Bioactive Proteins and Peptides from Egg Proteins

Bioactive Proteins and Peptides as Functional Foods and Nutraceuticals

Volumn , Issue , 2010, Pages 247-263

  Wu, Jianping ^a   Majumder, Kaustav ^a   Gibbons, Kristen ^a  

^a UNIVERSITY OF ALBERTA   (Canada)

Author keywords

Anticancer proteins and peptides; Antihypertensive peptides; Antimicrobial peptides from lysozyme; Antimicrobial peptides from ovalbumin; Antimicrobial proteins and peptides from eggs; Antioxidant proteins and peptides; Bioactive proteins and peptides from egg proteins; Immunomodulating proteins and peptides; Ovotransferrin; Protease inhibitors

Indexed keywords

EID: 84886469592     PISSN: None     EISSN: None     Source Type: Book    
DOI: 10.1002/9780813811048.ch17     Document Type: Chapter

References (155)

1. Abdou AM, Higashiguchi S, Aboueleinin AM, Kim M, Ibrahim HR. 2007. Antimicrobial peptides derived from hen egg lysozyme with inhibitory effect against Bacillus species. Food Control 18 (2): 137-178.
2. Abdullah FB, Chahine JM. 1999. Transferrins, the mechanism of iron release by ovotransferrin. Eur J Biochem 263 (3): 912-920.
3. Albright KJ, Gordon DT, Cotterill OJ. 1984. Release of iron from phosvitin by heat and food additives. J Food Sci 49 (1): 78-81.
4. Ames BN, Shigenaga MK, Hagen TM. 1993. Oxidants, antioxidants, and the degenerative diseases of aging. PNAS 90 (17): 7915-7922.
5. Araki S, Suzuki M, Fijimoto M. 1992. Enhanced resistance to bacterial infections in mice by oral administration of an active egg white product. J Vet Med 54 (5): 1055-1056.
6. Asakura K, Kojima T, Shirasaki H, Kataura A. 1990. Evaluation of the effects of antigen specific immunotherapy on chronic sinusitis in children with allergy. Auris Nasus Larynx 17 (1): 33-38.
7. Atkinson AB, Robertson JIS. 1979. Captopril in the treatment of clinical hypertension and cardiac failure. Lancet 2 (8147): 836-839.
8. Bangalore A, Travis J, Onunka VC, Pohl J, Shafer WM. 1990. Identification of the primary antimicrobial domains in human neutrophil cathepsin G. J Biol Chem 265 (23): 13584-13588.
9. Baron F, Cochet MF, Pellerin JL, Ben Zakour N, Lebon A, Navarro A, Proudy I, Le Loir Y, Gautier M. 2004. Development of a PCR test for the differentiation between Staphylococcus aureus and Staphylococcus intermedius. J Food Prot 67 (10): 2302-2305.
10. Baron F, Rehault S. 2007. Compounds with antimicrobial activity . In: R Huopalahti, R López-Fandiño, M Anton, R Schade, eds., Bioactive Egg Compounds, 191-198. Berlin: Springer. Bjorck L. 1990. Proteinase inhibition, immunoglobulin-binding proteins and a novel antimicrobial principle. Mol Microbiol 4 (9): 1439-1442.
11. Bolder NM. 1997. Decontamination of meat and poultry carcasses . Trends Food Sci Technol 8 (7): 221-227.
12. Bullen JJ, Rogers HJ, Griffiths E. 1978. Role of iron in bacterial infection. Curr Top Microbiol Immunol 80 (1): 1-35.
13. CCS (Canadian Cancer Society). 2007. http://cancer.ca/ccs/internet/ standard/0,3182,3172_14279_371283_langIden,00.html, accessed on June 4, 2007.
14. Charter EA, Lagarde G. 2004. Natural antimicrobial systems/ lysozyme and other proteins in eggs. In: RK Robinson, ed., Encyclopedia of Food Microbiology, Vols. 1-3, 1582-1587. San Diego: Academic Press Elsevier.
15. Choi I, Jung C, Seog H, Choi H. 2004. Purification of phosvitin from egg yolk and determination of its physiochemical properties . Food Sci Biotech 13 (4): 434-437.
16. Colella R, Sakaguchi Y, Nagase H, Bird JW. 1989. Chicken egg white lysozyme. Molecular cloning, nucleotide sequence, and tissue distribution. J Biol Chem 264 (29): 17164-17169.
17. Cotterill OJ, Geiger GS. 1977. Egg product yield trends from shell eggs. Poult Sci 56 (3): 1027-1031.
18. Coussens LM, Fingleton B, Matrisian LM. 2002. Matrix metalloproteinase inhibitors and cancer: Trials and tribulations. Science 295 (5564): 2387-2392.
19. Cunningham EE, Procter VA, Goetsch SJ. 1991. Egg-white lysozyme as a food preservative: An overview. World Poult Sci 47 (2): 141-163.
20. Daeschel MA, Bruslind L, Clawson J. 1999. Application of the enzyme lysozyme in brewing. MBAA TQ 36 (2): 219-222.
21. Damiens E, Yazidi EI, Mazurier J, Duthille I, Spik G, Boilly-Marer Y. 1999. Lactoferrin inhibits G1 cyclin-dependent kinases during growth arrest of human breast carcinoma cells. J Cell Biochem 74 (3): 486-498.
22. Das L, Datta N, Bandyopadhyay S, Das PK. 2001. Successful therapy of lethal murine visceral leishmaniasis with cystatin involves up-regulation of nitric oxide and a favourable T cell response. J Immunol 166 (6): 4020-4028.
23. Davalos A, Miguel M, Bartolome B, López-Fandiño R. 2004. Antioxidant activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Prot 67 (9): 1939-1944.
24. Durance TD, Nakai S. 1988. Purification of avidin by cation exchange, gel filtration, metal chelate interaction and hydrophobic interaction chromatography. Can Inst Food Sci Technol J 21 (3): 279-286.
25. Düring K, Porsch P, Mahn A, Brinkmann O, Gieffers W. 1999. The non-enzymatic microbicidal activity of lysozymes. FEMS Lett 449 (2-3): 93-100.
26. Eaton SB, Konner M. 1985. Paleolithic nutrition. A consideration of its nature and current implications. N Engl J Med 312 (5): 283-289.
27. Ebina T, Tsukada K. 1991. Protease inhibitors prevent the development of human rotavirus-induced diarrhea in suckling mice. Microbiol Immunol 35 (7): 583-588.
28. Feinman RD, Fine EJ. 2003. Thermodynamics and metabolic advantage of weight loss diets. Metabol Synd Related Disorders 1 (3): 209-219.
29. FitzGerald RJ, Murray BA, Walsh DJ. 2004. Hypotensive peptides from milk proteins. J Nutr 134 (4): 980-988.
30. Fossum K, Whitaker JR. 1968. Ficin and papain inhibitor chicken egg white. Arch Biochem Biophys 125 (1): 367-375.
31. Fujita H, Usui H, Kurahashi K, Yoshikawa M. 1995. Isolation and characterization of Ovokinin, a bradykinin B1 agonist peptide derived from ovalbumin. Peptides 16 (5): 785-790.
32. Galzie Z, Qasim MA, Salahuddin A. 1996. Isolation and characterization of domain I of ovoinhibitor. Biochem Biophys Acta 1293 (1): 113-121.
33. Gasparri A, Moro M, Curnis F, Sacchi A, Pagano S, Veglia F, Casorati G, Siccardi AG, Dellabona P, Corti A. 1999. Tumour pretargeting with Avidin improves the therapeutic index of biotinylated tumour necrosis factor α in mouse model. Cancer Res 59 (12): 2917-2923.
34. Gautron J, Hincke MT, Mann K, McKee MD, Bain M, Solomon SE, Nys Y. 2003. Isolation, amino acid sequencing, cloning, localization and expression of ovocalyxin-32, a novel chicken eggshell matrix protein. In: I Kobayashi, H Ozawa, eds., Proceedings of the 8th International Symposium on Biomineralization, 163-166. Kanagawa: Tokai University Press.
35. Gautron J, Hincke MT, Panhéleux M, Garcia-Ruiz JM, Boldicke T, Nys Y. 2001. Ovotransferrin is a matrix protein of the hen eggshell membranes and basal calcified layer. Connect Tissue Res 42 (4): 255-267.
36. Gautron J, Murayama E, Vignal A, Morisson M, McKee MD, Rehault S, Labas V, Belghazi M, Vidal ML, Nys Y, Hincke MT. 2007. Cloning of Ovocalyxin-36, a novel chicken eggshell protein related to lipolysaccharide-binding proteins (LBP), bactericidal permeability-increasing protein (BPI) and Plunc family proteins. J Biol Chem 282 (8): 5273-5286.
37. Gavrilenko TI, Siurin SA, Lolaeva LT, Sauchenko VM. 1992. The characteristics of lysozyme and carbenicillin action on the clinico-immunological status of patients with chronic bronchitis . Vrachebnoe Delo 8: 42-45.
38. Gerbaux V, Villa A, Monamy C, Bertrand A. 1997. Use of lysozyme to inhibit malolactic fermentation and to stabilize wine after malolactic fermentation. Amer J Enol Vitic 48 (1): 49-54.
39. Goldberg J, Shrikant P, Mescher MF. 2003. In vivo augmentation of tumor-specific CTL responses by class I/peptide antigen complexes on microspheres (large multivalent immunogen). J Immunol 170 (1): 228-235.
40. Guérin-Dubiard C, Nau F. 2007. Minor proteins. In: R Huopalahti, R López-Fandiño, M Anton, R Schade, eds., Bioactive Egg Compounds, 93-98. Berlin: Springer.
41. Guérin-Dubiard C, Pasco M, Hietanen A, Bosque Qd A, Nau F, Croguennec T. 2005. Hen egg white fractionation by ion exchange chromatography. J Chrom A 1090 (1-2): 58-67.
42. Hancock RE, Chapple DS. 1999. Peptide antibiotics. Antimicrob Agents Ch 43 (6): 1317-1323.
43. Hang X, Gerry RH, William EH, Janice MB, Narayan CR. 2002. Effects of ovotransferrin on chicken macrophages and heterophil-granulocytes. Dev Comp Immunol 26 (9): 805-815.
44. He X, Tsang TC, Luo P, Zhang T, Harris DT. 2003. Enhanced tumor immunogenicity through coupling cytokine expression with antigen presentation. Cancer Gene Ther 10 (9): 669-677.
45. Herzog J, Maekawa Y, Cirrito TP, Illian Bs, Unanue ER. 2005. Activated antigen presenting cells select and present chemically modified peptides recognized by unique CD4 T cells. PNAS 102 (22): 7928-7933.
46. Hiidenhovi J. 2007. Ovomucin. In: R Huopalahti, R López-Fandiño, M Anton, R Schade, eds., Bioactive Egg Compounds, 61-66. Berlin: Springer.
47. Hincke MT. 1995. Ovalbumin is a component of the chicken eggshell matrix. Connect Tissue Res 31 (3): 227-233.
48. Hincke MT, Gautron J, Panheleux M, Garcia-Ruiz JM, McKee MD, Nys Y. 2000. Identification and localization of lysozyme as a component of eggshell membranes and eggshell matrix. Matrix Biol 19 (5): 443-453.
49. Hirota Y, Yang MP, Araki S, Yoshihara K, Furusawa S, Yasuda M, Mohamed A, Matsumoto Y, Onodera T. 1995. Enhancing effects of chicken egg white derivatives on the phagocytic response in the dog. J Vet Med Sci 57 (5): 825-829.
50. Hoffman DR. 1983. Immunochemical identification of the allergens in egg white. J Allerg Clin Immunol 71 (5): 481-486.
51. Holen E, Bolann B, Elsayed S. 2001. Novel B and T cell epitopes of chicken ovomucoid (Gal d 1) induce T cell secretion of IL-6, IL-13, and IFN-gamma. Clin Exp Allergy 31 (6): 952-964.
52. Hughey VL, Wilger PA, Johnson EA. 1989. Antibacterial activity of hen white lysozyme against Listeria monocytogens Scott A in foods. Appl Environ Microbiol 55 (3): 631-638.
53. Hunt LT, Dayhoff MO. 1980. A surprising new protein superfamily containing ovalbumin, antithrombin-III, and alpha 1-proteinase inhibitor. Biochem Biophys Res Commun 95 (2): 864-871.
54. Huopalahti R, López-Fandiño R, Anton M, Schade R. 2007. Bioactive Egg Compounds. Berlin: Springer.
55. Ibrahim HR. 1997. Insights into the structure-function relationships of ovalbumin, ovotransferrin, and lysozyme. In: T Yamamoto, LR Juneja, H Hatta, M Kim, eds., Hen Eggs: Their Basic and Applied Science, 37-56. New York: CRC Press.
56. Ibrahim HR, Aoki T, Pellegrini A. 2002. Strategies for new antimicrobial proteins and peptides: Lysozyme and aprotinin aprotenin as model molecules. Curr Pharma Design 8 (9): 671-693.
57. Ibrahim HR, Higashiguchi S, Mamoru Koketsu, Juneja LR, Kim M, Yamamoto T, Sugimoto Y, Aoki T. 1996. Partially unfolded lysozyme at neutral pH agglutinates and kills Gram-negative and Gram-positive bacteria through membrane damage mechanism. J Agric Food Chem 44 (12): 3799-3806.
58. Ibrahim HR, Hoq Md. I, Aoki T. 2007. Ovotransferrin possesses SOD-like superoxide anion scavenging activity that is promoted by copper and manganese binding. Intl J Biol Macromol 41 (5): 631-640.
59. Ibrahim HR, Iwamori E, Sugimoto Y, Aoki T. 1998. Identification of a distinct antibacterial domain within the N-lobe of ovotransferrin . Biochem Biophys Acta 401 (3): 289-303.
60. Ibrahim HR, Kobayashi K, Kato AJ. 1993. Length of hydrocarbon chain and antimicrobial action to Gram-negative bacteria to fatty acylated lysozyme. Agric Food Chem 41 (7): 1164-1168.
61. Ibrahim HR, Sugimoto Y, Aoki T. 2000. Ovotransferrin antimicrobial peptide (OTAP-92) kills bacteria through a membrane damage mechanism. Biochimica et Biophysica Acta (BBA) General Subjects 1523 (2): 196-205.
62. Ibrahim HR, Thomas U, Pellegrini A. 2001. A helix-loop peptide at the upper lip of the active site cleft of lysozyme confers potent antimicrobial activity with membrane permeabilization action. J Bio Chem 276 (47): 43767-43774.
63. Ibrahim HR, Yamada M, Matsushita K, Kobayashi R, Kato A. 1994. Enhanced bactericidal action of lysozyme to Escherichia coli by inserting a hydrophobic pentapeptide into its C terminus . Biol Chem 269 (7): 5059-5063.
64. Ishikawa S, Yano Y, Arihara K, Itoh M. 2004. Egg yolk phosvitin inhibits hydroxyl radical formation from the Fenton reaction. Biosci Biotechnol Biochem 68 (6): 1324-1331.
65. Ito Y, Fujii T. 1962. Chemical compositions of the egg yolk lipoproteins. J Biochem 52 (3): 221-222.
66. Joubert FJ, Cook WH. 1958. Preparation and characterization of phosvitin from hen egg yolk. Can J Biochem Physiol 36 (1): 399-408.
67. Katayama S, Ishikawa SI, Fan MZ, Mine Y. 2007. Oligophosphopeptides derived from egg yolk phosvitin up-regulate γ-glutamylcysteine synthetase and antioxidant enzymes against oxidative stress in Caco-2 cells. J Agric Food Chem 55 (8): 2829-2835.
68. Katayama S, Xu X, Fan MZ, Mine Y. 2006. Antioxidative stress activity of oligosaccharides derived from hen egg yolk phosvitin in Caco-2 cells. J Agric Food Chem 54 (3): 773-778.
69. Kato I, Schrode J, Kohr WJ, Laskowski M Jr. 1987. Chicken ovomucoid: Determination of its amino acid sequence, determination of the trypsin reactive site, and preparation of all three of its domains. Biochem 26 (1): 193-201.
70. Keppler D. 2006. Towards novel anti-cancer strategies based on cystatin function. Cancer Lett 235 (2): 159-176.
71. Kim SE, Kim HH, Kim JY. 2000. Anticancer activity of hydrophobic peptides from soy proteins. BioFactors 12 (1-4): 151-155.
72. Kitamoto T, Nakashima M, Ikai A. 1982. Hen egg white ovomacroglobulin has a protease inhibitory activity. J Biochem 92 (5): 1679-1682.
73. Kobayashi K, Hattori M, Hara-Kudo Y, Okubo T, Yamamoto S, Takita T, Sugita-Konishi Y. 2004. Glycopeptide derived from hen egg ovomucin has the ability to bind enterohemorrhagic Escherichia coli O157: H7. J Agric Food Chem 52 (18): 5740-5746.
74. Kodama Y, Kimura N. 1999. Inhibitor of Helicobacter pylori colonization. U.S. Patent 6 (235): 709 B1.
75. Korant BD, Towatari T, Ivanoff L, Petteway S Jr, Brzin J, Lenarcic B, Turk V. 1986. Viral therapy: Prospects for protease inhibitors. J Cell Biochem 32 (2): 91-95.
76. Korpela J, Salonen EM, Kuusela P, Sarvas M, Vaheri A. 1984. Binding of avidin to bacteria and to the outer membrane porin of Escherichia coli. FEMS Microbiol Lett 22 (1): 3-10.
77. Kovacs-Nolan J, Marshall P, Mine Y. 2005. Advances in the value of egg and egg components for human health. J Agric Food Chem 53 (22): 8421-8431.
78. Kovacs-Nolan J, Mine Y. 2004. Avian egg antibodies: Basic and potential applications. Avian Poultry Biol Rev 15 (1): 25-46.
79. Layman DK. 2003. The role of leucine in weight loss diets and glucose homeostasis. J Nutr 133 (1): 261-267.
80. Lee DG, Hahm KS, Park Y, Kim HY. 2005. Functional and structural characteristics of anticancer peptide Pep27 analogues . Cancer Cell Intl 5 (22): 21-25.
81. Leng B, Liu XD, Chen QX. 2005. Inhibitory effects of anticancer peptide from Mercenaria on the BGC-823 cells and several enzymes. FEBS Lett 579 (5): 1187-1190.
82. Lesnierowski G, Kijowski J. 2007. Lyzozyme. In: R Huopalahti, R López-Fandiño, M Anton, R Schade, eds., Bioactive Egg Compounds, 33-42. Berlin: Springer.
83. Li-Chan E, Nakai S. 1989. Biochemical basis for the properties of egg white. Crit Rev Poult Biol 2 (1): 21-58.
84. Li-Chan ECY, Powrie WD, Nakai S. 1995. The chemistry of eggs and egg products. In: WJ Stadelman, OJ Cotterill, eds., Egg Science and Technology, 4th ed., 105-175. New York: Haworth Press.
85. Liu H, Zheng F, Cao Q, Ren B, Zhu L, Striker G, Vlassara H. 2006. Amelioration of oxidant stress by the defensin lysozyme. Amer J Physiol Endocrinol Metab 290 (5): E824-832.
86. López-Fandiño R, Recio I, Ramos M. 2007. Egg-protein derived peptides with antihypertensive activity. Physiological, chemical and technological aspects of milk-protein-derived peptides with antihypertensive and ACE-inhibitory activity. In: R Huopalahti, R López-Fandiño, M Anton, R Schade, eds., Bioactive Egg Compounds, 199-211. Berlin: Springer.
87. Losso JN, Nakai S, Charter EA. 2000. Lysozyme. In: AS Naidu, ed., Natural Food Antimicrobial Systems, 185-210. New York: CRC Press.
88. Lu CL, Baker RC. 1986. Characteristics of egg yolk phosvitin as an antioxidant for inhibiting metalcatalyzed phospholipid oxidation . Poult Sci 65 (11): 2065-2073.
89. Maeda H, Akaike T, Sakata Y, Maruo K. 1993. Role of bradykinin in microbial infection: Enhancement of septicemia by microbial proteases and kinin. Agents Actions Suppl 42 (1): 159-165.
90. Majumder K, Wu J. 2009. Angiotensin I converting enzyme inhibitory peptides from simulated in vitro gastrointestinal digestion of cooked eggs. J Agric Food Chem 57 (2): 471-477.
91. Majumder K, Wu J. 2010. A new approach for identification of novel antihypertensive peptides from egg proteins by QSAR and bioinformatics. Food Res Int (in press).
92. Mann J, Truswell AS. 1998. Essentials of Human Nutrition. New York: Oxford University Press.
93. Maruo K, Akaike T, Ono T, Maeda H. 1998. Involvement of bradykinin generation in intravascular dissemination of Vibrio vulnificus and prevention of invasion by a bradykinin antagonist . Infect Immun 66 (2): 866-869.
94. Matoba N, Usui H, Fujita H, Yoshikawa M. 1999. A novel anti-hypertensive peptide derived from ovalbumin induces nitric oxide-mediated vasorelaxation in an isolated SHR mesenteric artery. FEBS Lett 452 (3): 181-184.
95. Mellet P, Michels B, Bieth JG. 1996. Heat-induced conversion of ovalbumin into a proteinase inhibitor. J Biol Chem 271 (48): 30311-30314.
96. Messens W, Grijspeerdt K, Herman L. 2005. Eggshell penetration by Salmonella: A review. World's Poult Sci J 61 (5): 71-85.
97. Miguel M, Aleixandre A. 2006. Recent advances in nutritional science. J Nutr 136 (6): 1457-1460.
98. Miguel M, López-Fandiño R, Ramos M, Aleixandre MA. 2005. Short-term effect of egg white hydrolysate products on the arterial blood pressure of hypertensive rats. Brit J Nutr 94 (2): 731-737.
99. Miguel M, Recio I, Gómez-Ruiz JA, Ramos M, López-Fandiño R. 2004. Angiotensin I-converting enzyme inhibitory activity of peptides derived from egg white proteins by enzymatic hydrolysis. J Food Prote 67 (9): 1914-1920.
100. Millward DJ. 2004. Macronutrient intakes as determinants of dietary protein and amino acid adequacy. J Nutr 134 (6): 1588-1596.
101. Milner JA. 2004. Molecular targets for bioactive food components . J Nutr 134 (9): 2492-2498.
102. Mine Y. 2007. Egg proteins and peptides in human health-chemistry, bioactivity and production. Curr Pharm Des 13 (9): 875-884.
103. Mine Y, Ma F, Lauriau S. 2004. Antimicrobial peptides released by enzymatic hydrolysis of hen egg white lysozyme. J Agric Food Chem 52 (5): 1088-1094.
104. Miyagawa S, Kamata R, Matsumoto K, Okamura R, Maeda H. 1994. Therapeutic intervention with chicken egg white ovomacroglobulin and a new quinolone on experimental Pseudomonas keratitis. Graefe's Arch Clin Exp 232 (8): 488-493.
105. Molla A, Matsumura Y, Yamamoto T, Okamura R, Maeda H. 1987. Pathogenic capacity of proteases from Serratia marcescens and Pseudomonas aeruginosa and their suppression by chicken egg white ovomacroglobulin. Infect Immun 55 (10): 2509-2517.
106. Muehlenweg B, Assfalg-Machleidt I, Parrado SG, Burgle M, Creutzburg S, Schmitt M, Auerswald EA, Machleidt W, Magdolen V. 2000. A novel type of bifunctional inhibitor directed against proteolytic activity and receptor/ligand interaction. Cystatin with a urokinase receptor binding site. J Biol Chem 275 (43): 33562-33566.
107. Nakai S. 2000. Molecular modifications of egg proteins for functional improvement. In: JS Sim, S Nakai, W Guenter, eds., Egg Nutrition and Biotechnology, 205-217. Wallingford, UK: CAB International.
108. Nakamura NK, Furukawa N, Matsuoka M, Takahashi T, Yamanaka Y. 1997. Enzyme activity of lysozyme-dextran complex prepared by high pressure treatment. Food Sci Tech Inter 3 (2): 235-238.
109. Nakamura S, Ogawa M, Nakai S, Kato A, Kitts DD. 1998. Antioxidant activity of a Maillard-type phosvitin-galactomannan conjugate with emulsifying properties and heat stability. J Agric Food Chem 46 (10): 3958-3963.
110. Nielson KL, Sottrup-Jensen NL, Nagase H, Etzerodt M. 1994. The primary structure of ovomacroglobulin. Ann NY Acad Sci 737: 476-479.
111. Oguro T, Ohaki Y, Asano G, Ebina T, Watanabe K. 2001. Ultrastructural and immunohistochemical characterization on the effect of ovomucin in tumor angiogenesis. Jpn J Clin Electron Microsc 33 (2): 89-99.
112. Ondetti MA, Rubin B, Cushman DW. 1977. Design of specific inhibitors of angiotensin converting enzyme: New class of orally active antihypertensive agents. Science 196 (4288): 441-444.
113. Otani H, Odashima M. 1997. Inhibition of proliferative responses of mouse spleen lymphocytes by lacto-and ovotransferrins. Food Agric Immunol 9: 193-202.
114. Park PJ, Jung WK, Nam KS, Shahidi F, Kim SK. 2001. Purification and characterization of antioxidative peptides from protein hydrolysate of lecithin-free egg yolk. JAOCS 78 (6): 651-656.
115. Parker B, Noakes M, Luscombe N, Clifton P. 2002. Effect of a high-protein, monounsaturated fat weight loss diet on glycemic control and lipid levels in type 2 diabetes. Diabetes Care 25 (3): 425-430.
116. Pellegrini A. 2003. Antimicrobial peptides from food proteins. Curr Pharma Design 9 (16): 1225-1238.
117. Pellegrini A, Hulsmeier AJ, Hunziker P, Thomas U. 2004. Proteolytic fragments of ovalbumin display antimicrobial activity. Biochimica et Biophysica Acta 1672 (2): 76-85.
118. Pellegrini A, Thomas U, Bramaz N, Klauser S, Hunziker P, Fellenberg R von. 1997. Identification and isolation of bactericidal domain in chicken egg white lysozyme. J Appl Microbio 82 (3): 372-378.
119. Pellegrini A, Thomas U, Fellenberg R von, Wild P. 1992. Bactericidal activity of lysozyme and aprotinin against Gram-negative and Gram-positive bacteria related to their basic character. J Appl Bacterio 72 (3): 180-187.
120. Premzl A, Puizdar V, Zavasnik-Bergant V, Kopitar-Jerala N, Lah TT, Katunuma N, Sloane BF, Turk V, Kos J. 2001. Invasion of ras-transformed breast epithelial cells depends on the proteolytic activity of cysteine and aspartic proteinases. Biol Chem 382 (5): 853-857.
121. Proctor VA, Cunningham FF. 1988. The chemistry of lysozyme and its use as a food preservative and a pharmaceutical. Crit Rev Food Sci Nutr 26 (4): 359-395.
122. Rehault S. 2007. Proteases. In: R Huopalahti, R López-Fandiño, M Anton, R Schade, eds., Bioactive Egg Compounds, 81-83. Berlin: Springer.
123. Sato K, Inaba Y, Shinozaki T, Fujii R, Matsumoto M. 1981. Isolation of human rotavirus in cell cultures. Arch Virol 69 (2): 155-160.
124. Sattar Khan MA, Nakamura S, Ogawa M, Akita E, Kato E. 2000. Bacterial action of egg yolk phosvitin against Escherichia coli under thermal stress. J Agric Food Chem 48 (5): 1503-1506.
125. Sava G. 1996. Pharmacological aspects and therapeutic applications of lysozymes. EXS 75 (1): 433-449.
126. Sava G, Benetti A, Ceschia V, Pacor S. 1989. Lysozyme and cancer: Role of exogenous lysozyme as anticancer agent (review). Anticancer Res 9 (3): 583-591.
127. Saxena I, Tayyab S. 1979. Protein proteinase inhibitors from avian egg whites. Cell Mol Life Sci 53 (1): 13-23.
128. Scott D, Hammer FE, Szalkucki TJ. 1987. Bioconversions: Enzyme technology. In: D Knorr, ed., Food Biotechnology, 413-442. New York: Marcel Dekker.
129. Shah RB, Khan MA. 2004. Protection of salmon calcitonin breakdown with serine proteases by various ovomucoid species for oral drug delivery. J Pharm Sci 93 (2): 392-406.
130. Sharon N, Ofek I. 2002. Fighting infectious diseases with inhibitors of microbial adhesion to host tissues. Crit Rev Food Sci Nutr 42 (3): 267-272.
131. Stein PE, Leslie AG, Finch JT, Turnell WJ, McLaughlin PJ, Carrell RW. 1990. Crystal structure of ovalbumin as a model for the reactive centre of serpins. Nature 347 (6288): 99-102.
132. Sugimoto Y, Sanuki S, Ohsako S, Higashimoto Y, Kondo M, Kurawaki J, Ibrahim HR, Aoki T, Kusakabe T, Koga K. 1999. Ovalbumin in developing chicken eggs migrates from egg white to embryonic organs while changing its conformation and thermal stability. J Biol Chem 274 (16): 11030-11037.
133. Sugita-Konishi Y, Sakanaka S, Sasaki K, Juneja LR, Noda T, Amano F. 2002. Inhibition of bacterial adhesion and Salmonella infection in BALB/c mice by sialyloligosaccharides and their derivatives from chicken egg yolk. J Agric Food Chem 50 (12): 3607-3613.
134. Sunwoo HH, Sadeghi G, Karami H. 2006. Natural anti-microbial egg yolk antibody. In: JS Sim, HH Sunwoo, eds., The Amazing Egg: Nature's Perfect Functional Food for Health, 295-325. Edmonton: University of Alberta Publishing.
135. Taborsky G. 1983. Phosvitin. Adv Inorg Biochem 5 (2): 235-279.
136. Tanizaki H, Tanaka H, Iwata H, Kato A. 1997. Activation of macrophages by sulfated glycopeptides in ovomucin, yolk membrane, and chalazae in chicken eggs. Biosci Biotechnol Biochem 61 (11): 1883-1889.
137. Tenuovo J. 2002. Clinical applications of antimicrobial host proteins lactoperoxidase, lysozyme and lactoferrin in xerostomia: Efficacy and safety. Oral Dis 8 (1): 23-29.
138. Tomimatsu Y, Clary JJ, Bartulovich JJ. 1966. Physical characterization of ovoinhibitor, a trypsin and chymotrypsin inhibitor from chicken egg white. Arch Biochem Biophys 115 (3): 536-544.
139. Tsuge N, Eikawa Y, Nomura Y, Yamamoto M, Sugisawa K. 1991. Antioxidative activity of peptides prepared by enzymatic hydrolysis of egg white albumin. Nippon Nogeikagaku Kaisi 65 (2): 1635-1641.
140. Tsuge Y, Shimoyamada M, Watanabe K. 1996. Binding of egg white proteins to viruses. Biosci Biotech Biochem 60 (7): 1503-1504.
141. Valenti P, Antonini G, Von Hunolstein C, Visca P, Orsi N, Antonini E. 1983. Studies of the antimicrobi al activity of ovotransferrin . Intl J Tissue React 5 (1): 97-105.
142. Valenti P, Visca P, Antonini G, Orsi N. 1985. Antifungal activity of ovotransferrin towards genus Candida. Mycopathologia 89 (3): 169-175.
143. 2+ and other metal ions on the antibacterial activity of ovotransferrin. Med Microbio Immunol 176 (3): 123-130.
144. Vander Wal JS, Gupta A, Khosla P, Dhurandhar NV. 2008. Egg breakfast enhances weight loss. Inter J Obes 32 (10): 1545-1551.
145. Vannini L, Lanciotti R, Baldi D, Guerzoni ME. 2004. Interaction between high pressure homogenization and antimicrobial activity of lysozyme and lactoperoxidase. Intl J Food Microbio 94 (2): 123-135.
146. Verdot L, Lalmanach G, Vercruysse V, Hartmann S, Lucius R, Hoebeke J. 1999. Chicken cystatin stimulates nitric oxide release from interferon-g-activated mouse peritoneal macrophages via cytokine synthesis. Eur J Biochem 266 (3): 1111-1117.
147. Vidovic D, Graddis T, Chen F, Slagle P, Diegel M, Stepan L, Laus R. 2002. Antitumor vaccination with HER-2-derived recombinant antigens. Intl J Cancer 102 (6): 660-664.
148. Watanabe K, Shimoyamada M, Onizuka T, Akiyama H, Niwa M, Ido T, Tsuge Y. 2004. Amino acid sequence of alpha-subunit in hen egg white ovomucin deduced from cloned cDNA. DNA Seq 15 (4): 251-261.
149. Watanabe K, Tsuge Y, Shimoyamada M, Ogama N, Ebina T. 1998. Antitumor effects of Pronase-treated fragments, glycopeptides, from ovomucin in hen egg white in a double grafted tumor system. J Agric Food Chem 46 (8): 3033-3038.
150. Whisstock J, Skinner R, Lesk AM. 1998. An atlas of serpin conformations. Trends in Biochem Sci 23 (2): 63-67.
151. Wilde CG, Griffith JE, Marra MN, Snable JL, Scott RW. 1989. Purification and characterization of human neutrophil peptide 4, a novel member of the defensin family. J Biol Chem 264 (19): 11200-11203.
152. Wu J, Akaike T, Hayashida K, Okamoto T, Okuyama A, Maeda H. 2001. Enhanced vascular permeability in solid tumor involving peroxynitrite and matrix metalloproteinases. Jpn J Cancer Res 92 (4): 439-451.
153. Wu J, Aluko RE, Nakai S. 2006. Structural requirements of angiotensin I converting enzyme inhibitory peptides: Quantitative structure-activity relationships study of di-and tripeptides. J Agric Food Chem 54 (3): 732-738.
154. Xu X, Katayama S, Mine Y. 2007. Antioxidant activity of tryptic digests of hen egg yolk phosvitin. J Sci Food Agric 87 (14): 2604-2608.
155. Yet MG, Wold F. 1990. The distribution of glycan structures in individual N-glycosylation sites in animal and plant glycoproteins . Arch Biochem Biophys 278 (2): 356-364.