Germline transformation of the silkworm Bombyx mori L. using a piggyBac transposon-derived vector

Nature Biotechnology

Volumn 18, Issue 1, 2000, Pages 81-84

  Toshiki, Tamura ^a   Chantal, Thibert ^b   Corinne, Royer ^c   Toshio, Kanda ^a   Eappen, Abraham ^a   Mari, Kamba ^a   Natuo, Kômoto ^a   Jean Luc, Thomas ^c   Bernard, Mauchamp ^c   Gérard, Chavancy ^c   Paul, Shirk ^d   Malcolm, Fraser ^e   Jean Claude, Prudhomme ^b   Pierre, Couble ^b  

^a NATIONAL INSTITUTE OF AGROBIOLOGICAL SCIENCES   (Japan)

^b CNRS   (France)

^c INRA   (France)

^d CENTER FOR MEDICAL   (United States)

^e UNIVERSITY OF NOTRE DAME   (United States)

Author keywords

Bombyx; PiggyBac; Transgenesis; Transposon

Indexed keywords

ACTIN; GREEN FLUORESCENT PROTEIN; TRANSPOSASE;

ANIMAL EXPERIMENT; ARTICLE; CONTROLLED STUDY; GENE TRANSFER; GENETIC ANALYSIS; GENETIC TRANSFORMATION; LEPIDOPTERA; NONHUMAN; PLASMID; PRIORITY JOURNAL; PROMOTER REGION; REPORTER GENE; SILKWORM; TRANSPOSON;

ACTINS; AGING; ANIMALS; ANIMALS, GENETICALLY MODIFIED; BASE SEQUENCE; BOMBYX; CROSSES, GENETIC; DNA TRANSPOSABLE ELEMENTS; FEMALE; GENETIC VECTORS; GERM-LINE MUTATION; GREEN FLUORESCENT PROTEINS; LARVA; LUMINESCENT PROTEINS; MALE; MOTHS; MUTAGENESIS, SITE-DIRECTED; PROMOTER REGIONS (GENETICS); PUPA; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS, DNA; TERMINAL REPEAT SEQUENCES; TRANSFORMATION, GENETIC; TRANSGENES; TRANSPOSASES;

ANIMALIA; BOMBYX; BOMBYX MORI; INSECTA; INVERTEBRATA; LEPIDOPTERA; TRICHOPLUSIA NI;

EID: 0033988156     PISSN: 10870156     EISSN: None     Source Type: Journal    
DOI: 10.1038/71978     Document Type: Article

References (17)

1. Rubin, G.M. & Spradling, A.C. Genetic transformation of Drosophila with transposable element vectors. Science 218, 348-353 (1982).
2. O'Brochta, D.A. & Atkinson, P.W. Transposable elements and gene transformation in non-drosophilid insects. Insect Biochem. Mol. Biol. 26, 739-753 (1996).
3. Loukeris, T.G., Livadaras, I., Arca, B., Zabalou, S. & Savakis C. Gene transfer into the medfly, Ceratitis capitata, with a Drosophila hydei transposable element. Science 270, 2002-2005 (1995).
4. Handler, A.M., MacCombs, S.D., Fraser, M.J. & Saul, S.H. The lepidopteran transposon vector, piggyBac, mediates germ-line transformation in the mediterranean fruit fly. Proc. Natl. Acad. Sci. USA. 95, 7520-7525 (1998).
5. Coates, C.J., Jasinskiene, N., Miyashiro, L. & James, A.A. Mariner transposition and transformation of the yellow fever mosquito, Aedes aegypti. Proc. Natl. Acad. Sci. USA 95, 3748-3751 (1998).
6. Jasinskiene, N. et al. Stable transformation of the yellow fever mosquito Aedes aegypti, with the hermes element from the housefly. Proc. Natl. Acad. Sci. USA 95, 3743-3747 (1998).
7. Lozovskaya, E.R., Nurminsky, D.I., Hartl, D.L. & Sullivan, D.T. Germline transformation of Drosophila virilis mediated by the transposable element hobo. Genetics 142, 173-177 (1995).
8. Lohe, A.R. & Hartl, D.L. Germline transformation of Drosophila virilis with the transposable element mariner. Genetics 143, 365-374 (1996).
9. Gomez, S.P. & Handler, A.M. A Drosophila melanogaster hobo-white (+) vector mediates low frequency gene transfer in D. virilis with full interspecific white (+) complementation. Insect Mol. Biol. 6, 165-171 (1997).
10. Nagaraju, J., Klymenko, V. & Couble, P. In Encyclopedia of genetics (ed. Reeves, E.) (Fitzroy Dearborn, London, UK; In press).
11. Yamao, M. et al. Gene targeting in the silkworm by use of a baculovirus. Genes Dev. 13, 511-516 (1999).
12. Cary, L.C. et al. Transposon mutagenesis of baculoviruses: analysis of Trichoplusia ni transposon IFP2 insertions within the FP-locus of nuclear polyhedrosis viruses. Virology 172, 156-69 (1989).
13. Wang, H.H. & Fraser, M.J. TTAA serves as the target site for the TFP3 lepidopteran transposon insertions in both nuclear polyhedrosis virus and Trichoplusia ni genomes. Insect Mol Biol. 1, 109-116 (1993).
14. Robertson, H.M. & Asplund, M.L. Bmmar1: a basal lineage of the mariner family of transposable elements in the silkworm moth Bombyx mori. Insect Biochem. Mol. Biol. 26, 945-954 (1996).
15. Mounier, N. & Prudhomme, J.C. Differential expression of muscle and cytoplasmic actin genes during development of Bombyx mori. Insect Biochem. 21, 523-533 (1991).
16. Mangé, A., Julien, E., Prudhomme, J.C. & Couble, P. A strong inhibitory element down-regulates SRE-stimulated transcription of the A3 cytoplasmic actin gene of Bombyx mori. J. Mol. Biol. 265, 266-274 (1997).
17. Kanda, T. & Tamura, T. Microinjection system into the early embryo of the silkworm, Bombyx mori, by using air pressure. Bulliten of the National Institute of Sericultural and Entomological Science 2, 32-46 (1991).