Reprogramming of human somatic cells to pluripotency with defined factors

Nature

Volumn 451, Issue 7175, 2008, Pages 141-146

  Park, In Hyun ^a,b,c,d   Zhao, Rui ^a,b,c,d   West, Jason A ^a,b,c,d   Yabuuchi, Akiko ^a,b,c,d   Huo, Hongguang ^a,b,c,d   Ince, Tan A ^a   Lerou, Paul H ^a   Lensch, M William ^a,b,c,d   Daley, George Q ^a,b,c,d  

^a BOSTON CHILDREN S HOSPITAL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c BRIGHAM AND WOMEN S HOSPITAL   (United States)

^d HARVARD STEM CELL INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

KRUPPEL LIKE FACTOR 4; MYC PROTEIN; OCTAMER TRANSCRIPTION FACTOR 4; TRANSCRIPTION FACTOR SOX2;

EMBRYO; GENE EXPRESSION; HOMINID; MOLECULAR ANALYSIS; RESEARCH WORK; SKIN;

ARTICLE; CELL DIFFERENTIATION; CELL STRUCTURE; CONTROLLED STUDY; CYTOLOGY; EMBRYO; EMBRYONIC STEM CELL; FETUS; FIBROBLAST; GENE EXPRESSION; HUMAN; HUMAN CELL; NEWBORN; PLURIPOTENT STEM CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; SKIN FIBROBLAST; SOMATIC CELL;

ADULT; ANIMALS; CELL DIFFERENTIATION; CELL SHAPE; CELLS, CULTURED; DNA METHYLATION; DNA-BINDING PROTEINS; EMBRYONIC STEM CELLS; FETUS; FIBROBLASTS; GENE EXPRESSION PROFILING; HMGB PROTEINS; HOMEODOMAIN PROTEINS; HUMANS; INFANT, NEWBORN; KRUPPEL-LIKE TRANSCRIPTION FACTORS; MICE; OCTAMER TRANSCRIPTION FACTOR-3; PLURIPOTENT STEM CELLS; PROMOTER REGIONS (GENETICS); PROTO-ONCOGENE PROTEINS C-MYC; TERATOMA; TRANSCRIPTION FACTORS; TRANSPLANTATION, HETEROLOGOUS;

IPS; MURINAE; MUS;

EID: 38049187707     PISSN: 00280836     EISSN: 14764679     Source Type: Journal    
DOI: 10.1038/nature06534     Document Type: Article

References (24)

1. Wakayama, T. et al. Differentiation of embryonic stem cell lines generated from adult somatic cells by nuclear transfer. Science 292, 740-743 (2001).
2. Cowan, C. A., Atienza, J., Melton, D. A. & Eggan, K. Nuclear reprogramming of somatic cells after fusion with human embryonic stem cells. Science 309, 1369-1373 (2005).
3. Kanatsu-Shinohara, M. et al. Generation of pluripotent stem cells from neonatal mouse testis. Cell 119, 1001-1012 (2004).
4. Takahashi, K. & Yamanaka, S. Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors. Cell 126, 663-676 (2006).
5. Wernig, M. et al. In vitro reprogramming of fibroblasts into a pluripotent ES-cell-like state. Nature 448, 318-324 (2007).
6. Okita, K., Ichisaka, T. & Yamanaka, S. Generation of germline-competent induced pluripotent stem cells. Nature 448, 313-317 (2007).
7. Maherali, N. et al. Directly reprogrammed fibroblasts show global epigenetic remodeling and widespread tissue contribution. Cell Stem Cell 1, 55-70 (2007).
8. Zwaka, T. P. & Thomson, J. A. Homologous recombination in human embryonic stem cells. Nature Biotechnol. 21, 319-321 (2003).
9. Watanabe, K. et al. A ROCK inhibitor permits survival of dissociated human embryonic stem cells. Nature Biotechnol. 25, 681-686 (2007).
10. Meissner, A., Wernig, M. & Jaenisch, R. Direct reprogramming of genetically unmodified fibroblasts into pluripotent stem cells. Nature Biotechnol. 25, 1177-1181 (2007).
11. Blelloch, R., Venere, M., Yen, J. & Ramalho-Santos, M. Generation of induced pluripotent stem cells in the absence of drug selection. Cell Stem Cell 1, 245-247 (2007).
12. Bodnar, A. G. et al. Extension of life-span by introduction of telomerase into normal human cells. Science 279, 349-352 (1998).
13. Hahn, W. C. et al. Creation of human tumour cells with defined genetic elements. Nature 400, 464-468 (1999).
14. Adewumi, O. et al. Characterization of human embryonic stem cell lines by the International Stem Cell Initiative. Nature Biotechnol. 25, 803-816 (2007).
15. Tada, M., Takahama, Y., Abe, K., Nakatsuji, N. & Tada, T. Nuclear reprogramming of somatic cells by in vitro hybridization with ES cells. Curr. Biol. 11, 1553-1558 (2001).
16. Lensch, M. W., Schlaeger, T. M., Zon, L. I. & Daley, G. Q. Teratoma formation assays with human embryonic stem cells: a rationale for one type of human-animal chimera. Cell Stem Cell 1, 253-258 (2007).
17. Lensch, M. W. & Ince, T. A. The terminology of teratocarcinomas and teratomas. Nature Biotechnol. 25, 1211 (2007).
18. Xu, C., Police, S., Rao, N. & Carpenter, M. K. Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells. Circ. Res. 91, 501-508 (2002).
19. Takahashi, K. et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872 (2007).
20. Yu, J. et al. Induced pluripotent stem cell lines derived from human somatic cells. Science. doi:10.1126/science.1151526 (20 November 2007).
21. Van Parijs, L. et al. Uncoupling IL-2 signals that regulate T cell proliferation, survival, and Fas-mediated activation-induced cell death. Immunity 11, 281-288 (1999).
22. Eischen, C. M., Roussel, M. F., Korsmeyer, S. J. & Cleveland, J. L. Bax loss impairs Myc-induced apoptosis and circumvents the selection of p53 mutations during Myc-mediated lymphomagenesis. Mol. Cell. Biol. 21, 7653-7662 (2001).
23. Deb-Rinker, P., Ly, D., Jezierski, A., Sikorska, M. & Walker, P. R. Sequential DNA methylation of the Nanog and Oct-4 upstream regions in human NT2 cells during neuronal differentiation. J. Biol. Chem. 280, 6257-6260 (2005).
24. Freberg, C. T., Dahl, J. A., Timoskainen, S. & Collas, P. Epigenetic reprogramming of OCT4 and NANOG regulatory regions by embryonal carcinoma cell extract. Mol. Biol. Cell 18, 1543-1553 (2007).