A rigorous experimental framework for detecting protein oligomerization using bioluminescence resonance energy transfer

Nature Methods

Volumn 3, Issue 12, 2006, Pages 1001-1006

  James, John R ^a   Oliveira, Marta I ^b,c   Carmo, Alexandre M ^b,c   Iaboni, Andrea ^a   Davis, Simon J ^a  

^a UNIVERSITY OF OXFORD   (United Kingdom)

^b UNIVERSITY OF PORTO   (Portugal)

^c UNIVERSITY OF PORTO   (Portugal)

Author keywords

[No Author keywords available]

Indexed keywords

4 AMINOBUTYRIC ACID; BETA 2 ADRENERGIC RECEPTOR; DIMER; G PROTEIN COUPLED RECEPTOR; LUCIFERASE; MEMBRANE PROTEIN; MONOMER; OLIGOMER;

ARTICLE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CELL MEMBRANE; CHIMERA; CONTROLLED STUDY; DIMERIZATION; ENERGY TRANSFER; EXPERIMENTAL DESIGN; HUMAN; HUMAN CELL; OLIGOMERIZATION; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION;

BINDING SITES; CELL MEMBRANE; DIMERIZATION; FLUORESCENCE RESONANCE ENERGY TRANSFER; LUMINESCENT MEASUREMENTS; LUMINESCENT PROTEINS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; RECEPTORS, G-PROTEIN-COUPLED;

EID: 33751215258     PISSN: 15487091     EISSN: 15491676     Source Type: Journal    
DOI: 10.1038/nmeth978     Document Type: Article

References (29)

1. Xu, Y., Piston, D.W. & Johnson, C.H. A bioluminescence resonance energy transfer (BRET) system: Application to interacting circadian clock proteins. Proc. Natl. Acad. Sci. USA 96, 151-156 (1999).
2. Wolber, P.K. & Hudson, B.S. An analytic solution to the Forster energy transfer problem in two dimensions. Biophys. J. 28, 197-210 (1979).
3. Fung, B.K. & Stryer, L. Surface density determination in membranes by fluorescence energy transfer. Biochemistry 17, 5241-5248 (1978).
4. Veatch, W. & Stryer, L. The dimeric nature of the gramicidin A transmembrane channel: Conductance and flourescence energy transfer studies of hybrid channels. J. Mol. Biol. 133, 89-102 (1977).
5. Kenworthy, A.K. & Edidin, M. Distribution of a glycosylphosphatidylinositol-anchored protein at the apical surface of MDCK cells examined at a resolution of <100 A using imaging flourescence resonance energy transfer. J. Cell Biol. 142, 69-84 (1998).
6. Pfleger, K.D. & Eidne, K.A. Monitoring the formation of dynamic G-protein-coupled receptor-protein complexes in living cells. Biochem. J. 385, 625-637 (2005).
7. Jones, E.Y., Davis, S.J., Williams, A.F., Harlos, K. & Stuart, D.I. Crystal structure at 2.8 A resolution of a soluble form of the cell adhesion molecule CD2. Nature 360, 232-239 (1992).
8. Bodian, D.L., Jones, E.Y., Harlos, K., Stuart, D.I. & Davis, S.J. Crystal structure of the extracellular region of the human cell adhesion molecule CD2 at 2.5 A resolution. Structure 2, 755-766 (1994).
9. Collins, A.V. et al. The interaction properties of costimulatory molecules revisited. Immunity 17, 201-210 (2002).
10. Ikemizu, S. et al. Structure and dimerization of a soluble form of B7-1. Immunity 12, 51-60 (2000).
11. Stamper, C.C. et al. Crystal structure of the B7-1/CTLA-4 complex that inhibits human immune responses. Nature 410, 608-611 (2001).
12. Barclay, A.N. et al. The Leucocyte Antigen Factsbook (Academic Press, San Diego, 1997).
13. Shaner, N.C. et al. Improved monomeric red, orange and yellow fluorescent proteins derived from Discosoma sp. red fluorescent protein. Nat. Biotechnol. 22, 1567-1572 (2004).
14. Mercier, J.F., Salahpour, A., Angers, S., Breit, A. & Bouvier, M. Quantitative assessment of beta 1- and beta 2-adrenergic receptor homo- and heterodimerization by bioluminescence resonance energy transfer. J. Biol. Chem. 277, 44925-44931 (2002).
15. Bhatia, S., Edidin, M., Almo, S.C. & Nathenson, S.G. Different cell surface oligomeric states of B7-1 and B7-2: Implications for signaling. Proc. Natl. Acad. Sci. USA 102, 15569-15574 (2005).
16. Angers, S. et al. Detection of beta 2-adrenergic receptor dimerization in living cells using bioluminescence resonance energy transfer (BRET). Proc. Natl. Acad. Sci. USA 97, 3684-3689 (2000).
17. Ramsay, D., Kellett, E., McVey, M., Rees, S. & Milligan, G. Homo- and hetero-oligomeric interactions between G-protein-coupled receptors in living cells monitored by two variants of bioluminescence resonance energy transfer (BRET): Hetero-oligomers between receptors subtypes form more efficiently than between less closely related sequences. Biochem. J. 365, 429-440 (2002).
18. Chen, Y., Couvinea, A., Laburthe, M. & Amiranoff, B. Solubilization and molecular characterization of active galamin receptors from rat brain. Biochemistry 31, 2415-2422 (1992).
19. White, J.H. et al. Heterodimerization is required for the formation of a functional GABA(B) receptor. Nature 396, 679-682 (1998).
20. Jones, K.A. et al. GABA(B) receptors function as a heteromeric assembly of the subunits GABA(B)R1 and GABA(B)R2. Nature 396, 674-679 (1998).
21. Canals, M. et al. Homodimerization of adenosine A2A receptors: qualitative and quantitative assessment by fluorescence and bioluminescence energy transfer. J. Neurochem. 88, 726-734 (2004).
22. Canals, M. et al. Adenosine A2A-dopamine D2 receptor-receptor heteromerization: Qualitative and quantitative assessment by fluorescence and bioluminescence energy transfer. J. Biol. Chem. 278, 46741-46749 (2003).
23. Percherancier, Y. et al. Bioluminescence resonance energy transfer reveals ligand-induced conformational changes in CXCR4 homo- and heterodimers. J. Biol. Chem. 280, 9895-9903 (2005).
24. Breit, A., Lagace, M. Bouvier, M. Hetero-oligomerization between beta2-and beta3-adrenergic receptors generates a beta-adrenergic signaling unit with distinct functional properties. J. Biol. Chem. 279, 28756-28765 (2004).
25. Uezono, Y. et al. Coupling of GABAB receptor GABAB2 subunit to G proteins: Evidence from Xenopus oocyte and baby hamster kidney cell expression system. Am. J. Physiol. Cell Physiol. 290, C200-C207 (2006).
26. Saxton, M.J. Lateral diffusion in an archipelago. Distance dependence of the diffusion coefficient. Biophys. J. 56, 615-622 (1989).
27. Jun, J.E. & Goodnow, C.C. Scaffolding of antigen receptors for immunogenic versus tolerogenic signaling. Nat. Immunol. 4, 1057-1064 (2003).
28. Davis, S.J. & van der Merwe, P.A. The structure and ligand interactions of CD2: Implications for T-cell function. Immunol. Today 17, 177-187 (1996).
29. Davis, D.M. & Dustin, M.L. What is the importance of the immunological synapse? Trends Immunol. 25, 323-327 (2004).