Overproduction of alkaline polygalacturonate lyase in recombinant Escherichia coli by a two-stage glycerol feeding approach

Bioresource Technology

Volumn 102, Issue 22, 2011, Pages 10671-10678

  Fang, Shuying ^a   Li, Jianghua ^a   Liu, Long ^a   Du, Guocheng ^a   Chen, Jian ^a  

^a JIANGNAN UNIVERSITY   (China)

Author keywords

Alkaline polygalacturonate lyase; Bacillus subtilis; Escherichia coli; Extracellular expression

Indexed keywords

BACILLUS SUBTILIS; CODING GENES; E. COLI; EXPRESSION SYSTEM; EXTRACELLULAR; FEEDING RATE; FEEDING STRATEGIES; INDUCTION TEMPERATURES; MEDIA COMPOSITION; POLYGALACTURONATES; RECOMBINANT E. COLI; RECOMBINANT ESCHERICHIA COLI; SPECIFIC GROWTH RATE; TWO STAGE;

BACILLI; BACTERIOLOGY; ESCHERICHIA COLI; FEEDING; GENES; GROWTH KINETICS;

GLYCEROL;

ALKALINE POLYGALACTURONATE LYASE; GLYCEROL; ISOPROPYL THIOGALACTOSIDE; PECTATE LYASE; UNCLASSIFIED DRUG;

ALCOHOL; CHEMICAL COMPOSITION; COLIFORM BACTERIUM; CYTOLOGY; ENZYME ACTIVITY; FERMENTATION; FOOD SUPPLEMENTATION; GENE EXPRESSION; GROWTH RATE; MICROBIAL ACTIVITY;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL CELL; BACTERIAL GENE; BIOREACTOR; CELL GROWTH; ENZYME SYNTHESIS; ESCHERICHIA COLI; FEEDING; GENE EXPRESSION; GENETIC CODE; GROWTH RATE; NONHUMAN; PRIORITY JOURNAL; PROMOTER REGION; TEMPERATURE;

ALKALIES; BACILLUS SUBTILIS; BATCH CELL CULTURE TECHNIQUES; BIOMASS; BIOTECHNOLOGY; CARBON; CULTURE MEDIA; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; GENE EXPRESSION; GENES, BACTERIAL; GLYCEROL; ISOPROPYL THIOGALACTOSIDE; PECTINS; PLASMIDS; POLYSACCHARIDE-LYASES; RECOMBINATION, GENETIC; TEMPERATURE; TIME FACTORS;

BACILLUS SUBTILIS; ESCHERICHIA COLI;

EID: 80054843297     PISSN: 09608524     EISSN: 18732976     Source Type: Journal    
DOI: 10.1016/j.biortech.2011.09.020     Document Type: Article

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