, Liu S, Dong Z, Chen J, et al. 2012. A novel protease inhibitor in Bombyx mori is involved in defense against Beauveria bassiana. Insect Biochem. Mol. Biol. 42:766-75
69. Li Z-Q, Cheng D-J,Wei L, Zhao P, Shu X, et al. 2010. The silkworm homolog of Methoprene-tolerant (Met) gene reveals sequence conservation but function divergence. Insect Sci. 17:313-24
70. Lin JL, Gu SH. 2007. In vitro and in vivo stimulation of extracellular signal-regulated kinase (ERK) by the prothoracicotropic hormone in prothoracic gland cells and its developmental regulation in the silkworm, Bombyx mori. J. Insect Physiol. 53:622-31
71. Liu C, Yamamoto K, Cheng TC, Kadono-Okuda K, Narukawa J, et al. 2010. Repression of tyrosine hydroxylase is responsible for the sex-linked chocolatemutation of the silkworm, Bombyx mori. Proc. Natl. Acad. Sci. USA 107:12980-85
72. Liu S, Li D, Li Q, Zhao P, Xiang Z, Xia Q. 2010. MicroRNAs of Bombyx mori identified by Solexa sequencing. BMC Genomics 11:148
73. Liu Y, Zhou S, Ma L, Tian L, Wang S, et al. 2010. Transcriptional regulation of the insulin signaling pathway genes by starvation and 20-hydroxyecdysone in the Bombyx fat body. J. Insect Physiol. 56:1436-44
74. Long DP, Zhao AC, Chen XJ, Zhang Y, Lu WJ, et al. 2012. FLP recombinase-mediated site-specific recombination in silkworm, Bombyx mori. PLoS One 7:e40150
75. Ma L, Liu S, Shi M, Chen XX, Li S. 2013. Ras1CA-upregulated bcpi inhibits cathepsin activity to prevent tissue destruction of the Bombyx posterior silk gland. J. Proteome Res. 12:1924-34
76. Ma L, Xu H, Zhu J,Ma S, Liu Y, et al. 2011. Ras1CA overexpression in the posterior silk gland improves silk yield. Cell Res. 21:934-43
77. Ma S, Zhang S, Wang F, Liu Y, Xu H, et al. 2012. Highly efficient and specific genome editing in silkworm using custom TALENs. PLoS One 7:e45035
78. Meng Y, Katsuma S, Daimon T, Banno Y, Uchino K, et al. 2009. The silkworm mutant lemon (lemon lethal) is a potential insect model for human sepiapterin reductase deficiency. J. Biol. Chem. 284:11698- 705
79. Mita K, Kasahara M, Sasaki S, Nagayasu Y, Yamada T, et al. 2004. The genome sequence of silkworm, Bombyx mori. DNA Res. 11:27-35
80. Nita M, Wang HB, Zhong YS, Mita K, Iwanaga M, Kawasaki H. 2009. Analysis of ecdysone-pulse responsive region of BMWCP2 in wing disc of Bombyx mori. Comp. Biochem. Physiol. B 153:101-8
81. Okamoto N, Yamanaka N, Satake H, Saegusa H, Kataoka H, Mizoguchi A. 2009. An ecdysteroidinducible insulin-like growth factor-like peptide regulates adult development of the silkmoth Bombyx mori. FEBS J. 276:1221-32
82. Osanai-Futahashi M, Ohde T, Hirata J, Uchino K, Futahashi R, et al. 2012. A visible dominant marker for insect transgenesis. Nat. Commun. 3:1295
83. Osanai-FutahashiM, Suetsugu Y, Mita K, Fujiwara H. 2008. Genome-wide screening and characterization of transposable elements and their distribution analysis in the silkworm, Bombyx mori. Insect Biochem. Mol. Biol. 38:1046-57
84. PanG, Xu J, Li T, Xia Q, Liu SL, et al. 2013. Comparative genomics of parasitic silkworm microsporidia reveal an association between genome expansion and host adaptation. BMC Genomics 14:186
85. Pringle EG, Baxter SW,Webster CL, Papanicolaou A, Lee SF, Jiggins CD. 2007. Synteny and chromosome evolution in the Lepidoptera: Evidence from mapping in Heliconius melpomene. Genetics 177:417-26
86. Sajwan S, Takasu Y, Tamura T, Uchino K, Sezutsu H, Zurovec M. 2013. Efficient disruption of endogenous Bombyx gene by TAL effector nucleases. Insect Biochem. Mol. Biol. 43:17-23
87. Sakamoto M, Ohta M, Suzuki A, Takase H, Yoshizawa Y, et al. 2011. Localization of the serine protease homolog BmSPH-1 in nodules of E. Coli-injected Bombyx mori larvae and functional analysis of its role in nodule melanization. Dev. Comp. Immunol. 35:611-19
88. Sakudoh T, Sezutsu H, Nakashima T, Kobayashi I, Fujimoto H, et al. 2007. Carotenoid silk coloration is controlled by a carotenoid-binding protein, a product of the Yellow blood gene. Proc. Natl. Acad. Sci. USA 104:8941-46
89. Sakurai T, Mitsuno H, Haupt SS, Uchino K, Yokohari F, et al. 2011. A single sex pheromone receptor determines chemical response specificity of sexual behavior in the silkmoth Bombyx mori. PLoS Genet. 7:e1002115
90. Sekimoto T, IwamiM, Sakurai S. 2006. Coordinate responses of transcription factors to ecdysone during programmed cell death in the anterior silk gland of the silkworm, Bombyx mori. Insect Mol. Biol. 15:281-92
91. Sekimoto T, Iwami M, Sakurai S. 2007. 20-Hydroxyecdysone regulation of two isoforms of the Ets transcription factor E74 gene in programmed cell death in the silkworm anterior silk gland. Insect Mol. Biol. 16:581-90
92. Shimomura M, Minami H, Suetsugu Y, Ohyanagi H, Satoh C, et al. 2009. KAIKObase: An integrated silkworm genome database and data mining tool. BMC Genomics 10:486
93. Shinoda T, Itoyama K. 2003. Juvenile hormone acid methyltransferase: A key regulatory enzyme for insect metamorphosis. Proc. Natl. Acad. Sci. USA 100:11986-91
94. Shirai H, Kamimura M, Yamaguchi J, Imanishi S, Kojima T, Fujiwara H. 2012. Two adjacent cisregulatory elements are required for ecdysone response of ecdysone receptor (EcR) B1 transcription. PLoS One 7:e49348
95. Suzuki MG, Suzuki K, Aoki F, Ajimura M. 2012. Effect of RNAi-mediated knockdown of the Bombyx mori transformer-2 gene on the sex-specific splicing of Bmdsx pre-mRNA. Int. J. Dev. Biol. 56:693-99
96. Takahasi K, Ochiai M, Horiuchi M, Kumeta H, Ogura K, et al. 2009. Solution structure of the silkworm βGRP/GNBP3 N-terminal domain reveals the mechanism for β-1,3-glucan-specific recognition. Proc. Natl. Acad. Sci. USA 106:11679-84
97. Takasu Y, Hata T, Uchino K, ZhangQ. 2010. Identification of Ser2 proteins asmajor sericin components in the non-cocoon silk of Bombyx mori. Insect Biochem. Mol. Biol. 40:339-44
98. Takasu Y, Kobayashi I, Beumer K, Uchino K, Sezutsu H, et al. 2010. Targeted mutagenesis in the silkworm Bombyx mori using zinc finger nuclease mRNA injection. Insect Biochem. Mol. Biol. 40:759-65
99. Tamura T, Thibert C, Royer C, Kanda T, Abraham E, et al. 2000. Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector. Nat. Biotechnol. 18:81-84
100. Tan A, Tanaka H, Tamura T, Shiotsuki T. 2005. Precocious metamorphosis in transgenic silkworms overexpressing juvenile hormone esterase. Proc. Natl. Acad. Sci. USA 102:11751-56
101. Tanaka H, Ishibashi J, Fujita K, Nakajima Y, Sagisaka A, et al. 2008. A genome-wide analysis of genes and gene families involved in innate immunity of Bombyx mori. Insect Biochem. Mol. Biol. 38:1087-110
102. TatematsuK,Kobayashi I,UchinoK, Sezutsu H, Iizuka T, et al. 2010. Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori. Transgenic Res. 19:473-87
103. Terenius O, Papanicolaou A, Garbutt JS, Eleftherianos I, Huvenne H, et al. 2011. RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design. J. Insect Physiol. 57:231-45
104. Tian L, Guo E, Diao Y, Zhou S, Peng Q, et al. 2010. Genome-wide regulation of innate immunity by juvenile hormone and 20-hydroxyecdysone in the Bombyx fat body. BMC Genomics 11:549
105. Tian L, Guo E, Wang S, Liu S, Jiang RJ, et al. 2010. Developmental regulation of glycolysis by 20- hydroxyecdysone and juvenile hormone in fat body tissues of the silkworm, Bombyx mori. J. Mol. Cell Biol. 2:255-63
106. Tian L, Liu S, Liu H, Li S. 2012. 20-hydroxyecdysone upregulates apoptotic genes and induces apoptosis in the Bombyx fat body. Arch. Insect Biochem. Physiol. 79:207-19
107. Tian L, Ma L, Guo E, Deng X, Ma S, et al. 2013. 20-hydroxyecdysone upregulates Atg genes to induce autophagy in the Bombyx fat body. Autophagy 9:1172-87
108. Tomita M. 2011. Transgenic silkworms that weave recombinant proteins into silk cocoons. Biotechnol. Lett. 33:645-54
109. Uchino K, Imamura M, Shimizu K, Kanda T, Tamura T. 2007. Germ line transformation of the silkworm, Bombyx mori, using the transposable element Minos. Mol. Genet. Genomics 277:213-20
110. Uchino K, Sezutsu H, Imamura M, Kobayashi I, Tatematsu K, et al. 2008. Construction of a piggyBacbased enhancer trap system for the analysis of gene function in silkworm Bombyx mori. Insect Biochem. Mol. Biol. 38:1165-73
111. UhlirovaM, Foy BD, Beaty BJ,Olson KE, Riddiford LM, JindraM. 2003. Use of Sindbis virus-mediated RNA interference to demonstrate a conserved role of Broad-Complex in insect metamorphosis. Proc. Natl. Acad. Sci. USA 100:15607-12
112. Wang F, Xu H, Yuan L, Ma S, Wang Y, et al. 2013. An optimized sericin-1 expression system for mass-producing recombinant proteins in the middle silk glands of transgenic silkworms. Transgenic Res. 22:925-38
113. Wang HB, Iwanaga M, Kawasaki H. 2009. Activation of BMWCP10 promoter and regulation by BR-C Z2 in wing disc of Bombyx mori. Insect Biochem. Mol. Biol. 39:615-23
114. Wang HB, Moriyama M, Iwanaga M, Kawasaki H. 2010. Ecdysone directly and indirectly regulates a cuticle protein gene, BMWCP10, in the wing disc of Bombyx mori. Insect Biochem. Mol. Biol. 40:453-59
115. WangS, Liu S,Liu H,Wang J, Zhou S, et al. 2010. 20-hydroxyecdysone reduces insect food consumption resulting in fat body lipolysis during molting and pupation. J. Mol. Cell Biol. 2:128-38
116. Wang Y, Cheng T, Rayaprolu S, Zou Z, Xia Q, et al. 2007. Proteolytic activation of pro-spätzle is required for the induced transcription of antimicrobial peptide genes in lepidopteran insects. Dev. Comp. Immunol. 31:1002-12
117. Xia Q, Cheng D, Duan J, Wang G, Cheng T, et al. 2007. Microarray-based gene expression profiles in multiple tissues of the domesticated silkworm, Bombyx mori. Genome Biol. 8:R162
118. Xia Q, Guo Y, Zhang Z, Li D, Xuan Z, et al. 2009. Complete resequencing of 40 genomes reveals domestication events and genes in silkworm (Bombyx). Science 326:433-36
119. Xia Q, Xiang Z, eds. 2013. The Genome of Silkworm. Beijing: Science Press
120. Xia Q, Zhou Z, Lu C, Cheng D, Dai F, et al. 2004. A draft sequence for the genome of the domesticated silkworm (Bombyx mori) . Science 306:1937-40
121. Xiang H, Zhu J, Chen Q, Dai F, Li X, et al. 2010. Single base-resolution methylome of the silkworm reveals a sparse epigenomic map. Nat. Biotechnol. 28:516-20
122. Xu HF, Xia QY, Liu C, Cheng TC, Zhao P, et al. 2006. Identification and characterization of piggyBaclike elements in the genome of domesticated silkworm, Bombyx mori. Mol. Genet. Genomics 276:31-40
123. Xu Q, Lu A, Xiao G, Yang B, Zhang J, et al. 2012. Transcriptional profiling ofmidgut immunity response and degeneration in the wandering silkworm, Bombyx mori. PLoS One 7:e43769
124. Yamaguchi Y, Nariya H, Park JH, Inouye M. 2012. Inhibition of specific gene expressions by proteinmediated mRNA interference. Nat. Commun. 3:607
125. Yamamoto K, Narukawa J, Kadono-Okuda K, Nohata J, Sasanuma M, et al. 2006. Construction of a single nucleotide polymorphism linkage map for the silkworm, Bombyx mori, based on bacterial artificial chromosome end sequences. Genetics 173:151-61
126. Yamamoto K, Nohata J, Kadono-Okuda K, Narukawa J, Sasanuma M, et al. 2008. A BAC-based integrated linkage map of the silkworm Bombyx mori. Genome Biol. 9:R21
127. Yang H, Zhou Z, ZhangH, ChenM, Li J, et al. 2010. Shotgun proteomic analysis of the fat body during metamorphosis of domesticated silkworm (Bombyx mori) . Amino Acids 38:1333-42
128. YangW,ChengT,YeM,DengX,YiH, et al. 2011. Functional divergence amongsilkworm antimicrobial peptide paralogs by the activities of recombinant proteins and the induced expression profiles. PLoS One 6:e18109
129. Yasukochi Y, Ashakumary LA, Baba K, Yoshido A, Sahara K. 2006. A second-generation integrated map of the silkworm reveals synteny and conserved gene order between lepidopteran insects. Genetics 173:1319-28
130. Yasukochi Y, Tanaka-Okuyama M, Shibata F, Yoshido A, Marec F, et al. 2009. Extensive conserved synteny of genes between the karyotypes of Manduca sexta and Bombyx mori revealed by BAC-FISH mapping. PLoS One 4:e7465
131. Yoshiyama T, Namiki T, Mita K, Kataoka H, Niwa R. 2006. Neverland is an evolutionally conserved Rieske-domain protein that is essential for ecdysone synthesis and insect growth. Development 133:2565- 74
132. Young SC, Yeh WL, Gu SH. 2012. Transcriptional regulation of the PTTH receptor in prothoracic glands of the silkworm, Bombyx mori. J. Insect Physiol. 58:102-9
133. Yu HS, Shen YH, Yuan GX, Hu YG, Xu HE, et al. 2011. Evidence of selection at melanin synthesis pathway loci during silkworm domestication. Mol. Biol. Evol. 28:1785-99
134. Yu J, Wu FY, Zou FM, Jia JQ, Wang SP, et al. 2012. Identification of ecdysone response elements (EcREs) in the Bombyx mori cathepsin D promoter. Biochem. Biophys. Res. Commun. 425:113-18
135. Yu X, Zhou Q, Cai Y, Luo Q, Lin H, et al. 2009. A discovery of novel microRNAs in the silkworm (Bombyx mori) genome. Genomics 94:438-44
136. Zha X, Xia Q, Duan J, Wang C, He N, Xiang Z. 2009. Dosage analysis of Z chromosome genes using microarray in silkworm, Bombyx mori. Insect Biochem. Mol. Biol. 39:315-21
137. Zhan S, Huang J, Guo Q, Zhao Y, Li W, et al. 2009. An integrated genetic linkage map for silkworms with three parental combinations and its application to the mapping of single genes and QTL. BMC Genomics 10:389
138. Zhang HK, Cao GL, Zhang XR, Wang XJ, Xue RY, Gong CL. 2012. Effects of BmKIT3 R gene transfer on pupal development of Bombyx mori Linnaeus using a Gal4/UAS binary transgenic system. J. Agric. Food Chem. 60:3173-79
139. Zhang P, Aso Y, Yamamoto K, Banno Y, Wang Y, et al. 2006. Proteome analysis of silk gland proteins from the silkworm, Bombyx mori. Proteomics 6:2586-99
140. Zhang QR, Xu WH, Chen FS, Li S. 2005. Molecular and biochemical characterization of juvenile hormone epoxide hydrolase from the silkworm, Bombyx mori. Insect Biochem. Mol. Biol. 35:153-64
141. Zhang S, Xu Y, FuQ, Jia L, Xiang Z,HeN. 2011. Proteomic analysis of larvalmidgut from the silkworm (Bombyx mori) . Comp. Funct. Genomics 2011:876064
142. Zhang X, Xue R, Cao G, Hu X,Wang X, et al. 2012. Effects of egt gene transfer on the development of Bombyx mori. Gene 491:272-77
143. Zhao P, Dong Z, Duan J, Wang G, Wang L, et al. 2012. Genome-wide identification and immune response analysis of serine protease inhibitor genes in the silkworm, Bombyx mori. PLoS One 7:e31168
144. Zhao P, Wang GH, Dong ZM, Duan J, Xu PZ, et al. 2010. Genome-wide identification and expression analysis of serine proteases and homologs in the silkworm Bombyx mori. BMC Genomics 11:405
145. Zhong X, Zhang L, Zou Y, Yi Q, Zhao P, et al. 2012. Shotgun analysis on the peritrophic membrane of the silkworm Bombyx mori. BMB Rep. 45:665-70
146. Zhou L, Chen X, Shao Z, Huang Y, Knight DP. 2005. Effect of metallic ions on silk formation in the mulberry silkworm, Bombyx mori. J. Phys. Chem. B 109:16937-45
147. Zhou W, Gong C, Xue R, Cao G, Cao J, et al. 2012. Germline transformation of the silkworm Bombyx mori L. by sperm-mediated gene transfer. Biol. Reprod. 87:144
148. Zhou Y, Gao L, Shi H, Xia H, Gao L, et al. 2013. Microarray analysis of gene expression profile in resistant and susceptible Bombyx mori strains reveals resistance-related genes to nucleopolyhedrovirus. Genomics 101:256-62