Expression and isolation of antimicrobial small molecules from soil DNA libraries

Journal of Molecular Microbiology and Biotechnology

Volumn 3, Issue 2, 2001, Pages 301-308

  MacNeil, I A ^a   Tiong, C L ^a   Minor, C ^a,b   August, P R ^a   Grossman, T H ^a,c   Loiacono, K A ^a,d   Lynch, B A ^a,e   Phillips, T ^a,f   Narula, S ^a   Sundaramoorthi, R ^a   Tyler, A ^a,g   Aldredge, T ^a,h   Long, H ^a   Gilman, M ^a,i   Holt, D ^a,j   Osburne, M S ^a  

^a Aventis Inc   (United States)

^b MILLENNIUM PHARMACEUTICALS INC   (United States)

^c VERTEX PHARMACEUTICALS   (United States)

^d EISAI RESEARCH INSTITUTE   (United States)

^e UCB PHARMA   (Belgium)

^f Massachucetts Biologics Labs   (United States)

^g HARVARD UNIVERSITY   (United States)

^h HARVARD UNIVERSITY   (United States)

ⁱ BIOGEN   (United States)

^j GLAXOSMITHKLINE   (United Kingdom)

more..

Author keywords

[No Author keywords available]

Indexed keywords

ANTIINFECTIVE AGENT; BACTERIAL DNA; DNA FRAGMENT; INDIRUBIN; NATURAL PRODUCT;

ANTIMICROBIAL ACTIVITY; ARTICLE; BIODIVERSITY; CLONING; DNA LIBRARY; DRUG SCREENING; GENE EXPRESSION; GENE ISOLATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MOLECULAR BIOLOGY; NONHUMAN; PARTICLE SIZE; PHYLOGENY; SOIL MICROFLORA; THIN LAYER CHROMATOGRAPHY;

AMINO ACID SEQUENCE; ANTI-BACTERIAL AGENTS; CHROMOSOMES, ARTIFICIAL, BACTERIAL; DNA, BACTERIAL; DNA, RIBOSOMAL; ENZYMES; ESCHERICHIA COLI; GENE LIBRARY; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; OPEN READING FRAMES; PHYLOGENY; POLYMERASE CHAIN REACTION; RNA, RIBOSOMAL, 16S; SOIL MICROBIOLOGY;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI;

EID: 0035079979     PISSN: 14641801     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (46)

1. Sequence analysis and functional characterization of the violacein biosynthetic pathway from Chromobacterium violaceum
2. Molecular phylogeny of Archaea from soil
3. Diversity and association of psychrophilic bacteria in Antarctic sea ice
4. Construction and characterization of a bovine bacterial artificial chromosome library
5. Natural products as a resource for new drugs
6. Search and discovery strategies for biotechnology: The paradigm shift
7. Antineoplastic agents from natural sources: Achievements and future directions
8. A Streptomyces avermitilis gene encoding a 4-hydroxyphenylpyruvic acid dioxygenase-like protein that directs the production of homogentisic acid and an ochronotic pigment in Escherichia coli
9. Modular organization of genes required for complex polyketide biosynthesis
10. Formation of indigo and related compounds from indolecarboxylic acids by aromatic acid-degrading bacteria: Chromogenic reactions for cloning genes encoding dioxygenases that act on aromatic acids
11. Broad-scale approaches to the determination of soil microbial community structure: Application of the community DNA hybridization technique
12. Highlight on the studies of anticancer drugs derived from plants in China
13. Molecular biological access to the chemistry of unknown soil microbes: A new frontier for natural products
14. Identification of indigo-related pigments produced by Escherichia coli containing a cloned Rhodococcus gene
15. Strategies for discovering drugs from previously unexplored natural products
16. Indirubin, the active constituent of a Chinese antileukaemia medicine, inhibits cyclin-dependent kinases
17. Impact of culture-independent studies on the emerging phylogenetic view of bacterial diversity
18. Organization of the biosynthesis genes for the peptide antibiotic gramicidin S
19. Retrofitting vectors for Escherichia coli-based artificial chromosomes (PACs and BACs) with markers for transfection studies
20. A study of the contrasting effects of cyclosporine, FK 506, and rapamycin on the suppression of allograft rejection
21. Indigo formation by microorganisms expressing styrene monooxygenase activity
22. Tapping into microbial diversity for natural products drug discovery
23. An improved approach for construction of bacterial artificial chromosome libraries
24. A molecular view of microbial diversity and the biosphere
25. An effective method to extract DNA from environmental samples for polymerase chain reaction amplification and DNA fingerprint analysis
26. Cloning the soil metagenome: A strategy for accessing the genetic and functional diversity of uncultured microorganisms
27. The Earth's bounty: Assessing and accessing soil microbial diversity
28. Purification, nucleotide sequence and some properties of a bifunctional isomerase/decarboxylase from the homoprotocatechuate degradative pathway of E. coli
29. The biosynthetic gene cluster for the polyketide immunosuppressant rapamycin
30. Cloning and stable maintenance of 300-kilobase-pair fragments of human DNA in Escherichia coli using an F-factor-based vector
31. Screening of natural products for antimicrobial agents
32. Artificial chromosomes for antibiotic-producing actinomycetes
33. The natural history of microorganisms
34. Characterization of a Yellowstone hot spring microbial community by 5S rRNA sequences
35. Construction of a 750-kb bacterial clone contig and restriction map in the region of human chromosome 21 containing the progressive myoclonus epilepsy gene
36. The role of natural products in a modern drug discovery program
37. Bacterial diversity among small-subunit rRNA gene clones and cellular isolates from the same seawater sample
38. Interference of humic acids and DNA extracted directly from soil in detection and transformation of recombinant DNA from bacteria and a yeast
39. Comparison of phenotypic diversity and DNA heterogeneity in a population of soil bacteria
40. Rapid method for separation of bacterial DNA from humic substances in sediments for polymerase chain reaction
41. 16S rRNA sequences reveal numerous uncultured microorganisms in a natural community
42. 16S ribosomal DNA amplification for phylogenetic study
43. Prokaryotes: The unseen majority
44. Cloning and characterization of a Pseudomonas mendocina KR1 gene cluster encoding toluene-4-monooxygenase
45. DNA recovery from soils of diverse composition