A novel approach to quantify g-protein-coupled receptor dimerization equilibrium using bioluminescence resonance energy transfer

Methods in Molecular Biology

Volumn 1013, Issue , 2013, Pages 93-127

  Kufareva, Irina ^a   Stephens, Bryan ^a   Gilliland, C Taylor ^a   Wu, Beili ^b   Fenalti, Gustavo ^b   Hamel, Damon ^a   Stevens, Raymond C ^b   Abagyan, Ruben ^a   Handel, Tracy M ^a  

^a UNIVERSITY OF CALIFORNIA SAN DIEGO   (United States)

^b SCRIPPS RESEARCH INSTITUTE   (United States)

Author keywords

Bioluminescence resonance energy transfer; BRET saturation; BRET titration; Chemokine receptor; CXCR4; Dimerization; G protein coupled receptor; Monomer dimer equilibrium

Indexed keywords

CHEMOKINE RECEPTOR CXCR4; G PROTEIN COUPLED RECEPTOR; HETERODIMER; HOMODIMER; HYBRID PROTEIN;

ARTICLE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CELL ASSAY; CELL VIABILITY; CELLULAR DISTRIBUTION; CONCEPTUAL FRAMEWORK; CONTROLLED STUDY; DIMERIZATION; HUMAN; HUMAN CELL; MUTANT; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN PROTEIN INTERACTION; QUANTITATIVE ANALYSIS; WILD TYPE; ANIMAL; CELL CULTURE; CHEMICAL STRUCTURE; CHEMISTRY; GENETIC TRANSFECTION; GENETICS; METABOLISM; MUTATION; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION;

ANIMALS; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER TECHNIQUES; CELLS, CULTURED; HUMANS; MODELS, MOLECULAR; MUTATION; PROTEIN CONFORMATION; PROTEIN MULTIMERIZATION; RECEPTORS, CXCR4; RECEPTORS, G-PROTEIN-COUPLED; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

EID: 84886828758     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-426-5_7     Document Type: Article

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