Substrate-induced gene-expression screening of environmental metagenome libraries for isolation of catabolic genes

Nature Biotechnology

Volumn 23, Issue 1, 2005, Pages 88-93

  Uchiyama, Taku ^a   Abe, Takashi ^b,c   Ikemura, Toshimichi ^b   Watanabe, Kazuya ^a  

^a MARINE BIOTECHNOLOGY INSTITUTE   (Japan)

^b GRADUATE UNIVERSITY FOR ADVANCED STUDIES   (Japan)

^c Xanagen Inc   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

CLONING; ENZYMES; SCREENING;

CATABOLIC GENES; ISOLATION;

GENES;

ARTICLE; BIOTECHNOLOGY; DNA SEQUENCE; ENZYME ACTIVITY; ESCHERICHIA COLI; FLUORESCENCE ACTIVATED CELL SORTING; GENE EXPRESSION; GENE LIBRARY; GENETIC PROCEDURES; GENETIC SCREENING; HIGH THROUGHPUT SCREENING; MICROBIAL GENETICS; MOLECULAR CLONING; NONHUMAN; NUCLEOTIDE SEQUENCE; OPEN READING FRAME; OPERON; PRIORITY JOURNAL; SPECTROPHOTOMETRY; UNINDEXED SEQUENCE; CATALYSIS; CELL SEPARATION; CHEMISTRY; ENVIRONMENT; FLOW CYTOMETRY; GENE VECTOR; GENETIC DATABASE; GENOME; METABOLISM; METHODOLOGY; MOLECULAR GENETICS; PHYLOGENY; PLASMID; RESTRICTION FRAGMENT LENGTH POLYMORPHISM;

ESCHERICHIA COLI;

COMPLEMENTARY DNA; GREEN FLUORESCENT PROTEIN; PEPTIDE LIBRARY;

BIOTECHNOLOGY; CATALYSIS; CELL SEPARATION; CLONING, MOLECULAR; DATABASES, GENETIC; DNA, COMPLEMENTARY; ENVIRONMENT; ESCHERICHIA COLI; FLOW CYTOMETRY; GENE EXPRESSION; GENE LIBRARY; GENETIC TECHNIQUES; GENETIC VECTORS; GENOME; GREEN FLUORESCENT PROTEINS; MOLECULAR SEQUENCE DATA; OPEN READING FRAMES; OPERON; PEPTIDE LIBRARY; PHYLOGENY; PLASMIDS; POLYMORPHISM, RESTRICTION FRAGMENT LENGTH; SEQUENCE ANALYSIS, DNA; SPECTROPHOTOMETRY;

EID: 19144369096     PISSN: 10870156     EISSN: 15461696     Source Type: Journal    
DOI: 10.1038/nbt1048     Document Type: Article

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