Quantitative real time polymerase chain reaction for measurement of human Interleukin-5 receptor alpha spliced isoforms mRNA

BMC Biotechnology

Volumn 3, Issue , 2003, Pages

  Pérez, Claudina ^a   Vandesompele, Jo ^a   Vandenbroucke, Ina ^a   Holtappels, Gabriele ^a   Speleman, Frank ^a   Gevaert, Philippe ^a   Van Cauwenberge, Paul ^a   Bachert, Claus ^a  

^a GHENT UNIVERSITY HOSPITAL   (Belgium)

Author keywords

Alternative splicing; Eosinophilic chronic rhinosinusitis; Membrane anchored interleukin 5 receptor alpha; Peripheral blood; Real time PCR; Soluble interleukin 5 receptor alpha; SYBR Green I

Indexed keywords

COMPLEMENTARY DNA; FLUORESCENT DYE; INTERLEUKIN 5 RECEPTOR; INTERLEUKIN 5 RECEPTOR ALPHA; ISOPROTEIN; MESSENGER RNA; PRIMER RNA; UNCLASSIFIED DRUG; IL5RA PROTEIN, HUMAN; INTERLEUKIN RECEPTOR; ORGANIC COMPOUND; SYBR GREEN I;

ACCURACY; ANALYTIC METHOD; ARTICLE; BLOOD; CHRONIC DISEASE; CHRONIC INFLAMMATION; CONTROLLED STUDY; EOSINOPHIL; GENE CONSTRUCT; GENE OVEREXPRESSION; GENETIC CODE; GENETIC VARIABILITY; HUMAN; HUMAN CELL; HUMAN TISSUE; MEMBRANE BINDING; MOLECULAR DYNAMICS; NUCLEOTIDE SEQUENCE; PATHOGENESIS; PROTEIN EXPRESSION; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REGULATORY MECHANISM; RELIABILITY; RHINOSINUSITIS; RNA SPLICING; SENSITIVITY ANALYSIS; STANDARD; ALTERNATIVE RNA SPLICING; EOSINOPHILIA; EVALUATION; GENETICS; IMMUNOLOGY; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; POLYMERASE CHAIN REACTION; REPRODUCIBILITY; SINUSITIS; TIME;

ALTERNATIVE SPLICING; EOSINOPHILIA; FLUORESCENT DYES; HUMANS; INTERLEUKIN-5 RECEPTOR ALPHA SUBUNIT; MOLECULAR SEQUENCE DATA; ORGANIC CHEMICALS; POLYMERASE CHAIN REACTION; PROTEIN ISOFORMS; RECEPTORS, INTERLEUKIN; RECEPTORS, INTERLEUKIN-5; REPRODUCIBILITY OF RESULTS; RNA, MESSENGER; SINUSITIS; TIME FACTORS;

EID: 3042613336     PISSN: 14726750     EISSN: None     Source Type: Journal    
DOI: 10.1186/1472-6750-3-17     Document Type: Article

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