Purification and properties of an extracellular acid phytase from Pseudomonas fragi Y9451

Journal of Microbiology and Biotechnology

Volumn 14, Issue 5, 2004, Pages 1004-1008

  In, Man Jin ^a   Jang, Eun Seok ^c   Kim, Young Jin ^b   Oh, Nam Soon ^c  

^a Chungwoon University   (South Korea)

^b MCGILL UNIVERSITY   (Canada)

^c Kongju National University   (South Korea)

Author keywords

Extracellular acid phytase; Heterodimer; Pseudomonas fragi

Indexed keywords

2 DIETHYLAMINOETHANOL; COPPER ION; EDETIC ACID; FERROUS ION; PHYTASE; PHYTATE; SEPHAROSE; ZINC ION;

ARTICLE; BACTERIAL STRAIN; BACTERIUM CULTURE; COLUMN CHROMATOGRAPHY; CONTROLLED STUDY; CORRELATION COEFFICIENT; DIMERIZATION; ENZYME ACTIVITY; ENZYME PURIFICATION; ENZYME STABILITY; EXTRACELLULAR SPACE; GEL FILTRATION CHROMATOGRAPHY; MOLECULAR WEIGHT; NONHUMAN; PH MEASUREMENT; POLYACRYLAMIDE GEL ELECTROPHORESIS; PSEUDOMONAS FRAGI; SUPERNATANT;

ANIMALIA; PSEUDOMONAS; PSEUDOMONAS FRAGI;

EID: 8644287569     PISSN: 10177825     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (25)

1. Bradford, M. M. 1976. A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
2. Choi, Y. M., D. O. Noh, S. H. Cho, H. K. Lee, H.-J. Suh, and S.-H. Chung. 1999. Isolation of a phytase-producing Bacillus sp. KHU-10 and its phytase production. J. Microbiol. Biotechnol. 9: 223-226.
3. Gibson, D. M. and A. H. J. Ullah. 1988. Purification and characterization of phytase from cotyledons of germinating soybean seeds. Arch. Biochem. Biophys. 260: 503-513.
4. Greiner, R. 2002. Purification and characterization of three phytases from germinated lupine seeds (Lupinus albus var. Amiga). J. Agric. Food Chem. 50: 6858-6864.
5. Greiner, R., E. Haller, U. Konietzny, and K.-D. Jany. 1997. Purification and characterization of a phytase from Klebsiella terrigena. Arch. Biochem. Biophys. 341: 201-206.
6. Greiner, R., U. Konietzny, and K.-D. Jany. 1993. Purification and characterization of two phytases from Escherichia coli. Arch. Biochem. Biophys. 303: 107-113.
7. Hegeman, C. E. and E. A. Grabau. 2001. A novel phytase with sequence similarity to purple acid phosphatase is expressed in cotyledons of germinating soybean seedling. Plant Physiol. 126: 1598-1608.
8. Heinonen, J. K. and R. J. Lahti. 1981. A new and convenient colorimetric determination of inorganic orthophosphate and its application to the assay of inorganic phosphatase. Anal. Biochem. 113: 313-317.
9. Jeon, J.-H., Y.-J. Han, T.-G. Kang, E.-S. Kim, S.-K. Hong, and B.-C. Jeong. 2002. Purification and characterization of 2,4-dichlorophenol oxidizing peroxidase from Streptomyces sp. AD001. J. Microbiol. Biotechnol. 12: 972-978.
10. Kerovuo, J., M. Lauraeus, P. Nurminen, N. Kalkkinen, and J. Apajalahti. 1998. Isolation, characterization, molecular gene cloning, and sequencing of a novel phytase from Bacillus subtilis. Appl. Environ. Microbiol. 64: 2079-2085.
11. Kim, Y-H., M.-N. Gwon, S.-Y. Yang, T.-K. Park, C.-G. Kim, C.-W. Kim, and M.-D. Song. 2002. Isolation of phytase-producing Pseudomonas sp. and optimization of its phytase production. J. Microbiol. Biotechnol. 12: 279-285.
12. Kim, Y. J., E.-S. Jang, M.-J. In, and N.-S. Oh. 2003. Isolation of phytase producing Pseudomonas fragi and optimization of its phytase production. J. Korean Soc. Agric. Chem. Biotechnol. 46: 291-298.
13. Kim, Y-O., H.-K. Kim, K.-S. Bae, J.-H. Yu, and T.-K. Oh. 1998. Purification and properties of a thermostable phytase from Bacillus sp. DS11. Enzyme Microbial Technol. 22: 2-7.
14. Konietzny, U. and R. Greiner. 2002. Molecular and catalytic properties of phytate-degrading enzymes (phytase). Int. J. Food Sci. Technol. 37: 791-812.
15. Lee, N., J.-M. Lee, K. H. Min, and D. Y. Kwon. 2003. Purification and characterization of 2,3-dihydroxybiphenyl 1,2-dioxygenase from Comamonas sp. SMN4. J. Microbiol. Biotechnol. 13: 487-494.
16. Mullaney, E. J., C. B. Daly, K. Sethumadhavan, E. Rodriquez, X. G. Lei, and A. H. J. Ullah. 2000. Phytase activity in Aspergillus fumigatus isolates. Biochem. Biophys. Res. Commun. 275: 759-763.
17. Shimizu, M. 1992. Purification and characterization of phytase from Bacillus subtilis (natto) N-77. Biosci. Biotechnol. Biochem. 58: 1266-1269.
18. Sohail, S. S. and D. A. Roland. 1999. Influence of supplemental phytase on performance of broilers four to six weeks of age. Poult. Sci. 78: 550-555.
19. Tomschy, A., R. Brugger, M. Lehmann, A. Svendsen, K. Vogel, D. Kostrewa, S. Lassen, D. Burger, A. Kronenberger, A. P. G. M. van Loon, L. Pasamontes, and M. Wyss. 2002. Engineering of phytase for improved activity at low pH. Appl. Environ. Microbiol. 68: 1907-1913.
20. Tye, A. J., F. K. Y, Sin, T. Y. C. Leung, and B. L. Lim. 2002. Molecular cloning and the biochemical characterization of two novel phytases from B. subtilis 168 and B. licheniformis. Appl. Microbiol. Biotechnol. 59: 190-197.
21. Ullah, A. H. J. and D. M. Gibson. 1987. Extracellular phytase (E.C. 3.1.3.8) from Aspergillus ficuum NRRL 3135: Purification and characterization. Prep. Biochem. 17: 63-91.
22. Wyss, M., R. Brugger, A. Kronenberger, R. Rémy, R. Fimbel, G. Oesterhelt, M. Lehmann, and A. P. G. M. van Loon. 1999. Biochemical characterization of fungal phytases (myo-inositol hexakisphosphate phosphohydrolase): Catalytic properties. Appl. Environ. Microbiol. 65: 367-373.
23. Yamada, K., Y. Minoda, and S. Yamamoto. 1968. Phytase from Aspergillus terrus. Part 1. Production, purification, and some general properties of the enzyme. Agric. Biol. Chem. 32: 1275-1282.
24. Yang, W.-J., Y. Matsuda, S. Sano, H. Masutani, and H. Nakagawa. 1991. Purification and characterization of phytase from rat intestinal mucosa. Biochem. Biophys. Acta 1075: 75-82.
25. Zamudio, M., A. Gonzalez, and J. A. Medina. 2001. Lactobacillus plantarum phytase is due to non-specific acid phosphatase. Lett. Appl. Microbiol. 32: 181-184.