Purification and immobilization of recombinant variants of Brevundimonas diminuta glutaryl-7-aminocephalosporanic acid acylase expressed in Escherichia coli cells

Journal of Biotechnology

Volumn 133, Issue 1, 2008, Pages 123-126

  Khatuntseva, S A ^a   Eldarov, M A ^a   Redo, V A ^a   Skryabin, K G ^a  

^a INSTITUTE OF BIOENGINEERING   (Russian Federation)

Author keywords

Affinity tag; Chitin binding domain; Fusion protein; Glutaryl 7 aminocephalosporanic acid acylase

Indexed keywords

ELUTION BUFFER; HOLOENZYMES; HYBRID PROTEINS; RECOMBINANT VARIANTS;

BIOCATALYSTS; CELL CULTURE; ENZYME ACTIVITY; ESCHERICHIA COLI; GENE EXPRESSION; MUTAGENESIS; PROTEINS;

AMINO ACIDS;

BACTERIAL ENZYME; CHITIN; CHITINASE; DNA; GLUTARYL 7 AMINOCEPHALOSPORANIC ACID ACYLASE; HYBRID PROTEIN; UNCLASSIFIED DRUG;

ALPHA CHAIN; ARTICLE; BIOCATALYST; BREVUNDIMONAS DIMINUTA; CONTROLLED STUDY; DNA MODIFICATION; ELUTION; ENZYME ACTIVITY; ENZYME BINDING; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN EXPRESSION;

AMIDOHYDROLASES; BACILLUS; ENZYME ACTIVATION; ENZYME STABILITY; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; PROTEIN ENGINEERING; RECOMBINANT PROTEINS; VARIATION (GENETICS);

BACILLUS CIRCULANS; BREVUNDIMONAS DIMINUTA; ESCHERICHIA COLI;

EID: 36049000963     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2007.08.044     Document Type: Article

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