Transgenic silkworms that weave recombinant proteins into silk cocoons

Biotechnology Letters

Volumn 33, Issue 4, 2011, Pages 645-654

  Tomita, Masahiro ^a  

^a Immuno Biological Laboratories Co Ltd   (Japan)

Author keywords

Glycosylation; Recombinant protein; Sericin; Silk gland; Transgenic silkworm

Indexed keywords

AQUEOUS SOLUTIONS; BOMBYX MORI; MASS PRODUCTION; RECOMBINANT PROTEIN; SERICIN; SILK PROTEINS; TRANSGENICS;

ANIMALS; ESTERIFICATION; GENES; GLYCOSYLATION; MACHINE TOOLS; PRODUCTION ENGINEERING; SILK;

PROTEINS;

HYBRID PROTEIN;

ANIMAL; GENETICS; INSECT; METABOLISM; REVIEW; SILK; SILKWORM; TRANSGENIC ANIMAL;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; BOMBYX; PUPA; RECOMBINANT FUSION PROTEINS; SILK;

BOMBYX MORI;

EID: 79952766382     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-010-0498-z     Document Type: Review

References (44)

1. Adachi T, Tomita M, Yoshizato K (2005) Synthesis of prolyl 4-hydroxylase alpha subunit and type IV collagen in hemocytic granular cells of silkworm, Bombyx mori: involvement of type IV collagen in self-defense reaction and metamorphosis. Matrix Biol 24: 136-154.
2. Adachi T, Tomita M, Shimizu K et al (2006) Generation of hybrid transgenic silkworms that express Bombyx mori prolyl-hydroxylase alpha-subunits and human collagens in posterior silk glands: production of cocoons that contained collagens with hydroxylated proline residues. J Biotechnol 126: 205-219.
3. Adachi T, Wang X, Murata T et al (2010) Production of a non-triple helical collagen alpha chain in transgenic silkworms and its evaluation as a gelatin substitute for cell culture. Biotechnol Bioeng 106: 860-870.
4. Altmann F, Schwihla H, Staudacher E et al (1995) Insect cells contain an unusual, membrane-bound β-N-acetylglucosaminidase probably involved in the processing of protein N-glycans. J Biol Chem 270: 17344-17349.
5. Bencúrová M, Hemmer W, Focke-Tejkl M (2004) Specificity of IgG and IgE antibodies against plant and insect glycoprotein glycans determined with artificial glycoforms of human transferrin. Glycobiology 14: 457-466.
6. Bole DG, Hendershot LM, Kearney JF (1986) Posttranslational association of immunoglobulin heavy chain binding protein with nascent heavy chains in nonsecreting and secreting hybridomas. J Cell Biol 102: 1558-1566.
7. Cary LC, Goebel M, Corsaro BG et al (1989) Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses. Virology 172: 156-169.
8. Choudary PV, Kamita SG, Maeda S (1995) Expression of foreign genes in Bombyx mori larvae using baculovirus vectors. Methods Mol Biol 39: 243-264.
9. Dyck MK, Lacroix D, Pothier F et al (2003) Making recombinant proteins in animals-different systems, different applications. Trends Biotechnol 21: 394-399.
10. Giddings G, Allison G, Brooks D et al (2000) Transgenic plants as factories for biopharmaceuticals. Nat Biotechnol 18: 1151-1155.
11. Gomord V, Chamberlain P, Jefferis R et al (2005) Biopharmaceutical production in plants: problems, solutions and opportunities. Trends Biotechnol 23: 559-565.
12. Grzelak K (1995) Control of expression of silk protein genes. Comp Biochem Physiol B Biochem Mol Biol 110: 671-681.
13. Harvey AJ, Speksnijder G, Baugh LR et al (2002) Expression of exogenous protein in the egg white of transgenic chickens. Nat Biotechnol 20: 396-399.
14. Hendershot L, Bole D, Köhler G et al (1987) Assembly and secretion of heavy chains that do not associate posttranslationally with immunoglobulin heavy chain-binding protein. J Cell Biol 104: 761-767.
15. Hino R, Tomita M, Yoshizato K (2006) The generation of germline transgenic silkworms for the production of biologically active recombinant fusion proteins of fibroin and human basic fibroblast growth factor. Biomaterials 27: 5715-5724.
16. Houdebine LM (2000) Transgenic animal bioreactors. Transgenic Res 9: 305-320.
17. Iizuka M, Tomita M, Shimizu K et al (2008) Translational enhancement of recombinant protein synthesis in transgenic silkworms by a 5′-untranslated region of polyhedrin gene of Bombyx mori Nucleopolyhedrovirus. J Biosci Bioeng 105: 595-603.
18. Iizuka M, Ogawa S, Takeuchi A et al (2009) Production of a recombinant mouse monoclonal antibody in transgenic silkworm cocoons. FEBS J 276: 5806-5820.
19. Inoue S, Tanaka K, Arisaka F et al (2000) Silk fibroin of Bombyx mori is secreted, assembling a high molecular mass elementary unit consisting of H-chain, L-chain, and P25, with a 6: 6: 1 molar ratio. J Biol Chem 275: 40517-40528.
20. Kojima K, Kuwana Y, Sezutsu H et al (2007) A new method for the modification of fibroin heavy chain protein in the transgenic silkworm. Biosci Biotechnol Biochem 71: 2943-2951.
21. Kubelka V, Altmann F, Kornfeld G et al (1994) Structures of the N-linked oligosaccharides of the membrane glycoproteins from three lepidopteran cell lines (Sf-21, IZD-Mb-0503, Bm-N). Arch Biochem Biophys 308: 148-157.
22. Kulakosky PC, Hughes PR, Wood HA (1998) N-linked glycosylation of a baculovirus-expressed recombinant glycoprotein in insect larvae and tissue culture cells. Glycobiology 8: 741-745.
23. Kurihara H, Sezutsu H, Tamura T et al (2007) Production of an active feline interferon in the cocoon of transgenic silkworms using the fibroin H-chain expression system. Biochem Biophys Res Commun 20: 976-980.
24. Lillico SG, McGrew MJ, Sherman A et al (2005) Transgenic chickens as bioreactors for protein-based drugs. Drug Discov Today 10: 191-196.
25. Maeda S, Kawai T, Obinata M et al (1985) Production of human alpha-interferon in silkworm using a baculovirus vector. Nature 315: 592-594.
26. Michaille JJ, Garel A, Prudhomme JC (1990) Cloning and characterization of the highly polymorphic Ser2 gene of Bombyx mori. Gene 86: 177-184.
27. Ogawa S, Tomita M, Shimizu K et al (2007) Generation of a transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon: production of recombinant human serum albumin. J Biotechnol 128: 531-544.
28. Okamoto H, Ishikawa E, Suzuki Y (1982) Structural analysis of sericin genes. Homologies with fibroin gene in the 5′ flanking nucleotide sequences. J Biol Chem 257: 15192-15199.
29. Perdrix-Gillot S (1979) DNA synthesis and endomitoses in the giant nuclei of the silkgland of Bombyx mori. Biochimie 61: 171-204.
30. Royer C, Jalabert A, Da Rocha M et al (2005) Biosynthesis and cocoon-export of a recombinant globular protein in transgenic silkworms. Transgenic Res 14: 463-472.
31. Rubin GM, Spradling AC (1982) Genetic transformation of Drosophila with transposable element vectors. Science 218: 348-353.
32. Shields RL, Lai J, Keck R et al (2002) Lack of fucose on human IgG1 N-linked oligosaccharide improves binding to human FccRIII and antibodydependent cellular toxicity. J Biol Chem 277: 26733-26740.
33. Shimizu K, Ogawa S, Hino R et al (2007) Structure and function of 5′-flanking regions of Bombyx mori fibroin heavy chain gene: identification of a novel transcription enhancing element with a homeodomain protein-binding motif. Insect Biochem Mol Biol 37: 713-725.
34. Shinkawa T, Nakamura K, Yamane N et al (2003) The absence of fucose but not the presence of galactose or bisecting N-acetylglucosamine of human IgG1 complex-type oligosaccharides shows the critical role of enhancing antibody-dependent cellular cytotoxicity. J Biol Chem 278: 3466-3473.
35. Staudacher E, Kubelka V, März L (1992) Distinct N-glycan fucosylation potentials of three lepidopteran cell lines. Eur J Biochem 207: 987-993.
36. Takasu Y, Yamada H, Tamura T et al (2007) Identification and characterization of a novel sericin gene expressed in the anterior middle silk gland of the silkworm Bombyx mori. Insect Biochem Mol Biol 37: 1234-1240.
37. Tamura T, Thibert C, Royer C et al (2000) Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector. Nat Biotechnol 18: 81-84.
38. Tatematsu K, Kobayashi I, Uchino K et al (2010) Construction of a binary transgenic gene expression system for recombinant protein production in the middle silk gland of the silkworm Bombyx mori. Transgenic Res 19: 473-487.
39. Tomita M, Munetsuna H, Sato T et al (2003) Transgenic silkworms produce recombinant human type III procollagen in cocoons. Nat Biotechnol 21: 52-56.
40. Tomita M, Hino R, Ogawa S et al (2007) A germline transgenic silkworm that secretes recombinant proteins in the sericin layer of cocoon. Transgenic Res 16: 449-465.
41. Urano S, Kaneko C, Nei T et al (2010) A cell-free assay to estimate the neutralizing capacity of granulocyte-macrophage colony-stimulating factor autoantibodies. J Immunol Methods 360: 141-148.
42. Yanagisawa S, Zhu Z, Kobayashi I et al (2007) Improving cell-adhesive properties of recombinant Bombyx mori silk by incorporation of collagen or fibronectin derived peptides produced by transgenic silkworms. Biomacromolecules 8: 3487-3492.
43. Zhao A, Zhao T, Zhang Y et al (2010) New and highly efficient expression systems for expressing selectively foreign protein in the silk glands of transgenic silkworm. Transgenic Res 19: 29-44.
44. Zhu Z, Kikuchi Y, Kojima K et al (2010) Mechanical properties of regenerated Bombyx mori silk fibers and recombinant silk fibers produced by transgenic silkworms. J Biomater Sci Polym Ed 21: 395-411.