Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris

BMC Biotechnology

Volumn 11, Issue , 2011, Pages

  Nordén, Kristina ^a   Agemark, Maria ^a   Danielson, Jonas T ^a   Alexandersson, Erik ^a,b   Kjellbom, Per ^a   Johanson, Urban ^a  

^a LUND UNIVERSITY   (Sweden)

^b STELLENBOSCH UNIVERSITY   (South Africa)

Author keywords

Major Intrinsic Proteins; Pichia pastoris aquaporins; qPCR

Indexed keywords

AQUAPORINS; EUKARYOTIC MEMBRANE PROTEIN; EUKARYOTIC PROTEINS; EXPRESSION LEVELS; EXPRESSION SYSTEM; GENE DOSAGE; HETEROLOGOUS EXPRESSION; HIGH YIELD; INSECT CELLS; ISOFORMS; LOCALISATION; MAJOR INTRINSIC PROTEINS; MEMBRANE PROTEINS; NATURAL SOURCES; OVER-EXPRESSION; P. PASTORIS; PICHIA PASTORIS; POST-TRANSCRIPTIONAL; PROTEIN YIELD; QPCR; RECOMBINANT EXPRESSION; RECOMBINANT PROTEIN EXPRESSION; ROBUST METHODS; STRUCTURAL STUDIES; TRANSCRIPT LEVEL;

BIOLOGICAL MEMBRANES; GLYCOPROTEINS; MEMBRANES; POLYMERASE CHAIN REACTION; PROTEIN FOLDING; PROTEINS; TISSUE; YEAST;

GENE EXPRESSION;

AQUAPORIN; ISOPROTEIN; MESSENGER RNA; ARABIDOPSIS PROTEIN; RECOMBINANT PROTEIN;

ARTICLE; CORRELATION ANALYSIS; GENE DOSAGE; MOLECULAR CLONING; PICHIA PASTORIS; POLYMERASE CHAIN REACTION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN LOCALIZATION; QUANTITATIVE ANALYSIS; SEQUENCE ANALYSIS; GENE EXPRESSION REGULATION; GENETIC VARIABILITY; GENETICS; HUMAN; METABOLISM; PICHIA; RNA PROCESSING;

EUKARYOTA; HEXAPODA; PICHIA; PICHIA PASTORIS;

AQUAPORINS; ARABIDOPSIS PROTEINS; CLONING, MOLECULAR; GENE DOSAGE; GENE EXPRESSION REGULATION, FUNGAL; GENETIC VARIATION; HUMANS; PICHIA; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS; RNA PROCESSING, POST-TRANSCRIPTIONAL; RNA, MESSENGER;

EID: 79955746703     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-47     Document Type: Article

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