Direct visualization of the endomitotic cell cycle in living megakaryocytes: Differential patterns in low and high ploidy cells

Cell Cycle

Volumn 7, Issue 15, 2008, Pages 2352-2356

  Papadantonakis, Nicholas ^a   Makitalo, Maria ^a   McCrann, Donald J ^a   Liu, Kenian ^a   Nguyen, Hao G ^a   Martin, Greg ^a   Patel Hett, Sunita ^b,c   Italiano, Joseph E ^b,c   Ravid, Katya ^a  

^a BOSTON UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b BRIGHAM AND WOMEN S HOSPITAL   (United States)

^c BOSTON CHILDREN S HOSPITAL   (United States)

Author keywords

Cell cycle; Endomitosis; Hematopoiesis; Megakaryocytes; Polyploidy

Indexed keywords

GREEN FLUORESCENT PROTEIN; HISTONE H2B;

ANAPHASE; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ARTICLE; CELL CYCLE; CHROMOSOME CONDENSATION; CHROMOSOME SEGREGATION; CYTOKINESIS; DNA REPLICATION; ENDOMITOSIS; GENETIC ENGINEERING; MEGAKARYOCYTE; MITOSIS; MOUSE; NONHUMAN; PLOIDY; PROTEIN EXPRESSION; REGULATORY MECHANISM; TRANSGENIC MOUSE;

MUS MUSCULUS;

EID: 48849098702     PISSN: 15384101     EISSN: 15514005     Source Type: Journal    
DOI: 10.4161/cc.6325     Document Type: Article

References (21)

1. Ravid K, Lu J, Zimmet JM, Jones MR. Roads to polyploidy: the megakaryocyte example. J Cell Physiol 2002; 190:7-20.
2. Nagata Y, Yoshinao M, Todokoro K. Thrombopoietin-induced polyploidization of bone marrow megakaryocytes is due to a unique regulatory mechanism in late mitosis. The Journal of Cell Biology 1997; 139:449-57.
3. Zimmet J, Ravid K. Polyploidy: Occurrence in nature, mechanisms, and significance for the megakaryocyte-platelet system. Experimental Hematology 2000; 28:3-16.
4. Straight AF, Field CM. Microtubules, membranes and cytokinesis. Curr Biol 2000; 10:760-70.
5. Schuyler SC, Liu JY, Pellman D. The molecular function of Ase1p: evidence for a MAP-dependent midzone-specific spindle matrix. J Cell Biol 2003; 160:517-28.
6. Vitrat N, Cohen-Solal K, Pique C, LeCouedic JP, Norol F, Larsen AK, Katz A, Vainchenker W, Debili N. Endomitosis of Human Megakaryocytes Are Due to Abortive Mitosis. Blood 1998; 91:3711-23.
7. Roy L, Coullin P, Vitrat N, Hellio R, Debili N, Weinstein J, Bernheim A, Vainchenker W. Asymmetrical segregation of chromosomes with a normal metaphase/anaphase checkpoint in polyploid megakaryocytes. Blood 2001; 97:2238-47.
8. Nagata Y, Muro Y, Todokoro K. Thrombopoietin-induced Polyploidization of Bone Marrow Megakaryocytes Is Due to a Unique Regulatory Mechanism in Late Mitosis. J Cell Biol 1997; 139:449-57.
9. Geddis AE, Fox NE, Tkachenko E, Kaushansky K. Endomitotic megakaryocytes that form a bipolar spindle exhibit cleavage furrow ingression followed by furrow regression. Cell Cycle 2007; 6:455-60.
10. Ravid K, Beeler DL, Rabin MS, Ruley HE, Rosenberg RD. Selective Targeting of Gene Products with the Megakaryocyte Platelet Factor 4 Promoter. PNAS 1991; 88:1521-5.
11. Zhang Y, Nagata Y, Yu G, Nguyen HG, Jones MR, Toselli P, Jackson CW, Tatsuka M, Todokoro K, Ravid K. Aberrant quantity and localization of Aurora-B/AIM-1 and survivin during megakaryocyte polyploidization and the consequences of Aurora-B/AIM-1-deregulated expression. Blood 2004; 103:3717-26.
12. Zimmet J, Toselli P, Ravid K. Cyclin D3 and megakaryocyte development: exploration of a transgenic phenotype. Stem Cells 1998; 16:97-106.
13. Levine RF, Hazzard KC, Lamberg JD. The significance of megakaryocyte size. Blood 1982; 60:1122-31.
14. Tomer A. Human marrow megakaryocyte differentiation: multiparameter correlative analysis identifies von Willebrand factor as a sensitive and distinctive marker for early (2N and 4N) megakaryocytes. Blood 2004; 104:2722-7.
15. Italiano JE Jr, Lecine P, Shivdasani RA, Hartwig JH. Blood Platelets Are Assembled Principally at the Ends of Proplatelet Processes Produced by Differentiated Megakaryocytes. J Cell Biol 1999; 147:1299-312.
16. Patel SR, Richardson JL, Schulze H, Kahle E, Galjart N, Drabek K, Shivdasani RA, Hartwig JH, Italiano JE Jr. Differential roles of microtubule assembly and sliding in proplatelet formation by megakaryocytes. Blood 2005; 106:4076-85.
17. Marino-Ramirez L, Kann MG, Shoemaker BA, Landsman D. Histone structure and nucleosome stability. Expert Review of Proteomics 2005; 2:719-29.
18. Kanda TSK, Wahl GM. Histone-GFP fusion protein enables sensitive analysis of chromosome dynamics in living mammalian cells. Curr Biol 1998; 8:377-85.
19. Yagi M, Roth GJ. Megakaryocyte polyploidization is associated with decreased expression of polo-like kinase (PLK). J Thromb Haemost 2006; 4:2028-34.
20. Zimmet JM, Ladd D, Jackson CW, Stenberg PE, Ravid K. A role for cyclin D3 in the endomitotic cell cycle. Molecular and Cellular Biology 1997; 17:7248-59.
21. Geng Y, Yu Q, Sicinska E, Das M, Schneider JE, Bhattacharya S, Rideout WM, Bronson RT, Gardner H, Sicinski P. Cyclin E Ablation in the Mouse. Cell 2003; 114:431-43.