Critical microcarrier properties affecting the expansion of undifferentiated human embryonic stem cells

Stem Cell Research

Volumn 7, Issue 2, 2011, Pages 97-111

  Chen, Allen Kuan Liang ^a   Chen, Xiaoli ^a   Choo, Andre Boon Hwa ^a   Reuveny, Shaul ^b   Oh, Steve Kah Weng ^a  

^a AGENCY FOR SCIENCE   (Singapore)

^b ISRAEL INSTITUTE FOR BIOLOGICAL RESEARCH   (Israel)

Author keywords

[No Author keywords available]

Indexed keywords

CELLULOSE; LAMININ; MATRIGEL;

ARTICLE; CELL CULTURE; CELL EXPANSION; CELL GROWTH; CELL VIABILITY; CONTROLLED STUDY; EMBRYONIC STEM CELL; HUMAN; HUMAN CELL; PLURIPOTENT STEM CELL; PRIORITY JOURNAL;

CELL CULTURE TECHNIQUES; CELL GROWTH PROCESSES; CELL LINE; CULTURE MEDIA; EMBRYONIC STEM CELLS; HUMANS; VIRUS REPLICATION;

MAMMALIA;

EID: 79960288995     PISSN: 18735061     EISSN: 18767753     Source Type: Journal    
DOI: 10.1016/j.scr.2011.04.007     Document Type: Article

References (38)

1. Bajpai R., Lesperance J., Kim M., Terskikh A.V. Efficient propagation of single cells accutase-dissociated human embryonic stem cells. Mol. Reprod. Dev. 2008, 75:818-827.
2. Beattie G.M., Lopez A.D., Bucay N., Hinton A., Firpo M.T., King C.C., Hayeka A. Activin A maintains pluripotency of human embryonic stem cells in the absence of feeder layers. Stem Cells 2005, 23:489-495.
3. Bissell D.M., Arenson D.M., Maher J.J., Roll F.J. Support of cultured hepatocytes by a laminin-rich gel. Evidence for a functionally significant subendothelial matrix in normal rat liver. J. Clin. Invest. 1987, 79:801.
4. Braam S.R., Zeinstra L., Litjens S., Oostwaard D.W.-v., van den Brink S., van Laake L., Lebrin F., Kats P., Hochstenbach R., Passier R., Sonnenberg A., Mummery C.L. Recombinant vitronectin is a functionally defined substrate that supports human embryonic stem cell self-renewal via alphavbeta5 integrin. Stem Cells 2008, 26:2257-2265.
5. Chan K.K.-K., Wu S.M., Nissom P.M., Oh S.K.W., Choo A.B.H. Generation of high-level stable transgene expressing human embryonic stem cell lines using Chinese hamster elongation factor-1? promoter system. Stem Cells Dev. 2008, 17:825-836.
6. Chen A.K., Chen X., Choo A.B., Reuveny S., Oh S.K. Expansion of Human Embryonic Stem Cells on Cellulose Microcarriers 2010, John Wiley & Sons, Inc.
7. Chin A.C.P., Fong W.J., Goh L.-T., Philp R., Oh S.K.W., Choo A.B.H. Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J. Biotechnol. 2007, 130:320-328.
8. Chin A.C.P., Padmanabhan J., Oh S.K.W., Choo A.B.H. Defined and serum-free media support undifferentiated human embryonic stem cell growth. Stem Cell Dev. 2010, 19:753-761.
9. Choo A., Padmanabhan J., Chin A., Fong W.J., Oh S.K.W. Immortalized feeders for the scale-up of human embryonic stem cells in feeder and feeder-free conditions. J. Biotechnol. 2006, 122:130-141.
10. Choo A.B., Tan H.L., Ang S.N., Fong W.J., Chin A., Lo J., Zheng L., Hentze H., Philp R.J., Oh S.K.W., Yap M. Selection against undifferentiated human embryonic stem cells by a cytotoxic antibody recognizing podocalyxin-like protein-1. Stem Cells 2008, 26:1454-1463.
11. Chu L., Robinson D.K. Industrial choices for protein production by large-scale cell culture. Curr. Opin. Biotechnol. 2001, 12:180-187.
12. Ellerström C., Strehl R., Noaksson K., Hyllner J., Semb H. Facilitated expansion of human embryonic stem cells by single-cell enzymatic dissociation. Stem Cells 2007, 25:1690-1696.
13. Hasegawa K., Fujioka T., Nakamura Y., Nakatsuji N., Suemori H. A Method for the selection of human embryonic stem cell sublines with high replating efficiency after single-cell dissociation. Stem Cells 2006, 24:2649-2660.
14. Kehoe D.E., Jing D., Lock L.T., Tzanakakis E.S. Scalable stirred-suspension bioreactor culture of human pluripotent stem cells. Tissue Eng. Part A 2010, 16:405-421.
15. Kleinman H.K., McGarvey M.L., Liotta L.A., Robey P.G., Tryggvason K., Martin G.R. Isolation and characterization of type IV procollagen, laminin, and heparan sulfate proteoglycan from the EHS sarcoma. Biochemistry (Mosc.) 1982, 21:6188-6193.
16. Lecina M., Ting S., Choo A., Reuveny S., Oh S. Scalable platform for hESC differentiation to cardiomyocytes in suspended microcarrier cultures. Tissue Eng. Part C Methods 2010, 16:1609-1619.
17. Leung H.W., Chen A., Choo A., Oh S. Agitation can induce differentiation of human pluripotent stem cells in microcarrier cultures. Tissue Eng. Part C Methods 2010, 17:165-172.
18. Lock L.T., Tzanakakis E.S. Expansion and differentiation of human embryonic stem cells to endoderm progeny in a microcarrier stirred-suspension culture. Tissue Eng. Part A 2009, 15:2051-2063.
19. Malda J., Frondoza C.G. Microcarriers in the engineering of cartilage and bone. Trends Biotechnol. 2006, 24:299-304.
20. Mukhopadhyay A., Mukhopadhyay S.N., Talwar G.P. Influence of serum proteins on the kinetics of attachment of vero cells to cytodex microcarriers. J. Chem. Technol. Biotechnol. 1993, 56:369-374.
21. Mummery C.L. Cardiology: solace for the broken-hearted?. Nature 2005, 433:585-587.
22. Nie Y., Bergendahl V., Hei D.J., Jones J.M., Palecek S.P. Scalable culture and cryopreservation of human embryonic stem cells on microcarriers. Biotechnol. Prog. 2009, 25:20-31.
23. Oh S.K.W., Chen A.K., Mok Y., Chen X., Lim U.-M., Chin A., Choo A.B.H., Reuveny S. Long-term microcarrier suspension cultures of human embryonic stem cells. Stem Cell Res. 2009, 2:219-230.
24. Phillips B.W., Horne R., Lay T.S., Rust W.L., Teck T.T., Crook J.M. Attachment and growth of human embryonic stem cells on microcarriers. J. Biotechnol. 2008, 138:24-32.
25. Rao B.M., Zandstra P.W. Culture development for human embryonic stem cell propagation: molecular aspects and challenges. Curr. Opin. Biotechnol. 2005, 16:568-576.
26. Reubinoff B.E., Pera M.F., Fong C.-Y., Trounson A., Bongso A. Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro. Nat. Biotechnol. 2000, 18:399-404.
27. Reuveny S. Microcarrier culture systems. Large-Scale Mammalian Cell Culture Technology 1990, 271-341. Marcel Dekker, Inc., New York. A.S. Lubiniecki (Ed.).
28. Reuveny S., Bino T., Rosenberg H., Mizrahi A. A new cellulose-based microcarrier culturing system. Dev. Biol. Stand. 1980, 46:137-145.
29. Reuveny S., Mizrahi A., Kotler M., Freeman A. Factors affecting cell attachment, spreading, and growth on derivatized microcarriers. I. Establishment of working system and effect of the type of the amino-charged groups. Biotechnol. Bioeng. 1983, 25:469-480.
30. Rodin S., Domogatskaya A., Ström S., Hansson E.M., Chien K.R., Inzunza J., Hovatta O., Tryggvason K. Long-term self-renewal of human pluripotent stem cells on human recombinant laminin-511. Nat. Biotechnol. 2010, 28:611-615.
31. Skottman H., Hovatta O. Culture conditions for human embryonic stem cells. Reproduction 2006, 132:691-698.
32. Storm M.P., Orchard C.B., Bone H.K., Chaudhuri J.B., Welham M.J. Three-dimensional culture systems for the expansion of pluripotent embryonic stem cells. Biotechnol. Bioeng. 2010, 107:683-695.
33. Thomas R.J., Anderson D., Chandra A., Smith N.M., Young L.E., Williams D., Denning C. Automated, scalable culture of human embryonic stem cells in feeder-free conditions. Biotechnol. Bioeng. 2009, 102:1636-1644.
34. Thomson J.A., Itskovitz-Eldor J., Shapiro S.S., Waknitz M.A., Swiergiel J.J., Marshall V.S., Jones J.M. Embryonic stem cell lines derived from human blastocysts. Science 1998, 282:1145-1147.
35. van Wezel A.L. Growth of cell-strains and primary cells on microcarriers in homogeneous culture. Nature 1967, 216:64-65.
36. Veraitch F.S., Scott R., Wong J.-W., Lye G.J., Mason C. The impact of manual processing on the expansion and directed differentiation of embryonic stem cells. Biotechnol. Bioeng. 2008, 99:1216-1229.
37. Xu C., Inokuma M.S., Denham J., Golds K., Kundu P., Gold J.D., Carpenter M.K. Feeder-free growth of undifferentiated human embryonic stem cells. Nat. Biotechnol. 2001, 19:971-974.
38. Yap L.Y.W., Li J., Ong I.Y.P.L.T., Ow J.a.Z.-E., Goh J.C.H., Nurcombe J.C.H., Hobley J., Choo A.B.H., Oh S.K.W., Cool S.M., Birch W.R. Defining a Threshold Surface Density of Vitronectin for the Stable Expansion of Human Embryonic Stem Cells. Tissue Eng. Part C Methods 2011, 17:193-207.