Cloning and high expression of glutaryl 7-aminocephalosporanic acid acylase gene from Pseudomonas diminuta

Biotechnology Letters

Volumn 23, Issue 13, 2001, Pages 1067-1071

  Kim, Dae Weon ^a   Yoon, Ki Hong ^a  

^a Woosong University   (South Korea)

Author keywords

Expression; Glutaryl 7 ACA acylase; Nucleotide sequence; Pseudomonas diminuta

Indexed keywords

AMIDASE; BACTERIAL ENZYME; GLUTARYL 7 AMINOCEPHALOSPORANIC ACID ACYLASE; SIGNAL PEPTIDE; UNCLASSIFIED DRUG;

ARTICLE; BACTERIAL GROWTH; ENZYME ACTIVATION; ESCHERICHIA COLI; GENE CONSTRUCT; GENE DELETION; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; PLASMID; PROTEIN PROCESSING; PSEUDOMONAS DIMINUTA;

EID: 0034903834     PISSN: 01415492     EISSN: None     Source Type: Journal    
DOI: 10.1023/A:1010554323405     Document Type: Article

References (14)

1. Basic local alignment search tool
2. Cloning and nucleotide sequence of a novel 7β-(4-carboxybutamamido) cephalosporanic acid acylase gene of Bacillus laterosoporus and its expression in Escherichia coli and Bacillus subtilis
3. The isolation and kinetics of penicillin amidase from E. coli
4. Isolation and characterization of a Pseudomonas strain producing glutaryl 7-aminocephalo sporanic acid acylase
5. Reconstitution in vivo of penicillin G acylase activity from separately expressed subunits
6. High-level production, chemical modification and site-directed mutagenesis of a cephalosporin C acylase from Pseudomonas strain N176
7. A novel 7-β-(4-carboxybutanamido)cephalosporanic acid acylase isolated from Pseudomnas strain C427 and its high-level production in Escherichia coli
8. Isolation and characterization of a novel soil strain, Pseudomonas cepacia BY21, with glutaryl-7-aminocephalosporanic acid acylase activity
9. Isolation of novel Pseudomonas diminuta KAC-1 strain producing glutaryl 7-aminocephalosporanic acid acylase
10. The 2.0 A crystal structure of cephalosporin acylase
11. Cloning and characterization of GL 7-ACA acylase gene from Pseudomonas sp. GK16
12. In vivo post-translational processing and subunit reconstitution of cephalosporin acylase from Pseudomonas sp. 130
13. Overproduction and purification of glutaryl 7-aminocephalosporanic acid acylase
14. Molecular aspects of penicillin and cephalosporin acylases