Isolation of antigen-binding camelid heavy chain antibody fragments (nanobodies) from an immune library displayed on the surface of Pichia pastoris

Journal of Biotechnology

Volumn 145, Issue 2, 2010, Pages 93-98

  Ryckaert, Stefan ^a,b   Pardon, Els ^a,c   Steyaert, Jan ^a,c   Callewaert, Nico ^a,b  

^a DEPARTMENT OF PLANT SYSTEMS BIOLOGY   (Belgium)

^b GHENT UNIVERSITY   (Belgium)

^c VRIJE UNIVERSITEIT BRUSSEL   (Belgium)

Author keywords

Camelid heavy chain antibody; Nanobody; Pichia pastoris; Surface display

Indexed keywords

ANTIBODY FRAGMENT; ANTIGEN-BINDING; CODING SEQUENCES; EQUILIBRIUM DISSOCIATION CONSTANT; EUKARYOTIC EXPRESSION; FLUORESCENCE ACTIVATED CELL SORTING; GREEN FLUORESCENT PROTEIN; HEAVY CHAIN; HEAVY-CHAIN ANTIBODIES; HEAVY-CHAIN ANTIBODY FRAGMENTS; HIGH EFFICIENCY TRANSFORMATION; NANOBODIES; P. PASTORIS; PICHIA PASTORIS; PROTEIN LIBRARY; SACCHAROMYCES CEREVISIAE; SURFACE DISPLAYS;

DISSOCIATION; EQUILIBRIUM CONSTANTS; FLUORESCENCE; YEAST;

ANTIBODIES;

ALPHA AGGLUTININ; ANTIBODY; CAMELID HEAVY CHAIN ANTIBODY FRAGMENT; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; NANOBODY; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ANTIBODY ENGINEERING; ANTIBODY ISOLATION; ANTIBODY LIBRARY; ANTIBODY PRODUCTION; ANTIGEN BINDING; ARTICLE; ARTIODACTYLA; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DISSOCIATION CONSTANT; FLUORESCENCE ACTIVATED CELL SORTING; FUSION GENE; GENE EXPRESSION; MOLECULAR CLONING; NONHUMAN; PHAGE DISPLAY; PICHIA PASTORIS; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; YEAST SURFACE DISPLAY;

ANIMALS; ANTIBODIES, MONOCLONAL; CAMELIDS, NEW WORLD; CELL MEMBRANE; CLONING, MOLECULAR; IMMUNOGLOBULIN HEAVY CHAINS; IMMUNOGLOBULIN VARIABLE REGION; PEPTIDE LIBRARY; PICHIA; PROTEIN ENGINEERING;

CAMELIDAE; EUKARYOTA; LAMA (MAMMAL); PICHIA PASTORIS; SACCHAROMYCES CEREVISIAE;

EID: 72949123016     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2009.10.010     Document Type: Article

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