Analysis of lipid droplet dynamics and functions in drosophila melanogaster

Methods in Cell Biology

Volumn 116, Issue , 2013, Pages 53-69

  Lee, Han ^a   Peng, Ying ^b   Guo, Yi ^a,b  

^a MAYO CLINIC   (United States)

^b MAYO GRADUATE SCHOOL   (United States)

Author keywords

Drosophila melanogaster; Fat body; Genome wide RNA interference screen; Lipid droplet

Indexed keywords

FAT DROPLET; PHOSPHOLIPID;

ANIMAL; ARTICLE; CELL INCLUSION; CELL ORGANELLE; CHEMISTRY; DROSOPHILA MELANOGASTER; FAT BODY; GENETICS; GENOME-WIDE RNA INTERFERENCE SCREEN; GENOMICS; ISOLATION AND PURIFICATION; LIPID METABOLISM; RNA INTERFERENCE; TRANSGENIC ANIMAL;

DROSOPHILA MELANOGASTER; FAT BODY; GENOME-WIDE RNA INTERFERENCE SCREEN; LIPID DROPLET;

ANIMALS; ANIMALS, GENETICALLY MODIFIED; DROSOPHILA MELANOGASTER; GENOMICS; INCLUSION BODIES; LIPID METABOLISM; ORGANELLES; PHOSPHOLIPIDS; RNA INTERFERENCE;

EID: 84884932544     PISSN: 0091679X     EISSN: None     Source Type: Book Series    
DOI: 10.1016/B978-0-12-408051-5.00004-8     Document Type: Chapter

References (58)

1. Aguila J.R., Hoshizaki D.K., Gibbs A.G. Contribution of larval nutrition to adult reproduction in Drosophila melanogaster. Journal of Experimental Biology 2013, 216(Pt 3):399-406.
2. Aguila J.R., Suszko J., Gibbs A.G., Hoshizaki D.K. The role of larval fat cells in adult Drosophila melanogaster. Journal of Experimental Biology 2007, 210(Pt 6):956-963.
3. Arrese E.L., Rivera L., Hamada M., Mirza S., Hartson S.D., Weintraub S., et al. Function and structure of lipid storage droplet protein 1 studied in lipoprotein complexes. Archives of Biochemistry and Biophysics 2008, 473(1):42-47.
4. Arrese E.L., Soulages J.L. Insect fat body: Energy, metabolism, and regulation. Annual Review of Entomology 2010, 55:207-225.
5. Asha H., Nagy I., Kovacs G., Stetson D., Ando I., Dearolf C.R. Analysis of Ras-induced overproliferation in Drosophila hemocytes. Genetics 2003, 163(1):203-215.
6. Baehrecke E.H. Ecdysone signaling cascade and regulation of Drosophila metamorphosis. Archives of Insect Biochemistry and Physiology 1996, 33(3-4):231-244.
7. Baker K.D., Thummel C.S. Diabetic larvae and obese flies-emerging studies of metabolism in Drosophila. Cell Metabolism 2007, 6(4):257-266.
8. Beller M., Bulankina A.V., Hsiao H.H., Urlaub H., Jackle H., Kuhnlein R.P. PERILIPIN-dependent control of lipid droplet structure and fat storage in Drosophila. Cell Metabolism 2010, 12(5):521-532.
9. Beller M., Sztalryd C., Southall N., Bell M., Jackle H., Auld D.S., et al. COPI complex is a regulator of lipid homeostasis. PLoS Biology 2008, 6(11):e292.
10. Benes H., Spivey D.W., Miles J., Neal K., Edmondson R.G. Fat-body-specific expression of the Drosophila Lsp-2 gene. SAAS Bulletin, Biochemistry and Biotechnology 1990, 3:129-133.
11. Berardinelli W. An undiagnosed endocrinometabolic syndrome: Report of 2 cases. Journal of Clinical Endocrinology and Metabolism 1954, 14(2):193-204.
12. Bettencourt-Dias M., Goshima G. RNAi in Drosophila S2 cells as a tool for studying cell cycle progression. Methods in Molecular Biology 2009, 545:39-62.
13. Bi J., Xiang Y., Chen H., Liu Z., Gronke S., Kuhnlein R.P., et al. Opposite and redundant roles of the two Drosophila perilipins in lipid mobilization. Journal of Cell Science 2012, 125(Pt 15):3568-3577.
14. Billeter J.C., Atallah J., Krupp J.J., Millar J.G., Levine J.D. Specialized cells tag sexual and species identity in Drosophila melanogaster. Nature 2009, 461(7266):987-991.
15. Bourouis M., Jarry B. Vectors containing a prokaryotic dihydrofolate reductase gene transform Drosophila cells to methotrexate-resistance. EMBO Journal 1983, 2(7):1099-1104.
16. Brasaemle D.L. Thematic review series: Adipocyte biology. The perilipin family of structural lipid droplet proteins: Stabilization of lipid droplets and control of lipolysis. Journal of Lipid Research 2007, 48(12):2547-2559.
17. Carvalho M., Sampaio J.L., Palm W., Brankatschk M., Eaton S., Shevchenko A. Effects of diet and development on the Drosophila lipidome. Molecular Systems Biology 2012, 8:600.
18. Chien C.H., Chen W.W., Wu J.T., Chang T.C. Label-free imaging of Drosophila in vivo by coherent anti-Stokes Raman scattering and two-photon excitation autofluorescence microscopy. Journal of Biomedical Optics 2011, 16(1):016012.
19. Colombani J., Raisin S., Pantalacci S., Radimerski T., Montagne J., Leopold P. A nutrient sensor mechanism controls Drosophila growth. Cell 2003, 114(6):739-749.
20. Dietzl G., Chen D., Schnorrer F., Su K.C., Barinova Y., Fellner M., et al. A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila. Nature 2007, 448(7150):151-156.
21. D'Souza-Schorey C., Chavrier P. ARF proteins: Roles in membrane traffic and beyond. Nature Reviews Molecular Cell Biology 2006, 7(5):347-358.
22. Fei W., Du X., Yang H. Seipin, adipogenesis and lipid droplets. Trends in Endocrinology and Metabolism 2011, 22(6):204-210.
23. Fei W., Li H., Shui G., Kapterian T.S., Bielby C., Du X., et al. Molecular characterization of seipin and its mutants: Implications for seipin in triacylglycerol synthesis. Journal of Lipid Research 2011, 52(12):2136-2147.
24. Fukumoto S., Fujimoto T. Deformation of lipid droplets in fixed samples. Histochemistry and Cell Biology 2002, 118(5):423-428.
25. Goshima G. Assessment of mitotic spindle phenotypes in Drosophila S2 cells. Methods in Cell Biology 2010, 97:259-275.
26. Goshima G., Wollman R., Goodwin S.S., Zhang N., Scholey J.M., Vale R.D., et al. Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 2007, 316(5823):417-421.
27. Gronke S., Muller G., Hirsch J., Fellert S., Andreou A., Haase T., et al. Dual lipolytic control of body fat storage and mobilization in Drosophila. PLoS Biology 2007, 5(6):e137.
28. Guo Y., Walther T.C., Rao M., Stuurman N., Goshima G., Terayama K., et al. Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Nature 2008, 453(7195):657-661.
29. Gutierrez E., Wiggins D., Fielding B., Gould A.P. Specialized hepatocyte-like cells regulate Drosophila lipid metabolism. Nature 2007, 445(7125):275-280.
30. Hayes S.A., Miller J.M., Hoshizaki D.K. serpent, a GATA-like transcription factor gene, induces fat-cell development in Drosophila melanogaster. Development 2001, 128(7):1193-1200.
31. Holm C., Kirchgessner T.G., Svenson K.L., Fredrikson G., Nilsson S., Miller C.G., et al. Hormone-sensitive lipase: Sequence, expression, and chromosomal localization to 19 cent-q13.3. Science 1988, 241(4872):1503-1506.
32. Holst L.S., Langin D., Mulder H., Laurell H., Grober J., Bergh A., et al. Molecular cloning, genomic organization, and expression of a testicular isoform of hormone-sensitive lipase. Genomics 1996, 35(3):441-447.
33. Hoshizaki D.K., Lunz R., Ghosh M., Johnson W. Identification of fat-cell enhancer activity in Drosophila melanogaster using P-element enhancer traps. Genome 1995, 38(3):497-506.
34. Jenkins C.M., Mancuso D.J., Yan W., Sims H.F., Gibson B., Gross R.W. Identification, cloning, expression, and purification of three novel human calcium-independent phospholipase A2 family members possessing triacylglycerol lipase and acylglycerol transacylase activities. Journal of Biological Chemistry 2004, 279(47):48968-48975.
35. Krahmer N., Guo Y., Wilfling F., Hilger M., Lingrell S., Heger K., et al. Phosphatidylcholine synthesis for lipid droplet expansion is mediated by localized activation of CTP:phosphocholine cytidylyltransferase. Cell Metabolism 2011, 14(4):504-515.
36. Kuhnlein R.P. The contribution of the Drosophila model to lipid droplet research. Progress in Lipid Research 2011, 50(4):348-356.
37. Kuhnlein R.P. Thematic review series: Lipid droplet synthesis and metabolism: From yeast to man. Lipid droplet-based storage fat metabolism in Drosophila. Journal of Lipid Research 2012, 53(8):1430-1436.
38. Kulkarni M.M., Booker M., Silver S.J., Friedman A., Hong P., Perrimon N., et al. Evidence of off-target effects associated with long dsRNAs in Drosophila melanogaster cell-based assays. Nature Methods 2006, 3(10):833-838.
39. Ma Y., Creanga A., Lum L., Beachy P.A. Prevalence of off-target effects in Drosophila RNA interference screens. Nature 2006, 443(7109):359-363.
40. Magre J., Delepine M., Khallouf E., Gedde-Dahl T., Van Maldergem L., Sobel E., et al. Identification of the gene altered in Berardinelli-Seip congenital lipodystrophy on chromosome 11q13. Nature Genetics 2001, 28(4):365-370.
41. Min W., Freudiger C.W., Lu S., Xie X.S. Coherent nonlinear optical imaging: Beyond fluorescence microscopy. Annual Review of Physical Chemistry 2011, 62:507-530.
42. Nelliot A., Bond N., Hoshizaki D.K. Fat-body remodeling in Drosophila melanogaster. Genesis 2006, 44(8):396-400.
43. Ni J.Q., Markstein M., Binari R., Pfeiffer B., Liu L.P., Villalta C., et al. Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster. Nature Methods 2008, 5(1):49-51.
44. Payne V.A., Grimsey N., Tuthill A., Virtue S., Gray S.L., Dalla Nora E., et al. The human lipodystrophy gene BSCL2/seipin may be essential for normal adipocyte differentiation. Diabetes 2008, 57(8):2055-2060.
45. Pospisilik J.A., Schramek D., Schnidar H., Cronin S.J., Nehme N.T., Zhang X., et al. Drosophila genome-wide obesity screen reveals hedgehog as a determinant of brown versus white adipose cell fate. Cell 2010, 140(1):148-160.
46. Schneider I. Cell lines derived from late embryonic stages of Drosophila melanogaster. Journal of Embryology and Experimental Morphology 1972, 27(2):353-365.
47. Seip M., Trygstad O. Generalized lipodystrophy. Archives of Disease in Childhood 1963, 38:447-453.
48. Shubeita G.T., Tran S.L., Xu J., Vershinin M., Cermelli S., Cotton S.L., et al. Consequences of motor copy number on the intracellular transport of kinesin-1-driven lipid droplets. Cell 2008, 135(6):1098-1107.
49. Soni K.G., Mardones G.A., Sougrat R., Smirnova E., Jackson C.L., Bonifacino J.S. Coatomer-dependent protein delivery to lipid droplets. Journal of Cell Science 2009, 122(Pt 11):1834-1841.
50. Southall T.D., Elliott D.A., Brand A.H. The GAL4 system: A versatile toolkit for gene expression in Drosophila. Cold Spring Harbor Protocols 2008, pdb to p49.
51. Takashima K., Saitoh A., Hirose S., Nakai W., Kondo Y., Takasu Y., et al. GBF1-Arf-COPI-ArfGAP-mediated Golgi-to-ER transport involved in regulation of lipid homeostasis. Cell Structure and Function 2011, 36(2):223-235.
52. Tian Y., Bi J., Shui G., Liu Z., Xiang Y., Liu Y., et al. Tissue-autonomous function of Drosophila seipin in preventing ectopic lipid droplet formation. PLoS Genetics 2011, 7(4):e1001364.
53. Ulvila J., Parikka M., Kleino A., Sormunen R., Ezekowitz R.A., Kocks C., et al. Double-stranded RNA is internalized by scavenger receptor-mediated endocytosis in Drosophila S2 cells. Journal of Biological Chemistry 2006, 281(20):14370-14375.
54. Villena J.A., Roy S., Sarkadi-Nagy E., Kim K.H., Sul H.S. Desnutrin, an adipocyte gene encoding a novel patatin domain-containing protein, is induced by fasting and glucocorticoids: Ectopic expression of desnutrin increases triglyceride hydrolysis. Journal of Biological Chemistry 2004, 279(45):47066-47075.
55. Welte M.A., Gross S.P., Postner M., Block S.M., Wieschaus E.F. Developmental regulation of vesicle transport in Drosophila embryos: Forces and kinetics. Cell 1998, 92(4):547-557.
56. Yang L., Ding Y., Chen Y., Zhang S., Huo C., Wang Y., et al. The proteomics of lipid droplets: Structure, dynamics, and functions of the organelle conserved from bacteria to humans. Journal of Lipid Research 2012, 53(7):1245-1253.
57. Zimmermann R., Strauss J.G., Haemmerle G., Schoiswohl G., Birner-Gruenberger R., Riederer M., et al. Fat mobilization in adipose tissue is promoted by adipose triglyceride lipase. Science 2004, 306(5700):1383-1386.
58. Zitserman D., Roegiers F. Live-cell imaging of sensory organ precursor cells in intact Drosophila pupae. Journal of Visualized Experiments 2011, 51:e2706.