New strategy for expression of recombinant hydroxylated human-derived gelatin in Pichia pastoris KM71

Journal of Agricultural and Food Chemistry

Volumn 59, Issue 13, 2011, Pages 7127-7134

  Duan, Huiming ^a   Umar, Sirajo ^a   Xiong, Runsong ^a   Chen, Jinchun ^a  

^a BEIJING UNIVERSITY OF CHEMICAL TECHNOLOGY   (China)

Author keywords

coexpression; hydroxylation; Pichia pastoris KM71; prolyl 4 hydroxylase; recombinant gelatin

Indexed keywords

ALCOHOL OXIDASE 1 PROMOTER; CO-EXPRESSION; COMPOSITION ANALYSIS; EXPRESSION CASSETTE; EXPRESSION VECTORS; FOOD INDUSTRIES; FORMALDEHYDE DEHYDROGENASE; GEL ELECTROPHORESIS; GELLING AGENTS; HISTORY OF USE; INDUCIBLE PROMOTER; NEW STRATEGY; P. PASTORIS; PICHIA PASTORIS; PICHIA PASTORIS KM71; PROLINE RESIDUES; PROLYL-4-HYDROXYLASE; PROTEIN DISULFIDE ISOMERASES; RECOMBINANT GELATIN; SUBUNIT GENES; TANDEM MASS SPECTROMETRY; UV ABSORPTION;

ABSORPTION SPECTROSCOPY; AMINO ACIDS; ELECTROPHORESIS; GENES; LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; METHANOL; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; RESONANCE; SODIUM; YEAST;

HYDROXYLATION;

GELATIN; PROCOLLAGEN PROLINE 2 OXOGLUTARATE 4 DIOXYGENASE; PROTEIN DISULFIDE ISOMERASE; RECOMBINANT DNA; RECOMBINANT PROTEIN;

ARTICLE; CHEMISTRY; GENE EXPRESSION; GENE VECTOR; GENETICS; HUMAN; HYDROXYLATION; METABOLISM; PICHIA;

DNA, RECOMBINANT; GELATIN; GENE EXPRESSION; GENETIC VECTORS; HUMANS; HYDROXYLATION; PICHIA; PROCOLLAGEN-PROLINE DIOXYGENASE; PROTEIN DISULFIDE-ISOMERASES; RECOMBINANT PROTEINS;

PICHIA PASTORIS;

EID: 79960051943     PISSN: 00218561     EISSN: 15205118     Source Type: Journal    
DOI: 10.1021/jf200778r     Document Type: Article

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