A VL single-domain antibody library shows a high-propensity to yield non-aggregating binders

Protein Engineering, Design and Selection

Volumn 25, Issue 6, 2012, Pages 313-318

  Hussack, Greg ^a,b   Keklikian, Artine ^b   Alsughayyir, Jawaher ^a,b   Hanifi Moghaddam, Pejman ^a,c   Arbabi Ghahroudi, Mehdi ^a,d   Van Faassen, Henk ^a   Hou, Sheng T ^a,b   Sad, Subash ^a,b   MacKenzie, Roger ^a,c   Tanha, Jamshid ^a,b,c  

^a NATIONAL RESEARCH COUNCIL CANADA   (Canada)

^b UNIVERSITY OF OTTAWA   (Canada)

^c UNIVERSITY OF GUELPH   (Canada)

^d CARLETON UNIVERSITY   (Canada)

Author keywords

human single domain antibody; non aggregating; synthetic phage display library; VH; VL

Indexed keywords

ANTIBODY LIBRARY; COMPLEMENTARITY-DETERMINING REGIONS; NON-AGGREGATING; PHAGE DISPLAY LIBRARIES; SINGLE DOMAINS;

ANTIBODIES; ANTIGENS; SCAFFOLDS;

BINDERS;

ANTIBODY; BACTERIAL ANTIGEN; BACTERIAL PROTEIN; BACTERIAL TOXIN; IMMUNOGLOBULIN LIGHT CHAIN; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY; TOXB PROTEIN, CLOSTRIDIUM DIFFICILE;

ANTIBODY LIBRARY; ANTIGEN EXPRESSION; ARTICLE; CODON; CONTROLLED STUDY; DISSOCIATION; ENZYME LINKED IMMUNOSORBENT ASSAY; HUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; SCANNING ELECTRON MICROSCOPY; SOLUBILITY; AMINO ACID SEQUENCE; ANTIBODY AFFINITY; CHEMISTRY; GENETICS; METABOLISM; MOLECULAR CLONING; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PEPTIDE LIBRARY; PROTEIN BINDING;

AMINO ACID SEQUENCE; ANTIBODY AFFINITY; ANTIGENS, BACTERIAL; BACTERIAL PROTEINS; BACTERIAL TOXINS; BASE SEQUENCE; CLONING, MOLECULAR; ENZYME-LINKED IMMUNOSORBENT ASSAY; HUMANS; IMMUNOGLOBULIN LIGHT CHAINS; MOLECULAR SEQUENCE DATA; PEPTIDE LIBRARY; PROTEIN BINDING; SINGLE-CHAIN ANTIBODIES;

EID: 84861603491     PISSN: 17410126     EISSN: 17410134     Source Type: Journal    
DOI: 10.1093/protein/gzs014     Document Type: Article

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