Cell-Surface Protein–Protein Interaction Analysis with Time-Resolved FRET and Snap-Tag Technologies: Application to G Protein-Coupled Receptor Oligomerization

Methods in Molecular Biology

Volumn 756, Issue , 2011, Pages 201-214

  Comps Agrar, Laëtitia ^a   Maurel, Damien ^a   Rondard, Philippe ^a   Pin, Jean Philippe ^a   Trinquet, Eric ^b   Prézeau, Laurent ^a  

^a INSTITUT DE GÉNOMIQUE FONCTIONNELLE   (France)

^b Cisbio Bioassays   (France)

Author keywords

Dimerization; Fluorescence resonance energy transfer; G protein coupled receptor; GABAB receptor; SnapTag

Indexed keywords

4 AMINOBUTYRIC ACID B RECEPTOR; G PROTEIN COUPLED RECEPTOR;

ANIMAL; ARTICLE; CELL LINE; CHEMISTRY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMAN; METABOLISM; METHODOLOGY; PROTEIN ANALYSIS; PROTEIN MULTIMERIZATION;

ANIMALS; CELL LINE; FLUORESCENCE RESONANCE ENERGY TRANSFER; HUMANS; PROTEIN INTERACTION MAPPING; PROTEIN MULTIMERIZATION; RECEPTORS, G-PROTEIN-COUPLED; RECEPTORS, GABA-B;

EID: 80054769493     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-160-4_10     Document Type: Chapter

References (13)

1. Bouvier, M. (2001) Oligomerization of G-protein-coupled transmitter receptors. Nature Rev 2, 274–86.
2. Milligan, G. (2006) G-protein-coupled receptor heterodimers: pharmacology, function and relevance to drug discovery. Drug Discov Today 11, 541–9.
3. Pin, J. P., Neubig, R., Bouvier, M., Devi, L., Filizola, M., Javitch, J. A., Lohse, M. J., Milligan, G., Palczewski, K., Parmentier, M., and Spedding, M. (2007) International Union of Basic and Clinical Pharmacology. LXVII. Recommendations for the recognition and nomenclature of G protein-coupled receptor heteromultimers. Pharmacol Rev 59, 5–13.
4. Romano, C., Yang, W. L., and O’Malley, K. L. (1996) Metabotropic glutamate receptor 5 is a disulfide-linked dimer. J Biol Chem 271, 28612–6.
5. White, J. H., Wise, A., Main, M. J., Green, A., Fraser, N. J., Disney, G. H., Barnes, A. A., Emson, P., Foord, S. M., and Marshall, F. H. (1998) Heterodimerization is required for the formation of a functional GABA(B) receptor. Nature 396, 679–82.
6. 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET). Proc Natl Acad Sci U S A 97, 3684–9.
7. Overton, M. C., and Blumer, K. J. (2000) G-protein-coupled receptors function as oligomers in vivo. Curr Biol 10, 341–4.
8. Rocheville, M., Lange, D. C., Kumar, U., Patel, S. C., Patel, R. C., and Patel, Y. C. (2000) Receptors for dopamine and somatostatin: formation of hetero-oligomers with enhanced functional activity. Science 288, 154–7.
9. Bazin, H., Trinquet, E., and Mathis, G. (2002) Time resolved amplification of cryptate emission: a versatile technology to trace biomolecular interactions. Rev Mol Biotech 82, 233–50.
10. Mathis, G. (1995) Probing molecular interactions with homogeneous techniques based on rare earth cryptates and Xuorescence energy transfer. Clin Chem 41, 1391–7.
11. Maurel, D., Kniazeff, J., Mathis, G., Trinquet, E., Pin, J. P., and Ansanay, H. (2004) Cell surface detection of membrane protein interaction with homogeneous time-resolved fluorescence resonance energy transfer technology. Anal Biochem 329, 253–62.
12. Keppler, A., Gendreizig, S., Gronemeyer, T., Pick, H., Vogel, H., and Johnsson, K. A (2003) General method for the covalent labeling of fusion proteins with small molecules in vivo. Nat Biotechnol 21, 86–9.
13. Maurel, D., Comps-Agrar, L., Brock, C., Rives, M. L., Bourrier, E., Ayoub, M. A., Bazin, H., Tinel, N., Durroux, T., Prézeau, L., Trinquet, E., and Pin, J. P. (2008) Cell-surface protein–protein interaction analysis with time-resolved FRET and snap-tag technologies: application to GPCR oligomerization. Nat Methods 5, 561–7.