Evaluation of Mut+ and MutS Pichia pastoris phenotypes for high level extracellular scFv expression under feedback control of the methanol concentration

Biotechnology Progress

Volumn 22, Issue 3, 2006, Pages 881-888

  Pla, Itzcoatl A ^a   Damasceno, Leonardo M ^b   Vannelli, Todd ^b   Ritter, Gerd ^c   Batt, Carl A ^b   Shuler, Michael L ^a  

^a Cornell University   (United States)

^b Department of Obstetrics Gynecology   (United States)

^c LUDWIG INSTITUTE FOR CANCER RESEARCH   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODIES; BIOMASS; BIOREACTORS; CELLS; CONCENTRATION (PROCESS); FEEDBACK CONTROL; FERMENTATION; GENES; ORGANIC COMPOUNDS; PROTEINS; SUBSTRATES;

EXTRACELLULAR SECRETION; FERMENTATION SCHEMES; INDUCTION DENSITY; PICHIA PASTORIS PHENOTYPES;

METHANOL;

PICHIA PASTORIS;

METHANOL; MONOCLONAL ANTIBODY;

ARTICLE; BIOLOGICAL MODEL; BIOREACTOR; BIOSYNTHESIS; COMPARATIVE STUDY; DRUG EFFECT; FEEDBACK SYSTEM; FERMENTATION; GENETICS; IMMUNOGLOBULIN VARIABLE REGION; METABOLISM; PHENOTYPE; PICHIA; TIME;

ANTIBODIES, MONOCLONAL; BIOREACTORS; FEEDBACK, BIOCHEMICAL; FERMENTATION; IMMUNOGLOBULIN VARIABLE REGION; METHANOL; MODELS, BIOLOGICAL; PHENOTYPE; PICHIA; TIME FACTORS;

EID: 33745037971     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp060012+     Document Type: Article

References (26)

1. Werten, M. W. T.; Van den Bosch, T. J.; Wind, R. D.; Mooibroek, H.; De Wolf, F. A. High-yield secretion of recombinant gelatins by Pichia pastoris. Yeast 1999, 15 (11), 1087-1096.
2. Barendswaard, E. C.; Scott, A. M.; Divgi, C. R.; Williams, C.; Coplan, K.; Riedel, E.; Yao, T. J.; Gansow, O. A.; Finn, R. D.; Larson, S. M.; Old, L. J.; Welt, S. Rapid and specific targeting of monoclonal antibody A33 to a colon cancer xenograft in nude mice. Int. J. Oncol. 1998, 12 (1), 45-53.
3. Welt, S.; Scott, A. M.; Divgi, C. R.; Kemeny, N.; Finn, R. D.; Daghighian, F.; Germain, J. S.; Richards, E. C.; Larson, S. M.; Old, L. J. Phase I/II study of iodine 125-labeled monoclonal antibody A33 in patients with advanced colon cancer. J. Clin. Oncol. 1996, 14 (6), 1787-1797.
4. Welt, S.; Ritter, G.; Williams, C. J.; Cohen, L.; John, M.; Jungbluth, A.; Richards, E.; Old, L. J.; Kemeny, N. Phase I study of anticolon cancer humanized antibody A33. Clin. Cancer Res. 2003, 9, 1338-1346.
5. Ritter, G.; Cohen, L. S.; Williams, C.; Richards, E. C.; Old, L. J.; Welt, S. Serological analysis of human anti-human antibody responses in colon cancer patients treated with repeated doses of humanized monoclonal antibody A33. Cancer Res. 2001, 61 (18), 6851-6859.
6. Rader, C.; Ritter, G.; Nathan, S.; Elia, M.; Gout, I.; Jungbluth, A.; Cohen, L.; Welt, S.; Old, L. J.; Barbas, Cr. The rabbit antibody repertoire as a novel source for the generation of therapeutic human antibodies. J. Biol. Chem. 2000, 275, 13668-13676.
7. Cregg, J. M. Expression in the methylotrophic yeast Pichia pastoris. In Gene Expression Systems: Using Nature for the An of Expression; Academic Press: New York, 1999; pp 157-190.
8. Hellwig, S.; Emde, F.; Raven, N. P. G.; Henke, M.; van der Logt, P.; Fischer, R. Analysis of single-chain antibody production in Pichia pastoris using on-line methanol control in fed-batch and mixed-feed fermentations. Biotechnol. Bioeng. 2001, 74 (4), 344-352.
9. Pichia Fermentation Process Guidelines; Invitrogen: San Diego, CA, 1999.
10. Guarna, M. M.; Lesnicki, G. J.; Ram, B. M.; Robinson, J.; Radziminski, C. Z.; Hasenwinkle, D.; Boraston, A.; Jervis, E.; MacGillivray, R. T. A.; Turner, R. F. B.; Kilburn, D. G. On-line monitoring and control of methanol concentration in shake-flask cultures of Pichia pastoris. Biotechnol. Bioeng. 1997, 56 (3), 279-286.
11. Kupcsulik, B.; Sevella, B.; Ballagi, A.; Kozma, J. Evaluation of three methanol feed strategies for recombinant Pichia pastoris Mut-(s) fermentation. Acta Alimentaria 2001, 30 (1), 99-111.
12. Chiruvolu, V.; Cregg, J. M.; Meagher, M. M. Recombinant protein production in an alcohol oxidase-defective strain of Pichia pastoris in fedbatch fermentations. Enzyme Microb. Technol. 1997, 21 (4), 277-283.
13. Zhang, W. H.; Bevins, M. A.; Plantz, B. A.; Smith, L. A.; Meagher, M. M. Modeling Pichia pastoris growth on methanol and optimizing the production of a recombinant protein, the heavy-chain fragment C of botulinum neurotoxin, serotype A. Biotechnol. Bioeng. 2000, 70(1), 1-8.
14. Chung, J. D. Design of metabolic feed controllers: Application to high- density fermentations of Pichia pastoris. Biotechnol. Bioeng. 2000, 68 (3), 298-307.
15. d'Anjou, M. C.; Daugulis, A. J. A rational approach to improving productivity in recombinant Pichia pastoris fermentation. Biotechnol. Bioeng. 2001, 72 (1), 1-11.
16. Curvers, S.; Brixius, P.; Klauser, T.; Thommes, J.; Weuster-Botz, D.; Takors, R.; Wandry, C. Human chymotrypsinogen B production with Pichia pastoris by integrated development of fermentation and downstream processing. Part 1. fermentation. Biotechnol. Prog. 2001, 17 (3), 495-502.
17. Minning, S.; Serrano, A.; Ferrer, P.; Sola, C.; Schmid, R. D.; Valero, F. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J. Biotechnol. 2001, 86 (1), 59-70.
18. Zhang, W. H.; Smith, L. A.; Plantz, B. A.; Schlegel, V. L.; Meagher, M. M. Design of methanol feed control in Pichia pastoris fermentations based upon a growth model. Biotechnol. Prog. 2002, 18(6), 1392-1399.
19. Pla, I.; Shuler, M. L. Production of Antibody Fragments and Antigens via the Pichia Pastoris Expression System. AIChE Annual Meeting, 2003.
20. Damascene, L.; Pla, I.; Chang, H.-J.; Cohen, L.; Ritter, G.; Old, L. J.; Batt, C. An optimized fermentation process for high-level production of a single-chain Fv Antibody fragment in Pichia pastoris. Protein Expression Purif. 2004, 37, 18-26.
21. Higgins, D.; Cregg, J. M. Pichia Protocols; Humana Press: London, 1998.
22. Sambrook, J.; Fritsch, E. F.; Maniatis, T. Molecular Cloning. A Laboratory Manual, 2nd ed.; Cold Spring Harbor Laboratory Press: Plainview, NY, 1989; Vol. 2.
23. Ogata, K. Modern Control Engineering, 4th ed.; Prentice-Hall: Englewood Cliffs, NJ, 1996; p 997.
24. Horn, U.; Strittmatter, W.; Krebber, A.; Knupfer, U.; Kujau, M.; Wenderoth, R.; Muller, K.; Matzku, S.; Pluckthun, A.; Riesenberg, D. High volumetric yields of functional dimeric miniantibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under nonlimited growth conditions. Appl. Microbiol. Biotechnol. 1996, 46 (5-6), 524-532.
25. Eldin, P.; Pauza, M. E.; Hieda, U.; Lin, G. F.; Murtaugh, M. P.; Pentel, P. R.; Pennell, C. A. High-level secretion of two antibody single chain Fv fragments by Pichia pastoris. J. Immunol. Methods 1997, 201 (1), 67-75.
26. Freyre, F. M.; Vazquez, J. E.; Ayala, M.; Canaan-Haden, L.; Bell, H.; Rodriquez, I.; Gonzalez, A.; Cintado, A.; Gavilondo, J. V. Very high expression of an anti-carcinoembryonic antigen single chain Fv antibody fragment in the yeast Pichia pastoris. J. Biotechnol. 2000, 76 (2-3), 157-163.