Preparation of fosmid libraries and functional metagenomic analysis of microbial community DNA

Methods in Enzymology

Volumn 531, Issue , 2013, Pages 123-142

  Martínez, Asunción ^a   Osburne, Marcia S ^b  

^a Massachusetts Institute of Technology   (United States)

^b TUFTS UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

DNA library; Environmental; Enzymatic assay; Fosmid; Functional screens; High throughput assay; Metagenomic

Indexed keywords

CHITINASE; DNA; NONRIBOSOMAL PEPTIDE SYNTHETASE; PHENOL; PLASMID VECTOR; PROTEINASE K;

ANTIBACTERIAL ACTIVITY; ANTIBIOTIC RESISTANCE; ANTIFUNGAL ACTIVITY; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; BACTERIOPHAGE LAMBDA; CELL GROWTH; CHROMOSOME; COPY NUMBER VARIATION; COSMID; DESICCATION; DNA SEQUENCE; ELECTROPORATION; ENZYME ACTIVITY; EUKARYOTIC CELL; FOSMID; GENE CLUSTER; GENE EXPRESSION; GENE LIBRARY; GENETIC SCREENING; HIGH THROUGHPUT SCREENING; INTRACELLULAR SPACE; METAGENOMICS; MICROBIAL BIOMASS; MICROBIAL COMMUNITY; MICROBIAL GENETICS; MOLECULAR CLONING; MUTATION; NONHUMAN; PIGMENTATION; PRIORITY JOURNAL; RHIZOBIUM LEGUMINOSARUM; SHUTTLE VECTOR;

BACTERIA (MICROORGANISMS);

DNA LIBRARY; ENVIRONMENTAL; ENZYMATIC ASSAY; FOSMID; FUNCTIONAL SCREENS; HIGH-THROUGHPUT ASSAY; METAGENOMIC;

DNA, BACTERIAL; GENETIC VECTORS; GENOMIC LIBRARY; METAGENOMICS; MICROBIAL CONSORTIA;

EID: 84884556433     PISSN: 00766879     EISSN: 15577988     Source Type: Book Series    
DOI: 10.1016/B978-0-12-407863-5.00007-1     Document Type: Chapter

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