Identification and characterization of an alternative splice variant of Mpl with a high affinity for TPO and its activation of ERK1/2 signaling

International Journal of Biochemistry and Cell Biology

Volumn 45, Issue 12, 2013, Pages 2852-2863

  Wang, Qiong ^a   Sun, Rui ^b   Wu, Leyan ^a   Huang, Junfeng ^a   Wang, Ping ^a   Yuan, Hailong ^a   Qiu, Feifei ^a   Xu, Xiaohong ^c   Wu, Di ^a   Yu, Ying ^a   Liu, Xin ^a   Zhang, Qing ^a  

^a SUN YAT SEN UNIVERSITY   (China)

^b SUN YAT SEN UNIVERSITY CANCER CENTER   (China)

^c GUANGZHOU UNIVERSITY OF CHINESE MEDICINE   (China)

Author keywords

Active conformation; Binding affinity; ERK1 2 signals; Mpl splice variant; mRNA expression levels

Indexed keywords

CYCLIN D1; CYCLIN D2; MITOGEN ACTIVATED PROTEIN KINASE 1; MITOGEN ACTIVATED PROTEIN KINASE 3; MITOGEN ACTIVATED PROTEIN KINASE P38; PROTEIN BAX; PROTEIN BCL XL; PROTEIN MPL; THROMBOPOIETIN; THROMBOPOIETIN RECEPTOR; UNCLASSIFIED DRUG;

ALTERNATIVE RNA SPLICING; ARTICLE; BINDING AFFINITY; CELL CYCLE G1 PHASE; CELL CYCLE G2 PHASE; CELL CYCLE S PHASE; CELL DIFFERENTIATION; CELL PROLIFERATION; CELL SURVIVAL; CELLULAR DISTRIBUTION; CONTROLLED STUDY; DOWN REGULATION; G1 PHASE CELL CYCLE CHECKPOINT; HUMAN; HUMAN CELL; PROTEIN CONFORMATION; PROTEIN EXPRESSION; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION;

ACTIVATION DOMAIN; ACTIVE CONFORMATION; AD; BINDING AFFINITY; CDK; CRM; CYCLIN-DEPENDENT KINASES; CYTOKINE RECEPTOR MODULE; DNA BINDING DOMAIN; DNA-BD; EBP; EPO-BINDING PROTEIN; ERK1/2; ERK1/2 SIGNALS; EXTRACELLULAR SIGNAL-REGULATED PROTEIN KINASES-1 AND -2; ICD; INTRACELLULAR DOMAIN; MPL; MPL SPLICE VARIANT; MPL-EC; MRNA EXPRESSION LEVELS; P-ERK1/2; P-P38; P38 MAPK; P38 MITOGEN-ACTIVATED PROTEIN KINASE; PHOSPHORYLATED ERK1/2; PHOSPHORYLATED P38; REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION; RT-PCR; S-PHAGE PHASE FRACTION; SIGNAL PEPTIDE; SP; SPF; T-ERK1/2; T-P38; THE EXTRACELLULAR DOMAIN OF THROMBOPOIETIN RECEPTOR; THE THROMBOPOIETIN RECEPTOR; THROMBOPOIETIN; TM; TOTAL ERK1/2; TOTAL-P38; TPO; TRANSMEMBRANE DOMAIN;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; BASE SEQUENCE; CELL DIFFERENTIATION; CELL GROWTH PROCESSES; CELL LINE, TUMOR; HUMANS; MAP KINASE SIGNALING SYSTEM; MICE; MOLECULAR SEQUENCE DATA; NIH 3T3 CELLS; PROTEIN CONFORMATION; RECEPTORS, THROMBOPOIETIN; SIGNAL TRANSDUCTION; THROMBOPOIETIN;

EID: 84886735653     PISSN: 13572725     EISSN: 18785875     Source Type: Journal    
DOI: 10.1016/j.biocel.2013.09.010     Document Type: Article

References (47)

1. W.S. Alexander, and A.R. Dunn Structure and transcription of the genomic locus encoding murine c-Mpl, a receptor for thrombopoietin Oncogene 10 1995 795 803
2. W.S. Alexander, D. Metcalf, and A.R. Dunn Point mutations within a dimer interface homology domain of c-Mpl induce constitutive receptor activity and tumorigenicity EMBO J 14 1995 5569 5578
3. K. Arnold, L. Bordoli, J. Kopp, and T. Schwede The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling Bioinformatics (Oxford, England) 22 2006 195 201 (Pubitemid 43205406)
4. M. Ballmaier c-Mpl mutations are the cause of congenital amegakaryocytic thrombocytopenia Blood 97 2001 139 146 (Pubitemid 32061253)
5. R.D. Baynes, G.K. Reddy, Y.J. Shih, B.S. Skikne, and J.D. Cook Serum form of the erythropoietin receptor identified by a sequence-specific peptide antibody Blood 82 1993 2088 2095 (Pubitemid 23293991)
6. R. Besancenot, R. Chaligne, C. Tonetti, F. Pasquier, C. Marty, and Y. Lecluse A senescence-like cell-cycle arrest occurs during megakaryocytic maturation: implications for physiological and pathological megakaryocytic proliferation PLoS Biol 2010 2010
7. V.C. Broudy, N.L. Lin, D.F. Sabath, T. Papayannopoulou, and K. Kaushansky Human platelets display high-affinity receptors for thrombopoietin Blood 89 1997 1896 1904 (Pubitemid 27132103)
8. W.M. Chen, B. Yu, Q. Zhang, and P. Xu Identification of the residues in the extracellular domain of thrombopoietin receptor involved in the binding of thrombopoietin and a nuclear distribution protein (human NUDC) J Biol Chem 285 2010 26697 26709
9. S. Chiba, T. Takahashi, K. Takeshita, J. Minowada, Y. Yazaki, and F.H. Ruddle Selective expression of mRNA coding for the truncated form of erythropoietin receptor in hematopoietic cells and its decrease in patients with polycythemia vera Blood 90 1997 97 104 (Pubitemid 27276552)
10. J. Coers, C. Ranft, and R.C. Skoda A truncated isoform of c-Mpl with an essential C-terminal peptide targets the full-length receptor for degradation J Biol Chem 279 2004 36397 36404 (Pubitemid 39128978)
11. F.J. de Sauvage, P.E. Hass, S.D. Spencer, B.E. Malloy, A.L. Gurney, and S.A. Spencer Stimulation of megakaryocytopoiesis and thrombopoiesis by the c-Mpl ligand Nature 369 1994 533 538
12. C.M. Deane, R.T. Kroemer, and W.G. Richards A structural model of the human thrombopoietin receptor complex J Mol Graphics Modell 15 1997 85 88 170-8
13. W.F. DeGrado, H. Gratkowski, and J.D. Lear How do helix-helix interactions help determine the folds of membrane proteins? Perspectives from the study of homo-oligomeric helical bundles Protein Sci 12 2003 647 665 (Pubitemid 36348451)
14. J. Ding, H. Komatsu, A. Wakita, M. Kato-Uranishi, M. Ito, and A. Satoh Familial essential thrombocythemia associated with a dominant-positive activating mutation of the c-MPL gene, which encodes for the receptor for thrombopoietin Blood 103 2004 4198 4200 (Pubitemid 38685363)
15. M.D. Feese, T. Tamada, Y. Kato, Y. Maeda, M. Hirose, and Y. Matsukura Structure of the receptor-binding domain of human thrombopoietin determined by complexation with a neutralizing antibody fragment Proc Natl Acad Sci U S A 101 2004 1816 1821 (Pubitemid 38228974)
16. J. Han, Y. Tsukada, E. Hara, N. Kitamura, and T. Tanaka Hepatocyte growth factor induces redistribution of p21CIP1 and p27KIP1 through ERK-dependent p16INK4a up-regulation, leading to cell cycle arrest at G1 in HepG2 hepatoma cells J Biol Chem 280 2005 31548 31556 (Pubitemid 41291898)
17. K. Kaushansky, S. Lok, R.D. Holly, V.C. Broudy, N. Lin, and M.C. Bailey Promotion of megakaryocyte progenitor expansion and differentiation by the c-Mpl ligand thrombopoietin Nature 369 1994 568 571 (Pubitemid 24192526)
18. J.J. Kiladjian, N. Elkassar, G. Hetet, J. Briere, B. Grandchamp, and C. Gardin Study of the thrombopoitin receptor in essential thrombocythemia Leukemia 11 1997 1821 1826 (Pubitemid 27485125)
19. M.J. Koury, M.C. Bondurant, and S.S. Rana Changes in erythroid membrane proteins during erythropoietin-mediated terminal differentiation J Cell Physiol 133 1987 438 448 (Pubitemid 18014254)
20. J. Li, D.F. Sabath, and D.J. Kuter Cloning and functional characterization of a novel c-mpl variant expressed in human CD34 cells and platelets Cytokine 12 2000 835 844 (Pubitemid 30479464)
21. J. Li, Y. Xia, and D.J. Kuter Interaction of thrombopoietin with the platelet c-Mpl receptor in plasma: binding, internalization, stability and pharmacokinetics Br J Haematol 106 1999 345 356 (Pubitemid 29450656)
22. O. Livnah Crystallographic evidence for preformed dimers of erythropoietin receptor before ligand activation Science 283 1999 987 990
23. S.M. Luoh, E. Stefanich, G. Solar, H. Steinmetz, T. Lipari, and T.I. Pestina Role of the distal half of the c-Mpl intracellular domain in control of platelet production by thrombopoietin in vivo Mol Cell Biol 20 2000 507 515 (Pubitemid 30023092)
24. S. Malinge, C. Ragu, V. Della-Valle, D. Pisani, S.N. Constantinescu, and C. Perez Activating mutations in human acute megakaryoblastic leukemia Blood 112 2008 4220 4226
25. S. Meloche, and J. Pouyssegur The ERK1/2 mitogen-activated protein kinase pathway as a master regulator of the G1-to S-phase transition Oncogene 26 2007 3227 3239 (Pubitemid 46763017)
26. G.A. Millot, W. Vainchenker, D. Dumenil, and F. Svinarchuk Distinct effects of thrombopoietin depending on a threshold level of activated Mpl in BaF-3 cells J Cell Sci 115 2002 2329 2337 (Pubitemid 34752976)
27. Y. Nakamura, N. Komatsu, and H. Nakauchi A truncated erythropoietin receptor that fails to prevent programmed cell death of erythroid cells Science 257 1992 1138 1141
28. T.D. Osslund Structural basis for the signal transduction of erythropoietin Erythropoietins and erythropoiesis 2009 143 153
29. N. Papadantonakis, M. Makitalo, D. McCrann, K. Liu, H. Nguyen, and G. Martin Direct visualization of the endomitotic cell cycle in living megakaryocytes: differential patterns in low and high ploidy cells Cell Cycle 7 2008 2352 2356
30. C. Perez, J. Vandesompele, I. Vandenbroucke, G. Holtappels, F. Speleman, and P. Gevaert Quantitative real time polymerase chain reaction for measurement of human interleukin-5 receptor alpha spliced isoforms mRNA BMC Biotechnol 3 2003 17
31. E.F. Pettersen, T.D. Goddard, C.C. Huang, G.S. Couch, D.M. Greenblatt, and E.C. Meng UCSF chimera - a visualization system for exploratory research and analysis J Comput Chem 25 2004 1605 1612
32. B.G. Pierce, Y. Hourai, and Z. Weng Accelerating protein docking in ZDOCK using an advanced 3D convolution library PLoS ONE 6 2011 e24657
33. Y. Pikman, B.H. Lee, T. Mercher, E. McDowell, B.L. Ebert, and M. Gozo MPLW515L is a novel somatic activating mutation in myelofibrosis with myeloid metaplasia PLoS Med 3 2006 e270
34. S. Ramachandran, P. Kota, F. Ding, and N.V. Dokholyan Automated minimization of steric clashes in protein structures Proteins 79 2011 261 270
35. P. Rojnuckarin, J.G. Drachman, and K. Kaushansky Thrombopoietin-induced activation of the mitogen-activated protein kinase (MAPK) pathway in normal megakaryocytes: role in endomitosis Blood 94 1999 1273 1282 (Pubitemid 29380408)
36. K. Roovers, G. Davey, X. Zhu, M.E. Bottazzi, and R.K. Assoian Alpha5beta1 integrin controls cyclin D1 expression by sustaining mitogen-activated protein kinase activity in growth factor-treated cells Mol Biol Cell 10 1999 3197 3204 (Pubitemid 29489555)
37. M.C. Rouyez, C. Boucheron, S. Gisselbrecht, I. Dusanter-Fourt, and F. Porteu Control of thrombopoietin-induced megakaryocytic differentiation by the mitogen-activated protein kinase pathway Mol Cell Biol 17 1997 4991 5000 (Pubitemid 27357598)
38. D.F. Sabath, K. Kaushansky, and V.C. Broudy Deletion of the extracellular membrane-distal cytokine receptor homology module of Mpl results in constitutive cell growth and loss of thrombopoietin binding Blood 94 1999 365 367 (Pubitemid 29300171)
39. F. Sayani, F.A. Montero-Julian, V. Ranchin, J.M. Prevost, S. Flavetta, and W. Zhu Identification of the soluble granulocyte-macrophage colony stimulating factor receptor protein in vivo Blood 95 2000 461 469 (Pubitemid 30041657)
40. N.J. Slater, M. Yamaguchi, D.G. Rothwell, P. Baker, C.M. Heyworth, and R. Chopra The human granulocyte/macrophage colony-stimulating factor receptor alpha2 isoform influences haemopoietic lineage commitment and divergence Br J Haematol 122 2003 150 158 (Pubitemid 36819350)
41. J. Tavernier, J. Van der Heyden, A. Verhee, G. Brusselle, X. Van Ostade, and J. Vandekerckhove Interleukin 5 regulates the isoform expression of its own receptor alpha-subunit Blood 95 2000 1600 1607 (Pubitemid 30120819)
42. D.E. Todd, R.M. Densham, S.A. Molton, K. Balmanno, C. Newson, and C.R. Weston ERK1/2 and p38 cooperate to induce a p21CIP1-dependent G1 cell cycle arrest Oncogene 23 2004 3284 3295 (Pubitemid 38669818)
43. W. Vainchenker, N. Methia, N. Debili, M. Titeux, and F. Wendling c-Mpl, the thrombopoietin receptor Thromb Haemost 74 1995 526 528
44. I. Vigon, J.P. Mornon, L. Cocault, M.T. Mitjavila, P. Tambourin, and S. Gisselbrecht Molecular cloning and characterization of MPL, the human homolog of the v-mpl oncogene: identification of a member of the hematopoietic growth factor receptor superfamily Proc Natl Acad Sci U S A 89 1992 5640 5644
45. H. Yawata, K. Yasukawa, S. Natsuka, M. Murakami, K. Yamasaki, and M. Hibi Structure-function analysis of human IL-6 receptor: dissociation of amino acid residues required for IL-6-binding and for IL-6 signal transduction through gp130 EMBO J 12 1993 1705 1712 (Pubitemid 23112825)
46. Y.P. Zhang, Y.S. Tang, X.S. Chen, and P. Xu Regulation of cell differentiation by hNUDC via a Mpl-dependent mechanism in NIH 3T3 cells Exp Cell Res 313 2007 3210 3221 (Pubitemid 47284056)
47. J. Zimmet, and K. Ravid Polyploidy: occurrence in nature, mechanisms, and significance for the megakaryocyte-platelet system Exp Hematol 28 2000 3 16 (Pubitemid 30047186)