Purification and characterization of a novel neutral and heat-tolerant phytase from a newly isolated strain Bacillus nealsonii ZJ0702

BMC Biotechnology

Volumn 13, Issue , 2013, Pages

  Yu, Ping ^a   Chen, Yirun ^a  

^a ZHEJIANG GONGSHANG UNIVERSITY   (China)

Author keywords

Bacillus nealsonii; Heat tolerant; Homology analysis; Phytase; Purification and characterization

Indexed keywords

ANTI-NUTRITIONAL FACTORS; HEAT-TOLERANT; HOMOLOGY ANALYSIS; PHYTASES; PROCESSING INDUSTRY; SUBSTRATE SPECIFICITY; THERMALLY STABLE; WIDE TEMPERATURE RANGES;

AMINO ACIDS; BACTERIOLOGY; BIOMOLECULES; CHARACTERIZATION; DNA SEQUENCES; ENZYMES; HYDROLYSIS; THERMAL PROCESSING (FOODS);

PURIFICATION;

BARIUM ION; COBALT; CUPROUS ION; MANGANESE; PHYTASE; ZINC;

AMINO ACID SEQUENCE; ARTICLE; BACILLUS; BACILLUS NEALSONII; BACTERIAL STRAIN; BACTERIUM ISOLATE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME PHOSPHORYLATION; ENZYME SPECIFICITY; MOLECULAR WEIGHT; NONHUMAN; PH; PROTEIN PURIFICATION; THERMOSTABILITY;

BACILLUS NEALSONII;

6-PHYTASE; BACILLUS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; HOT TEMPERATURE; HYDROGEN-ION CONCENTRATION; MOLECULAR WEIGHT; PHOSPHATES; PHYTIC ACID; SEQUENCE ANALYSIS, DNA; SUBSTRATE SPECIFICITY;

EID: 84884603827     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-13-78     Document Type: Article

References (47)

1. Lei XG, Porres JM. Phytase enzymology, applications, and biotechnology. Biotech Lett 2003, 25(21):1787-1794.
2. Wodzinski RJ, Ullah AHJ. Phytase. Advances in Applied Microbiology 1996, 263-302. London Academic Press, Inc, Saul LN, Allen IL.
3. Columbus D, Niven SJ, Zhu CL, de Lange CFM. Phosphorus utilization in starter pigs fed high-moisture corn-based liquid diets steeped with phytase. J Anim Sci 2010, 88(12):3964-3976. 10.2527/jas.2010-3011, 20729280.
4. Cowieson AJ, Ravindran V, Selle PH. Influence of dietary phytic acid and source of microbial phytase on ileal endogenous amino acid flows in broiler chickens. Poultry Sci 2008, 87(11):2287-2299.
5. Dai F, Qiu L, Ye L, Wu D, Zhou M, Zhang G. Identification of a phytase gene in barley (Hordeum vulgare L.). PloS one 2011, 6(4):e18829. 10.1371/journal.pone.0018829, 3080886, 21533044.
6. Farhat A, Chouayekh H, Ben Farhat M, Bouchaala K, Bejar S. Gene cloning and characterization of a thermostable phytase from Bacillus subtilis US417 and assessment of its potential as a feed additive in comparison with a commercial enzyme. Mol Biotech 2008, 40(2):127-135.
7. Maga JA. Phytate: its chemistry, occurrence, food interactions, nutritional significance, and methods of analysis. J Agric Food Chem 1982, 30(1):1-9.
8. Harland BF, Morris ER. Phytate: A good or a bad food component?. Nutr Res 1995, 15(5):733-754.
9. Graf E. Phytic acid: chemistry and applications 1986, USA California: Pilatus Press.
10. Lee DY, Schroeder J, Gordon DT. Enhancement of Cu bioavailability in the rat by phytic acid. J Nutr 1988, 118(6):712-717.
11. Lei X, Stahl C. Biotechnological development of effective phytases for mineral nutrition and environmental protection. Appl Microb Biot 2001, 57(4):474-481.
12. Lei X, Ku PK, Miller ER, Ullrey DE, Yokoyama MT. Supplemental microbial phytase improves bioavailability of dietary zinc to weanling pigs. J Nutr 1993, 123(6):1117-1123.
13. Lei XG, Ku PK, Miller ER, Yokoyama MT, Ullrey DE. Supplementing corn-soybean meal diets with microbial phytase maximizes phytate phosphorus utilization by weanling pigs. J Anim Sci 1993, 71(12):3368-3375.
14. Sebastian S, Touchburn SP, Chavez ER. Implications of phytic acid and supplemental microbial phytase in poultry nutrition: a review. World Poultry Sci J 1998, 54:27-47.
15. Harland BF, Oberleas D. Phytic acid complex in feed ingredients. Phytase in Animal Nutrition and Waste Management: A BASF Reference 1999, Mount Olive: NJ BASF Corp.
16. Yi Z, Kornegay ET, Ravindran V, Denbow DM. Improving phytate phosphorus availability in corn and soybean meal for broilers using microbial phytase and calculation of phosphorus equivalency values for phytase. Poultry Sci 1996, 75(2):240-249.
17. da Silva LG, Trugo LC, da Costa TS, Couri S. Low phytate lupin flour based biomass obtained by fermentation with a mutant of Aspergillus niger. Process Biochem 2005, 40(2):951-954.
18. Vohra A, Satyanarayana T. Phytase production by the yeast, Pichia anomala. Biotech Lett 2001, 23(7):551-554.
19. Vohra A, Rastogi SK, Satyanarayana T. Amelioration in growth and phosphorus assimilation of poultry birds using cell-bound phytase of Pichia Anomala. World J Microb Biotech 2006, 22(6):553-558.
20. Yoo GY, Wang XJ, Choi SY, Han K, Kang JC, Bai SC. Dietary microbial phytase increased the phosphorus digestibility in juvenile Korean rockfish Sebastes schlegeli fed diets containing soybean meal. Aquaculture 2005, 243(1-4):315-322.
21. Fu DW, Huang HQ, Luo HY, Wang YR, Yang PL, Meng K, Bai YG, Wu NF, Yao B. A highly pH-stable phytase from Yersinia kristeensenii: Cloning, expression, and characterization. Enzyme Microb Tech 2008, 42(6):499-505.
22. In MJ, Jang ES, Kim YJ, Oh NS. Purification and properties of an extracellular acid phytase from Pseudomonas fragi Y9451. J Microb Biot 2004, 14:1004-1008.
23. Shah P, Bhavsar K, Soni S, Khire J. Strain improvement and up scaling of phytase production by Aspergillus niger NCIM 563 under submerged fermentation conditions. J Ind Microb Biot 2009, 36(3):373-380.
24. Simon O, Igbasan F. In vitro properties of phytases from various microbial origins. Int J Food Sci Tech 2002, 37(7):813-822.
25. Shimizu M. Purification and characterization of phytase from Bacillus subtilis (natto) N-77. Biosci Biotech Biochem 1992, 56(8):1266-1269.
26. Nampoothiri K, Tomes G, Roopesh K, Szakacs G, Nagy V, Soccol CR, Pankey A. Thermostable phytase production by Thermoascus aurantiacus in submerged fermentation. Appl Biochem Biotech 2004, 118(1):205-214.
27. Pandey A, Szakacs G, Soccol CR, Rodriguez-Leon JA, Soccol VT. Production, purification and properties of microbial phytases. Bioresour Technol 2001, 77(3):203-214. 10.1016/S0960-8524(00)00139-5, 11272007.
28. Bogar B, Szakacs G, Linden JC, Pandey A, Tengerdy RP. Optimization of phytase production by solid substrate fermentation. J Ind Microbiol Biot 2003, 30(3):183-189.
29. Chadha BS, Harmeet G, Mandeep M, Saini HS, Singh N. Phytase production by the thermophilic fungus Rhizomucor pusillus. World J Microb Biot 2004, 20(1):105-109.
30. Singh B, Kaur P, Satyanarayana T. Fungal phytases in ameliorating nutritional status of foods and combating environmental phosphorus pollution. Microbes: Health and Environment 2006, 289-326. New Delhi, India: IK International Publishers, Chauhan AK, Verma A.
31. Lassen SF, Breinholt J, Østergaard PR, Brugger R, Bischoff A, Wyss M, Fuglsang CC. Expression, gene cloning, and characterization of five novel phytases from four Basidiomycete Fungi: Peniophora lycii, Agrocybe pediades, Ceriporia sp., and Trametes pubescens. Appl Environ Microb 2001, 67(10):4701-4707.
32. Maenz DD, Engele-Schaan CM, Newkirk RW, Classen HL. The effect of minerals and mineral chelators on the formation of phytase-resistant and phytase-susceptible forms of phytic acid in solution and in a slurry of canola meal. Anim Feed Sci Tech 1999, 81(3-4):177-192.
33. Oh BC, Choi WC, Park S, Kim YO, Oh TK. Biochemical properties and substrate specificities of alkaline and histidine acid phytases. Appl Microb Biotech 2004, 63(4):362-372.
34. Wyss M, Brugger R, Kronenberger A, Rémy R, Fimbel R, Oesterhelt G, Lehmann M, van Loon APGM. Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolases): catalytic properties. Appl Environ Microb 1999, 65(2):367-373.
35. Cao L, Wang WM, Yang CT, Yang Y, Diana J, Yakupitiyage A, Luo Z, Li DP. Application of microbial phytase in fish feed. Enzyme Microb Tech 2007, 40(4):497-507.
36. Kerovuo J, Lappalainen I, Reinikainen T. The metal dependence of Bacillus subtilis phytase. Biochem Biophy Res Comm 2000, 268(2):365-369.
37. Kim DH, Oh BC, Choi WC, Lee JK, Oh TK. Enzymatic evaluation of Bacillus amyloliquefaciens phytase as a feed additive. Biotech Lett 1999, 21(11):925-927.
38. Boyce A, Walsh G. Purification and characterisation of an acid phosphatase with phytase activity from Mucor hiemalis Wehmer. J Biotech 2007, 132(1):82-87.
39. Fujita J, Yamane YI, Fukuda H, Kizaki Y, Wakabayashi S, Shigeta S, Suzuki O, Ono K. Production and properties of phytase and acid phosphatase from a sake koji mold, Aspergillus oryzae. J Biosci Bioeng 2003, 95(4):348-353.
40. Greiner R, Konietzny U. Phytase for food application. Food Tech Biotech 2006, 44(2):125-140.
41. Quan CS, Tian WJ, Fan SD, Kikuchi JI. Purification and properties of a low- molecular-weight phytase from Cladosporium sp. FP-1. J Biosci Bioeng 2004, 97(4):260-266.
42. In MJ, Seo SW, Kim DC, Oh NS. Purification and biochemical properties of an extracellular acid phytase produced by the Saccharomyces cerevisiae CY strain. Process Biochem 2009, 44(1):122-126.
43. Yu P, Chen YR. Screening and identification of novel phytase-producing Bacillus strain from soil. J Chin Inst Food Sci Tech 2010, 10:116-121.
44. Laemmli UK. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 1970, 227:680-685. 10.1038/227680a0, 5432063.
45. Engelen AJ, Vander HFC, Randsdorp PHG, Smit EL. Simple and rapid determination of phytase activity. J AOAC Int 1994, 77:760-764.
46. Bradford MM. A rapid and sensitive method for the quantization of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976, 72:248-254. 10.1016/0003-2697(76)90527-3, 942051.
47. Fernandez J, DeMott M, Atherton D, Mische SM. Internal protein sequence analysis: enzymatic digestion for less than 10 micrograms of protein bound to polyvinylidene difluoride or nitrocellulose membranes. Anal Biochem 1992, 201(2):255-264. 10.1016/0003-2697(92)90336-6, 1632512.