High-level expression of glutaryl-7-aminocephalosporanic acid acylase from Pseudomonas diminuta NK703 in Escherichia coli by combined optimization strategies

Journal of Biotechnology

Volumn 168, Issue 4, 2013, Pages 607-615

  Ma, Xiaoqiang ^a   Su, Erzheng ^a,b   Zhu, Ying ^a   Deng, Senwen ^a   Wei, Dongzhi ^a  

^a EAST CHINA UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b NANJING FORESTRY UNIVERSITY   (China)

Author keywords

Glutaryl 7 aminocephalosporanic acid acylase (GLA); Lactose induction; Pseudomonas diminuta; Soluble expression

Indexed keywords

GLUTARYL-7-AMINOCEPHALOSPORANIC ACID ACYLASE; HIGH LEVEL EXPRESSION; LACTOSE INDUCTION; OPTIMIZATION STRATEGY; OPTIMIZED CONDITIONS; PSEUDOMONAS DIMINUTA; SOLUBLE EXPRESSION; TOTAL SOLUBLE PROTEIN;

BACTERIA; CLONING; FERMENTERS; OIL FIELDS; OPTIMIZATION; SUGARS;

ESCHERICHIA COLI;

7 AMINOCEPHALOSPORANIC ACID; DEXTRIN; LACTOSE; PROTEIN;

ARTICLE; BIOREACTOR; BREVUNDIMONAS DIMINUTA; CARBON SOURCE; CULTURE MEDIUM; ESCHERICHIA COLI; FEEDING; GENE EXPRESSION SYSTEM; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SCALE UP; SOLUBILITY;

BREVUNDIMONAS DIMINUTA; ESCHERICHIA COLI;

GLUTARYL-7-AMINOCEPHALOSPORANIC ACID ACYLASE (GLA); LACTOSE INDUCTION; PSEUDOMONAS DIMINUTA; SOLUBLE EXPRESSION;

CEPHALOSPORINS; CULTURE MEDIA; ESCHERICHIA COLI; FERMENTATION; GENE EXPRESSION REGULATION, BACTERIAL; LACTOSE; PENICILLIN AMIDASE; PSEUDOMONAS;

EID: 84888820418     PISSN: 01681656     EISSN: 18734863     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2013.08.024     Document Type: Article

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